SOME REGULATIONS OF SOMATIC EMBRYOGENESIS AND ORGANOGENESIS IN INDICA RICE
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    Abstract:

    Different concentrations (0, 0. 2, 0. 5, 1. 0 and 2. 0 mg/L) of 2, 4-D and kinetin (25combinations of them) in the media were studied for inducing embryoSenesis and orpoogenesisfrom young Panicle of rice. When the medium contained both 2, 4-D and kinetin, the plantletswere regenerated via somatic embryogenesis. When the medium contained 2, 4-D only or kinetinonly, plantlets were regenerated via organogenesis but the former was adventitious bud fromorganogenic callus and the later, from the explant directly. In subCulture condition, the mediumcontaining both 2, 4-band kinetin could maintain the culture keeping a high flequency for embryogenesis and the medium with 2, 4--D only, for organogenesis. Frequency of plant regeneration, duration of keeping the capacity for plant regeneration and number of regenerated plant Percallus in embryogenesis wele hiSher, longer and more than those in organogenesis respectively.Embryogenic callus consisted of embryoids, which was made up coleoptile, coleorhiza and scutellum, POSSessed the structure of distinct and POlylayers. The orgnnogenic callus did not aness thisorganletS and friable in structure.

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凌定厚,驹岭穆.籼稻体细胞胚胎发生及器官发生的比较研究[J].热带亚热带植物学报,1994,2(1):70~78

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