SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM AXES OF PEANUT EMBRYOS
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    Abstract:

    Somatic embryos could be induced from axes of mature peanut embryos culturedon hasal MS medium containing high concentration of 2,4-D (40 mg L-1) with 0.5 mg L-1 kinetin, 30% sucrose and 0.9% agar for 30 days, the induction rate being 100 percent,and the mean embryo number per explant reaching 11.58. Lower concentrations of 2,4-D(10-20 mg L-1) were suitable for somatic embroid differentiation in subculture. However,10 mg L-1 2,4-D was the optimum concentration for inducing somatic embryos both in primary culture and in subculture by immature embryo axes.Somatic embryos could easily germinate on MS medium with 5-10 mg L-1 BA. The plantiets were obtained after the shoots were tranferred to root-inducing medium, MS+ 2 mg L-1 NAA.

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庄伟建, 张书标, 刘思衡, 蔡来龙.花生体细胞胚的诱导及其植株再生[J].热带亚热带植物学报,1999,7(2):153~158

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