Abstract:Allozyme variation was examined in 48 pomelo cultivars from the collection nursery of the Chinese Academy of Agricultural Sciences. Ten loci observed in 5 enzyme systems, Esterase (EST), Phosphoglucoisomerase (PGI), Phosphoglucomutase (PGM), Shikimate dehydrogenase (SKD), and Superoxide dismutase (SOD) were encoded by 25 alleles. Except for 2 loci of PGI, all other 8 loci were polymorphic. Effective number of alleles per locus was l.55, and the expected heterozygosity was 0.2805, Which were comparatively low． Pomelo culfivars are rich in aUozymic genetic diversity．Lower level of total germplasm diversity is probably due to a long history of artificial sdeetion and gene loss．