Abstract:Calli from leaf explants of two cultivars of Pogostemon cablin (Blance) Benth., which derived from Shipai in Guangzhou and Hainan, respectively, were obtained on Murashige Skoog (MS) medium supplemented with 2,4-D (0.05 mg L-1) and KT(0.5 mg L-1), and plantlets regenerated through callus were obtained on MS medium supplemented with 6-BA (1 mg L-1). Essential oils were extracted from the regenerated plant and the constituents were analyzed using GC method. The results showed that there were obviously morphological differences in regenerated plants of the two cultivars after cultured for 2~3 months. Essential oil content in cultivar from Shipai was 1.4％，lower than that from Hainan, which was 2.9%, but the content of pogostone in cultivar of Shipai, being 375.76 mg ml-1, was significantly higher than that from Hainan, which was 7.82 mg ml-1. These differences exsited in the initial plants. This results provided more scientific basis for the classification of Pogostemon cablin.