Abstract:In this paper, we used modified CTAB method to extract genomic DNA from litchi for amplifying RAPD markers. We got 37 polymorphic RAPDs out of 259 total RAPDs from 34 litchi cultivars with 50 random primers. The results showed that these 34 cultivars could be divided into 4 groups. Group I included "Zhuangyuanhong", "Yuanhong", "Guilin", "Songjiaxiang", "Taiwanli", "Xiafanzhi", "Dongliu No. l", "Feizixiao", "Baitangying", "Xiangwan", "Lusha". Group II comprised "Wulizi", "Shuidong", "Wuye", "Caijiawanzi","Qingkenuomici"," Nuomici",Group III included "Guiwei", "Guangming"," Bailizhi","Chenzi" , "Lanzhu", "Jidi", "Taslniu". Group Ⅳ consisted of "Huitoujiang","Putaoben", "HuaizhV", "Nanhaili", "Dazao", "Zaohong"，"Huoshaoli", "Wuyejiu"，"Midingxlang", "Luhebao". The results of cluster suggested that the polymorphism of litchi germplasm was narrower than expected.