TISSUE CULTURE AND ESTABLISHMENT OF REGENERATION SYSTEM OF DELPHINIUM GRANDIFLORUM

Abstract:

The explants from seed and leaf segments of Delphinium grandiflorum were used to establish culture in vitro and regeneration system. The experiments showed that most explants induced calli, few of them could form plantlets directly from seed or leaf segments. Under suitable culture condition, high regeneration rates could be obtained from explants of seed and leaf segments, each explant regenerating 10-20 plantlets. Optimum medium for seed segments of Delphinium grandiflorum was the modified MS medium (half of macronutrients and sucrose，10 times the amount of Vit B₁) supplemented with 3 mg L^-1 ZT and 0.3 mg L^-1 NAA，and for leaf segments，the medium of MS with 3 mg L^-1 6-BA and O.3 mg L^-1 NAA．