Abstract:The contents of endogenous IAA, ABA, iPAs, ZRs, GA1+3, GA4+7 and ethylene in three phenotypes of cucumber hairy roots transformed by Ri T-DNA from Agrobacterium rhizogenes R1000 were assayed by enzyme-linked immunosorbent assay (ELISA) and gas chromatography, respectively. The 3 phenotypes of cucumber hairy roots were described by the authors and published in Acta Botanica Sinica 1998, 40(5).470-473. The results showed that the contents of endogenous IAA, ABA and GAl +3 in hairy roots were highest in phenotype III，and less in descending order in phenotype II and phenotype I，whereas the amount of GA4+ 7 was in oppsite order，GA4+ 7 content in hairy roots of phenotype III was lowest and could not be detected．The contents of iPAs and GAl+3 in control roots and RlO00-transformed hairy roots were much higher than those of ZRs and GA4+ 7，respectively．However，the contents of iPAs and ZRs in R1000-transformed hairy roots were higher than those in control roots．The ability of ethylene production in R1000-transformed hairy roots was much higher than that in cucumber control roots．