CHANGES IN ACTIVITIES OF PROTEOLYTIC ENZYMES IN HUBEI PHOTOPERIOD SENSITIVE GENIC MALESTERILE RICE AT THE STAGES OF PANICLE AND POLLEN DEVELOPMENT
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    Abstract:

    The content of soluble protein in panicle of Hubei photoperiod sensitive genic male-sterile rice, Nongken 58S, was neariy the same in both fertile and sterile plants at the stage of first panicle branch differentiation. Soluble protein contents in fertile plant at the stage of pistil and stamen formation and the stage of pollen mother cell differentiation were higher than those in the sterile one by 21% and 12.3%, respectively. Higher soluble protein content occurred at binucleate and trinucleate stages for the fertile plant. The activity of azocasein protease increased rapidly at the stage of pistil and stamen formation in male-sterile plant, whereas this process was not present in fertile plant until binucleate stage. Male-sterile plant had lower endopeptidase activity at the stage of second panicle branch differentiation, and higher activity of this enzyme appeared at other stages as compared with the fertile plant. The endopeptidase patterns changed with the fertility. Higher aminopeptidase was observed in the fertile plant of rice.

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孙谷畴,林植芳,林桂珠,梁承邺.湖北光周期敏感核不育水稻农垦58S幼穗和花药发育期的蛋白酶活性变化[J].热带亚热带植物学报,1997,5(2):61~67

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