Abstract:Four-leaf seedlings of tobacco were used to study the resistance to anthracnose disease caused by Colletotrichum desructivum O' Gara infection. After or before treatment in seedlings sprayed with 1 mg ml-1 methyl jasmonate (MJ) one time a day for 3 days, fungous spores (108 ml-1) were inoculated to the leaves by spraying 2 times at an interval of 12 hours. The numbers of lesion of leaves were recorded and pathogenesis-related proteins (PRP) were determined after treatment for 7 to 15 days. When tobacco seedlings were inoculated with spores, the resistibility was quite different among three varieties. Brazil variety showed stronger resistance to this disease than K326 and Guanghuang55. Treating with MJ before spore inoculation, the infection symptom in seedlings was reduced, indicating that MJ can induce the resistance of tobacco to fungous infection. The PRP contents of seedlings increased after treating with MJ or inoculating with spores. The correlation coefficient between PRP of Brazil variety and infection degree was -0.9958. It is suggested that PRP is significantly related to fungus resistance in tobacco seedlings of this variety.