ELECTRQPHORETIC DRIVE: HIGH EFFICIENCY GENE TRANSFER USING A NOVEL APPROACH FOR THE DELIVERY OF FOREIGN GENES INTO RICE CELLS
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    Abstract:

    A new method has been developed to introduced foreign DNA into rice cells. Gene delivery occurs when a low-voltage electrophoresis at long pulse applied to a mixture containing partially digested SCG (small cell groups) and plasmid DNA. Gene-transfer efficiency is evaluated by a detection of GUS activity resulting from the chimeric plasmid DNA. The optimal combination of voltage and pulse produces a frequency of up to 8. 2% for transformation of SCG. The results demonstrates that electrophoretic drive is applicable to introduce foreign genes into plant cells.

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杨金水,葛扣麟,王蕴珠,王蓓,谈家桢.低压脉冲电泳介导的外源基因转化部分酶解的水稻小细胞团[J].热带亚热带植物学报,1992,0(1):115~122

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