Abstract:Buds of Acacia auriculiformis and A.mangium from 4-year-old mother trees were first sterilized in 6% chloros for 20 minutes,and then in 0.1% mercuric chloride for 5 minutes.Multiple shoot induction was obtained through culturing on MS basal medium supplemented with 10μmol/L BA and 0.5μmol/L IBA.Large explants were easier to induce multiple shoots than small ones.66.7% of large explants of A. auriculiformis and 60.0% of A.mangium explants produced multiple shoots,the largest shoot number per explant reached 55 and 48 respectively.Root induction was done on half concentration of MS inorganic compounds supplemented with formal MS organic compounds, 4.92-7.38μmol/L IBA and 2.69μmol/L NAA medium.Rooting percentages of A.auriculiformis and A. mangium shoots were 29.8% and 25.0%,respectively.The frequency of rooting also related with shoot's health and its physiological state.Subculture can rejunvenate plant tissue,increase the number of shoot per explant and promote rooting.Vigorous plantlet can be obtained when the rooted-shoots were transplanted to soil after two-week training.