Abstract:Explants from dormant bud segments and from epicotyls and hypocotyl of the seedlings of Michelia maudiae Dunn were cultured on MS medium supplemented with various concentrations of NAA, 2,4-D, BA alone or in combination. Medium with 2,4-D could only produce calluses. A large number of adventitious buds and some calluses were observed on medium containing BA (3.0 mg L-1) and NAA (0.2 mg L-1). Calluses from original explants generated a maximum number of shoots in subculture on MS medium supplemented with BA (2.0 mg L-1)and NAA (0.2 tag L-1)．Planflets rooted well on 1/2 Ms medium containing 0.5 mg L NAA with 1.0％ active carbon． The plantlets were transferred to pots containing a perlite/coconut fibre and (2：2：1) mixture which grew normally with a survival rate of 92％．