A short photoperiod and low temperature-induced male sterile rice Yi-DIS was crossed With some rice varieties to examine the pollen fertility of parents and F1 under natural low temperature, and to investigate the fertility segregation of F2 and F3 during stable phase in sterile line. The results showed that the stability of fertility in parents and F1 was affected to a certain extent by low temperature. Besides the sensitivity of male parent to low temperature, pollen fertility in F1 was also related to some other factors，and the fertility stability in F1 might result from heredity，The characteristics of male sterile Yi-DIS basically accorded with genetic mode controlled by two pairs of independent major genes，and was affected probably by many minor genes． It seems that the two pairs of restoring genes corresponding to this two pairs of major genes were different in genetic effect．