Volume 32,2024 Issue 5

Plant Ecology and Natural resource Management

• Population Structure and Dynamic Analysis of Wild Cathaya argyrophylla in Dashahe National Nature Reserve, Guizhou, China

  WANG Linjin, YANG Rui, HAN Jihuai, GOU Wei, ZHAO Yangyang, GAO Xu

  2024,32(5):571-578, DOI: 10.11926/jtsb.4825

  Abstract: (82) HTML (41) PDF (614.90 KKB)(2167)

  Abstract:

  Cathaya argyrophylla is one of the endemic rare and endangered wild plant with extremely small population in China, and is also the key protected object and flagship species in Guizhou Dashahe National Nature Reserve. Based on survey data of wild C. argyrophylla in the reserve in 2022, the quantitative dynamic index was calculated, static life table was compiled, survival curve was drawn, and survival function and spectral analysis were introduced to describe its structural dynamics. The results showed that the age structure of wild C. argyrophylla population was inverted J-type, and the dynamic change index of population (V_pi) was 39.43%, which was greater than V_pi' and both of them were greater than 0, indicating that the C. argyrophylla population was a growth type, but the growth was slow, and it was sensitive to external disturbance. The survival curve of the population belonged to Deevey-II type, and the survival rate, cumulative mortality rate, mortality density and risk rate changed greatly in age class from I to III stage, and then tended to be stable. The life spectral analysis showed that the III age class was the main stage affecting C. argyrophylla population. Therefore, the large number of young individuals and high mortality rates of C. argyrophylla in the reserve was the main reason leading to the difficulties of regeneration. It was suggested that the reserve should strengthen the care of C. argyrophylla seedlings and improve the living environment, so as to promote the natural regeneration and recovery of C. argyrophylla population.

• Population Distribution Pattern and Quantitative Dynamic Characteristics of Endangered Wild Paphiopedilum appletonianum

  XIA Dan, WANG Weifeng, LI Donghai, LI Yu, ZHANG Shunwei, YANG Xiaobo

  2024,32(5):579-588, DOI: 10.11926/jtsb.4817

  Abstract: (44) HTML (35) PDF (698.89 KKB)(2131)

  Abstract:

  Paphiopedilum appletonianum is an endangered and class I protected orchid. In order to understand the status of its wild population and promote the protection of wild population, two large populations distributed in Nangaoling and Bawangling in Hainan were investigated in the field. The population distribution pattern was analyzed by point pattern method, and the population dynamic characteristics was analyzed through static life table, survival curve analysis, survival analysis, population reproduction strategy analysis and fertility table. The results showed that the distribution pattern of the two populations changed from aggregation to random and uniform distribution as the scale increased. The Bawangling population was mainly clustered at different scales, while the Nangaoling population was mainly random and even distributed. The age structure of the two populations was pyramid shape, and the survival curve was Deevey-II type, showing a decline trend with the increase of age class. There were two reproductive strategies: sexual reproduction and clone reproduction, and the asexual reproduction of Bawangling population was stronger, and the sexual reproduction of Nangaoling population was stronger. Therefore, Bawangling population was a growing population, while Nangaoling population was a slow negative increasing population.

• Characteristics of Bryophyte Community in Wetland Park and Its Relationship with Environment Factors: A Case Study of Guiyang City

  DUAN Lixin, WANG Xiurong, LIAO Fang, XIE Muyan

  2024,32(5):589-600, DOI: 10.11926/jtsb.4800

  Abstract: (50) HTML (37) PDF (2.43 MKB)(2108)

  Abstract:

  As one of the plant components of wetland park, the study of bryophyte community construction can provide important theoretical basis for wetland ecosystem management and biodiversity maintenance mechanism. Guiyang is rich in wetland and bryophyte resources. Based on three typical wetland parks in Guiyang, the species distribution of bryophytes and its relationship with environmental factors were studied. TWINSPAN analysis and canonical correspondence analysis (CCA) were used to quantitatively classify and rank the bryophytes community. The results showed that there were 17 families, 28 genera and 66 species of bryophytes in three typical wetland parks, among which Pottiaceae, Hypnaceae and Brachytheciaceae were dominant families. There was little difference in β diversity of bryophytes among the three wetland parks, with high species similarity and low richness. The habitats of bryophytes were mainly lithophyte and arboreal. The life forms were mainly interleaved and clustered. Bryophyte community could be divided into 13 types, all of which had their own characteristics, and their species composition and community characteristics could better reflect the relationship between the bryophyte type and environment factors. The habitats of the three wetland parks were similar. The results of CCA analysis showed that the primary environmental factor affecting the distribution of moss clusters in wetland park was altitude, followed by air humidity, canopy density, and light also had a certain effect. These provide a scientific basis for the conservation of bryophyte diversity in wetland park.

• Interspecific Associations and Community Stability Changes of Dominant Woody Species of Natural Forest Communities in Mulinzi National Nature Reserve

  CHEN Jinshi, GUO Qiuju, YAO Lan, ZHU Jiang, LIU Xiyao, XIANG Qin, ZHAO Huandun, AI Xunru

  2024,32(5):601-610, DOI: 10.11926/jtsb.4815

  Abstract: (53) HTML (31) PDF (995.33 KKB)(2126)

  Abstract:

  In order to explore the changes of natural forest community structure in the national nature reserve, fourty-five 20 m×20 m plots were set up in three restoration stages, including original old forest (restored for 100 years or more), secondary forest restored for 40 and 25 years after clear-cutting. The interspecific association and community stability of dominant woody plants were studied by using Variance ratio (VR), χ² test, Pearson correlation test, Spearman rank correlation test and M-Godron stability test. The results showed that there was no significant positive association on the whole of dominant woody species in secondary forest restored for 25 and 40 years, but the significant positive association appeared in original aged forest restored for 100 years. The χ² test showed that the interspecific associations were not significant in 25 and 40 years plant communities, and only 2 species pairs in 100 years plant communities were significantly associated, and the interspecific associations were loose. The Pearson’s correlation coefficient test and Spearman’s rank correlation coefficient test also showed that the correlations among species pairs of most species were not significant, and displayed an independent distribution pattern. The M-Godron stability analysis showed that the plant community was in an unstable state at different stages, but the stability of the community gradually increased with restoration time. Therefore, interspecific correlation in the natural forest communities was weak at different restoration stages, and the species distributed independently and changed frequently, indicating that the community was still in the process of succession and was developing towards a stable stage.

• Effect of Warming on Dissolved Organic Matter in Litter of Subtropical Evergreen Broad-leaved Forest

  WANG Yun, ZHANG Yuhui, RAN Jiaxin, MAO Chao, XIONG Decheng, CHEN Shidong, YANG Zhijie

  2024,32(5):611-619, DOI: 10.11926/jtsb.4831

  Abstract: (43) HTML (39) PDF (679.20 KKB)(2110)

  Abstract:

  In order to understand the effect of warming on litter decomposition, the litter collected in subtropical evergreen broad-leaved forest was distributed in control and warming (+4 ℃) plots, respectively. The changes in the quantity and spectral characteristics of dissolved organic matter during litter decomposition under warming were studied. The results showed that the mass residual rate of litter decreased significantly with decomposition process, rapidly decreased by 20.08% to 23.32% in 0-60 days, and slowly decreased by 6.35% to 10.98% in 60-210 days. Along decomposition process, the dissolved organic carbon content of litter source showed a decreasing trend of first fast and then slow, and the overall decrease was 94.15%. The content of dissolved organic nitrogen fluctuating decreased by 81.82% overall. The spectral characteristics of dissolved organic matter (SUVA₂₅₄, SUVA₂₆₀, SUVA₂₈₀ and SUVA₃₇₀) showed an increase-decrease-increase tendency. The warming did not significantly affect the mass residual rate of litter and the spectral characteristics of dissolved organic matter, but the contents of dissolved organic carbon and organic nitrogen decreased by 16.72% and 25.10%. Therefore, the decomposition of litters decreased the mass residual rate of litters and the contents of dissolved organic carbon and organic nitrogen, and changed the spectral characteristics of dissolved organic matter. Although warming decreased the contents of dissolved organic carbon and organic nitrogen in litters, it did not significantly change the mass residual rate and spectral characteristics of dissolved organic matter in litters.

• Plasticity Response of Fine Root Morphological Traits to Short-term Nitrogen and Phosphorus Addition in Subtropical Cunninghamia lanceolata and Castanopsis carleisii Plantations

  ZHENG Ziyi, JIANG Qi, JIA Linqiao, CHEN Guangshui

  2024,32(5):620-628, DOI: 10.11926/jtsb.4803

  Abstract: (34) HTML (30) PDF (674.97 KKB)(2123)

  Abstract:

  Taking similar age arbuscular mycorrhiza (AM) species Cunninghamia lanceolata and ectotrophic mycorrhiza (EM) species Castanopsis carlesii in Sanming, Fujian Province as the research objects, the effects of nitrogen and phosphorus addition on morphological characteristics of fine roots of different mycorrhizal species were studied. The results showed that the diameter and root tissue density of fine roots of Cunninghamia lanceolata had significantly positive N and P plasticity response, and the specific root length and specific surface area had negative plasticity response, while the morphological traits of fine roots of Castanopsis carlesii showed opposite plasticity response. The P plasticity response of fine root morphological traits of Cunninghamia lanceolata was stronger than that of Castanopsis carlesii, but there was no significant difference between N and P plastic responses of fine root morphological traits of Castanopsis carlesii. The direction of plasticity response of the same morphological trait of fine root to N and P was the same, and there was a positive correlation. There was synergistic and tradeoff relationship between nutrient plasticity responses of different morphological characters in fine roots. Therefore, AM species Cunninghamia lanceolata adopted the resource conservation strategy to prolong the fine rootlife, while the EM species Castanopsis carlesii adopted the resource acquisition strategy to occupy resources quickly. In addition, the fine roots of AM species Cunninghamia lanceolata and the EM species Castanopsis carlesii might have the same foraging trend to N and P addition, and morphological traits of AM species Cunninghamia lanceolata fine roots were more sensitive to P addition than EM species Castanopsis carlesii.

Plant Systematics and Biogeography

• Primulina liangshengyui (Gesneriaceae), a New Species from Guangxi, China with Supplementary Description of Floral Morphology of P. minutimaculata

  CHEN Diya, LI Ruifeng, DING Li

  2024,32(5):629-636, DOI: 10.11926/jtsb.4820

  Abstract: (24) HTML (28) PDF (37.43 MKB)(2119)

  Abstract:

  Primulina liangshengyui R. F. Li & L. Ding, a new species of Gesneriaceae from the limestone of Guangxi, China is described and illustrated. It resembles P. minutimaculata (D. Fang & W. T. Wang) Yin Z. Wang, but can be easily distinguished by a combination of characters, especially in its leaf blades both sides very short white strigillose; bracts adaxially glabrous, abaxially densely white strigillose; calyx lobes both sides densely glandular puberulent; corolla tube purple and gradually fading to white at the base; filaments glandular puberulent. There is only one population with less 500 mature individuals at the type locality. Thus, this species was provisionally assessed as Vulnerable Endangered [VU D2] by using IUCN criteria. Further, supplemented and described of floral morphology of P. minutimaculata is also appended, based on plenty of specimens with flowers and field works, and especially according to living plant in bloom.

• Discussion on the Taxonomic Status of Epipogium sessanum

  HUANG Jing, SUN Mei, LIU Yue, MEI Li, TIAN Huaizhen

  2024,32(5):637-642, DOI: 10.11926/jtsb.4824

  Abstract: (27) HTML (29) PDF (2.40 MKB)(2124)

  Abstract:

  The taxonomic status of Epipogium sessanum was discussed. By field observations and a critical survey of the literature and specimens in herbaria, it was found that E. sessanum and E. roseum had obvious differences in the flower. It is suggested E. sessanum to be reinstated as a distinct species, and a new Chinese name “zang nan hu she lan” is proposed.

Plant Physiology and Molecular Biology

• Effect of Phosphorus on Cluster Bud Growth in Proliferating Seedlings of Acacia melanoxylon

  BAI Xiaogang, MO Yingxin, TU Panfeng, ZENG Bingshan, HU Bing

  2024,32(5):643-650, DOI: 10.11926/jtsb.4822

  Abstract: (33) HTML (36) PDF (2.50 MKB)(2111)

  Abstract:

  To understand the effect of phosphorus on the induction and growth of cluster buds in the proliferating seedlings of Acacia melanoxylon, the tissue-cultured seedlings of clone SR17 as materials was inoculated proliferation medium containing 0.9, 1.2, 1.6, 2.0, 2.4, and 2.7 mmol/L KH₂PO₄, respectively, and the growth and development indexes of cluster buds were investigated after for 1 and 2 cycles of subgeneration, respectively. The results showed that 2.0–2.7 mmol/L KH₂PO₄ promoted the rapid growth of height, number of cluster bud and base callus of proliferating seedlings, among which 2.4 mmol/L KH₂PO₄ had the best effect, and the accumulation of biomass and chlorophyll content was higher. With the enhance of phosphorus content in the medium, phosphorus and potassium contents in the cluster buds significantly increased, while nitrogen content had no significant change. Pearson correlation analysis revealed that the phosphorus level had significantly positive correlation with height, number, base callus, fresh weight, dry weight, tissue water content, and contents of total chlorophyll, chlorophyll a, chlorophyll b, phosphorus, potassium of bud in 1 and 2 cycles of subgeneration. Therefore, phosphorus plays an essential regulatory role in the growth and development of cluster buds of A. melanoxylon.

• Effects of Simulated Eutrophic Waterlogging and Post-waterlogging Drought on the Eco-physiological Characteristics of Dalbergia odorifera

  LI Dadong, WANG Haibo, YANG Fan, MIAO Lingfeng, ZHANG Juan, GUO Luyao, XIANG Lishan

  2024,32(5):651-659, DOI: 10.11926/jtsb.4816

  Abstract: (35) HTML (31) PDF (734.49 KKB)(2120)

  Abstract:

  In order to explore the effects of eutrophic waterlogging and post-waterlogging drought on the tolerance of terrestrial woody plants, the control (CK), nutrient (H), waterlogging (W) and eutrophication waterlogging (WH) treatments were set in the first stage. In the second stage, control-drought (CKD), nutrient- drought (HD), watering-drought (WD) and eutrophic watering-drought (WHD) treatments were set up to study the effects of different water and nutrient treatments on the growth and physiology of one-year-old Dalbergia odorifera seedlings. The results showed that D. odorifera had high survival under all treatments; the seedlings exhibited the highest biomass, net photosynthetic rate and total chlorophyll concentration under H treatment. In contrast, both W and WH treatments significantly reduced stem height increment, total biomass, net photo- synthetic rate, stomatal conductance, total chlorophyll content and leaf water potential, but significantly increased the activities of superoxide dismutase (SOD), peroxidase (POD), and contents of H₂O₂ and soluble protein. Notably, the impact of W stress was more pronounced than that of WH stress. Compared with the first stage, the biomass increment decreased significantly under both of CKD and HD treatments, but which showed the opposite trend under WD and WHD. Furthermore, compared with CKD and HD treatments, net photosynthetic rate significantly increased, while SOD activity and H₂O₂ content decreased under WD and WHD. Moreover, compared with WD, biomass increment, total chlorophyll content and POD activity increased under WHD, but Car content decreased. Therefore, it was demonstrated that nutrient supply could alleviate the negative effects of waterlogging on seedling growth of D. odorifera to a certain extent, and early waterlogging, especially eutro- phication waterlogging, was conducive to biomass accumulation of seedlings under later drought condition.

Phytochemistry and Chemical Biolgy

• Chemical Constituents from Petroleum Ether Fractions of Ainsliaea glabra and Antibacterial Activities

  JIANG Guodong, ZHANG Senyu, LIU Yafeng, AN Zhenyu, WU Xiuli, WANG Fang, JIANG Zhibo, MA Xiaoli, NAN Zedong

  2024,32(5):660-666, DOI: 10.11926/jtsb.4821

  Abstract: (25) HTML (30) PDF (578.13 KKB)(2121)

  Abstract:

  In order to understand the chemical composition of Ainsliaea glabra, ten compounds were isolated from petroleum ether extraction fraction of 90% ethanol extract by silica gel, sephadex LH-20 gel column chromatography, semi-preparative high-performance liquid chromatography. Based on spectral data, their structures were identified as pinerenol diisovalerate (1), 3β-hydroxy-11α,13-dihydro-costunolide (2), matricarin (3), taraxasteryl acetate (4), betulyl alcohol (5), 27-hydroxyolean-12-en-28-oic acid (6), morolic acid acetate (7), (-)-loliolide (8), butyl benzoate (9), and E-phytenal (10). Compound 1 was a new structural compound. Compounds 3 and 7 were isolated from Ainsliaea genus for the first time. Compounds 2-7 and 9 were first reported in the A. glabra. The results of antibacterial activity test showed that compounds 1, 2 and 8 had certain inhibitory effects on Staphylococcus aureus with MIC values of 31.2, 25.0, and 21.8 μg/mL, and compounds 1 and 3 had potential inhibitory effect on Candida albicans with MIC values of 1.9 and 3.9 μg/mL, respectively.

• Active Constituent Analysis in Six Camellia sect. Chrysantha Leaves

  WU Fan, XIA Lang, XIE Xin, ZHANG Yue

  2024,32(5):667-674, DOI: 10.11926/jtsb.4804

  Abstract: (55) HTML (31) PDF (2.41 MKB)(2128)

  Abstract:

  In order to understand the mineral element composition and active component, the contents of seven mineral elements (K, Mg, Ca, Na, Fe, Zn, Se), total phenol, total flavone, total ascorbic acid and soluble sugar, tea polyphenol, caffeine and amino acid in the leaves of six Camellia species, such as C. achrysantha, C. long- zhouensis, C. ptilosperma, C. limonia, C. chuongtsoensis and C. nitidissima, were determined. Based on the contents of active component, principal component analysis and cluster analysis were studied. The results showed that the contents of K, Mg, and Na in C. limonia were the highest, which were 18.70, 2.67, and 1.01 g/kg, respectively. The Ca content in C. achrysantha was the highest (42.99 g/kg), the Fe content in C. chuongtsoensis was the highest (334.14 mg/kg), the Zn content in C. ptilosperma was the highest with 22.46 mg/kg; the Se content in C. long-zhouensis was the highest with 0.47 mg/kg. There were significant differences in seven mineral elements in leaves among different species (P<0.05). A total of 15 amino acids were detected in 6 Camellia species, only asparagine, tryptophan, threonine, cysteine and glutamine were missing. The active ingredient contents in 6 Camellia species showed the trend of soluble sugar>total phenol>total flavone>total ascorbic acid, and those of C. ptilosperma were the highest. The content of tea polyphenol in C. achrysantha was the highest. The caffeine content in 6 Camellia species was low, but not detected in C. achrysantha and C. chuongtsoensis. The principal component analysis showed that the cumulative contribution rate of PC1, PC2 and PC3 reached 87.439%, and based on comprehensive score of component content, C. limonia was the best. The cluster analysis based on active component content showed that the difference between C. limonia and other species was large, and that between C. achrysantha and C. ptilosperma, C. chuongtsoensis and C. nitidissima was small. Therefore, these would provide theoretical basis for the development and utilization of Camellia sect. Chrysantha.

Research Progress

• Advances in Phylogenetic and Taxonomic Studies of the Genus Pogostemon Desf. (Lamiaceae)

  TAN Gan, YAO Gang

  2024,32(5):675-684, DOI: 10.11926/jtsb.4818

  Abstract: (32) HTML (28) PDF (23.07 MKB)(2130)

  Abstract:

  Pogostemon Desf. consists of about 80 species and is the largest genus in the tribe Pogostemoneae, subfamily Lamioideae, family Lamiaceae. Pogostemon cablin, which belongs to the subgenus Pogostemon, is the representative species of the genus due to its secondary metabolite (patchouli oil) widely used in cosmetics and pharmaceutical industry. The presence of exserted stamens bearing moniliform hairs is an important character to distinguish the genus from all other members circumscribed in the same tribe. The circumscription of the genus has been changed in large extent based on the consideration of results from phylogenetic analyses, and a new infrageneric classification system of the genus was also provided recently based on evidence from molecular and morphological data. Here in, advances in phylogenetic and taxonomic studies of Pogostemon were given. Moreover, some useful recommendations for further phylogenetic and taxonomic studies on the genus were provided, which would be important for further investigation and evaluation of plant resources of the genus. Twenty-nine species and two varieties in the genus were recorded in China, and a key to these taxa was provided.

• Research Status and Perspectives of Hopea hainanensis, a Wild Plant with Extremely Small Populations

  LI Yanpeng, XU Han, CHEN Jie, LEI Jie, LUO Wen

  2024,32(5):685-694, DOI: 10.11926/jtsb.4801

  Abstract: (33) HTML (31) PDF (590.75 KKB)(2115)

  Abstract:

  Because of the narrow distribution range, few individuals, and imperfect endangered relief technology, the wild plants with extremely small populations (WPESP) are facing the risk of extinction at any time, and need to be saved. Due to the differences in biological characteristics and endangered mechanisms of wild plants with very small populations, basic research on specific species is helpful to provide scientific basis for their conservation and population rejuvenation. Hopea hainanensis used to be the key tree species and characteristic species in the tropical rain forest of Hainan, but under the influence of human activities and regeneration difficulties, the population of H. hainanensis has decreased sharply. Although more research work has been carried out, problems such as unclear resource status, lack of mechanism research, unclear future research priorities, and failure of scientific research to effectively guide conservation practice are still prominent, and long-term, in-depth and systematic research needs to be strengthened. Based on a comprehensive search of relevant literature, the research progress of H. hainanensis was reviewed from the aspects of biological characteristics, natural distribution area, wild population size, adaptability, in situ and ex situ conservation, artificial breeding techniques and potential endangered mechanisms, and discussed the existing problems and future research priorities in the conservation of wild plants with very small populations. Based on the current research status, specific suggestions are put forward to provide reference for the protection and rescue of slope barrier.

Volume 32,2024 Issue 5

Volume 32,2024 Issue 5

Plant Ecology and Natural resource Management

• Current Situation and Evaluation of Landscape Restoration of Landfill in Big Taipa Hill, Macau

  WANG Lin, YI Qi-fei, SONG Xian-li, GUO Fei-li, LU Zhen-hua, XING Fu-wu

  2014,22(6):558-566, DOI: 10.11926/j.issn.1005-3395.2014.06.003

  Abstract: (2496) HTML (0) PDF (1.13 MKB)(71364)

  Abstract:

  In order to understand the landscape restoration of landfill in Big Taipa Hill, Macau, the species composition, vertical structure, floristic components, and community appearance were investigated by using sampling method. The results showed that there were 83 vascular plants in 12 plots of 1200 m². The floristic composition of the community was dominated by Pantropical genera, account for 38.98%. The community appearance was determined by the micro- and meso-phanerophyte with herbaceous, simple and micromonophylls. The indexes of the landfill community were similar to that of nature plant community, indicated that the restoration of landfill was good. Based on the current situation of landfill, some preferred species for landscape restoration were selected, and suggestions were given for improving landscape. It was recommended that the landscape restoration should be carried out in stages. To get faster and better landscape restoration, proper manual intervention should be taken at different stages of community succession.

Plant Systematics and Biogeography

• Anoectochilus lylei Rolfe ex Downie (Orchidaceae), A New Record from Yunnan, China

  HU Chao, TIAN Huai-zhen, DONG Quan-ying

  2012,20(6):602-604, DOI: 10.3969/j.issn.1005-3395.2012.06.011

  Abstract: (3214) HTML (0) PDF (10.08 MKB)(20473)

  Abstract:

  Anoectochilus lylei Rolfe ex Downie is newly reported to Chinese flora. The detailed morphological descriptions and photoes are also provided. This species is characterized by the white “Y”-shaped lips, epichiles lobes linear-oblong, mesochile with 1-3 short irregular denticles and the spur with 2 peltate calli inside.

• SEED VIGOUR OF BRASSICA ALBOGLABRA BAILEY UNDER DIFFERENT STORAGE CONDITION AND ULTRA-DRY TREATMENT

  Wu Xiaozhen, Fu Jiarui

  1997,5(3):69-73, DOI: 10.3969/j.issn.1005-3395.1997.3.015

  Abstract: (1062) HTML (0) PDF (0.00 ByteKB)(19050)

  Abstract:

Research Progress

• Review of Nitrogen Cycle in Plant-soil System Based on Application of ¹⁵N Tracer Technique

  WANG Qing-bing, ZHANG Jian-feng, CHEN Guang-cai

  2013,21(5):479-488, DOI: 10.396/j.issn.1005-3395.2013.05.016

  Abstract: (2393) HTML (0) PDF (766.89 KKB)(17454)

  Abstract:

  The isotope tracer technology is a kind of method by adding exogenous elements or substance as a tracer co-operating in organism completely, which used to indicate the change process of elements or substance in organism. Nitrogen stable isotope tracer technology can reveal essentially the mechanism of ecological process, so it becomes one of important tools for ecology research. In order to understand the advances in application of nitrogen stable isotope technique in nitrogen cycle of plant-soil system, nitrogen cycle based on ¹⁵N tracer technique was reviewed. The nitrogen stable isotope technique in solving ecology problems was prospected and some existing shortcomings are also discussed.

Plant Ecology and Natural resource Management

• Nutrient Characteristics in Incident Rainfall, Throughfall, and Stemflow in the Monsoon Evergreen Broad-leaved Forest at Dinghushan

  ZHANG Na, QIAO Yu-na, LIU Xing-zhao, CHU Guo-wei, ZHANG De-qiang, YAN Jun-hua

  2010,18(5):502-510, DOI: 10.3969/j.issn.1005-3395.2010.05.006

  Abstract: (3416) HTML (0) PDF (22.61 MKB)(17159)

  Abstract:

  The characteristics of five nutrient elements (K, Ca, Mg, N and P) in precipitation, throughfall and stemflow were studied in an monsoon broad-leaved evergreen forest at Dinghushan, Guangdong Province. Based on the precipitation allocation principles, the variation and input characteristics of these nutrients were calculated. The results showed that the contents of nutrient elements were in order as rainfall < throughfall < stemfall, in which the contents of K+ and total nitrogen (TN) were the highest, and those of total phosphorus (TP), HPO42- and total organic phosphorus (TOP) were the lowest. The biggest variation coefficient (Cv) of ion contents in precipitation was 1.282 for total organic nitrogen (TON), and the smallest was 0.502 for NO3-, while those in throughfall were 2.357 for TOP and 0.621 for TN, respectively. There were no significant relation between tree species and the contents of element (P>0.05). The annual inputs of nutrient elements from throughfall and stemflow into the soil were in the order as: TN>K+> Ca2+>Mg2+>TP. The annual input of Ca2+ from throughfall and stemflow were higher than that from litterfall decomposition. It concluded that rainfall was an important factor in transferring nutrient elements from forest canopy to soils.

Plant Systematics and Biogeography

• Taxonomic Studies on the Genus Delphinium (Ranunculaceae) from China (Ⅲ)：The Identities of D. pseudothibeticum, D. motingshanicum and D. caeruleum var. crassicalcaratum

  YUAN Qiong, YANG Qin-er

  2014,22(4):323-334, DOI: 10.3969/j.issn.1005-3395.2014.04.001

  Abstract: (2589) HTML (0) PDF (33.77 MKB)(16377)

  Abstract:

  Both herbarium and field observations have shown that Delphinium pseudothibeticum W. T. Wang & M. J. Warnock described from Mo-ting (= Mao-ding) Shan, Deqen, northwestern Yunnan, China, is identical with D. ceratophorum Franch. var. brevicorniculatum W. T. Wang, and that both D. motingshanicum W. T. Wang & M. J. Warnock and D. caeruleum Jacq. ex Camb. var. crassicalcaratum W. T. Wang & M. J. Warnock, also described from Mo-ting Shan, are identical with D. beesianum W. W. Smith (D. motingshanicum is no more than a gigantic form of D. beesianum). Delphinum ceratophorum var. brevicorniculatum is currently known only from Deqen, while D. beesianum is a very common species in northwestern Yunnan. Delphinium pseudothibeticum, D. motingshanicum and D. caeruleum var. crassicalcaratum are all synonymized.

• Progress in Studies on Plant Responses to Elevated CO₂

  Zhi-Ying Ou， Chang-Lian Peng^*

  2003,11(2):190-196, DOI: 10.3969/j.issn.1005-3395.2003.2.021

  Abstract: (3005) HTML (0) PDF (0.00 ByteKB)(16310)

  Abstract:

  Recent studies on plant responses to elevated CO₂ is reviewed. Increase in atmospheric CO₂ concentration affects not only global climate but also plant morphology, water use, protein synthesis, photosynthesis, resistance, growth, biomass and so on. Elevated CO₂ has effects of promoting the growth of plant roots and seedlings, making the leaf thick, lowering the stomatal density, stomatal conductance and transpiration, enhancing the water use efficiency, crop yield and biomass, intensifying the biosynthesis of ethylene and antioxygen competence. High CO₂ concentration together with other environmental factors affect plants. Plants with different photosynthetic pathways (C₃, C₄ and CAM) and different vegetation types (natural and artificial vegetation) exhibite different responses to high CO₂ concentration. Short-term exposure to elevated CO₂ enhances photosynthesis while long-term inhibits. Experimental conditions such as air pressure, temperature, water availability, nutrition status, oxygen, light intensity even growth space also have great effect on the results of the experiments.

Plant Systematics and Biogeography

• Dynamic Change of Stomatal Density and Stomatal Index on the Flower during Canna generalis Bailey Development

  MA San-mei, LIN Wen-le, YANG Wei-dong

  2010,18(5):497-501, DOI: 10.3969/j.issn.1005-3395.2010.05.005

  Abstract: (4048) HTML (0) PDF (10.82 MKB)(15717)

  Abstract:

  The changes in stomatal distribution, density and stomatal index of Canna generalis Bailey flower at different development stages were studied. The results showed that stomata distributed in upper and lower epidermis of petal, bract and calyx. Stomatal density and stomatal index on upper epidermis of petal, bract and calyx were smaller than those on lower surface. With the development of flower, the stomatal density and stomatal index on the petal, bract and calyx increased firstly and then decreased.

• Notes on the Geographical Distribution of Ligularia przewalskii and Critical Comments on Some Problems Apparent in Floristic Works of China

  YANG Qin-er

  2014,22(2):107-120, DOI: 10.3969/j.issn.1005-3395.2014.02.001

  Abstract: (2629) HTML (0) PDF (19.65 MKB)(12398)

  Abstract:

  We point out that the inclusion of east China's Jiangsu Province in the distribution of Ligularia przewalskii, a species actually occurring only in Gansu, Qinghai, Nei Mongol (Inner Mongolia), Ningxia, Shaanxi, Shanxi, and Sichuan, most likely is a result of misinterpreting "Kansu (= Gansu)" as "Kiangsu (= Jiangsu)". This inadvertent error first appeared in Flora Reipublicae Popularis Sinicae 77(1), and was widely perpetuated in subsequent publications. Critical comments are made on some problems apparent in floristic works (particularly regional Floras at provincial level) of China.

• A REVIEW OF THE STUDIES ON ATPase IN PLANT CELLULAR MEMBRANES IN RELATION TO PHYSIOLOGICAL PROCESS AT LOW TEMPERATURE

  Li Meiru, Liu Hongxian, Wang Yirou

  1997,5(3):74-82, DOI: 10.3969/j.issn.1005-3395.1997.3.016

  Abstract: (1137) HTML (0) PDF (0.00 ByteKB)(9735)

  Abstract:

Research Progress

• Advances in Chemical Constituents and Biological Activities from Aquilaria sinensis

  LI Wei, MEI Wen-li, ZUO Wen-jian, WANG Hao, DAI Hao-fu

  2014,22(2):201-212, DOI: 10.3969/j.issn.1005-3395.2014.02.015

  Abstract: (2773) HTML (0) PDF (1.80 MKB)(5603)

  Abstract:

  The chemical constituents of Aquilaria sinensis mainly include flavonoids, benzophenones, lignans, phenylpropanoids, terpenoids, alkaloids, steroids, phenolic compounds. Some of them have anti-tumor, antibacterial, anti-inflammatory, analgesic, and laxative activities. The chemical constituents isolated from A. sinensis were reviewed, and the biological activities of some compounds were also introduced. These would provide scientific basis for development and utilization of A. sinensis.

• A Survey of the Studies on the Resources of Jatropha curcas

  LIN Juan， ZHOU Xuan-wei， TANG Ke-xuan， CHEN Fang

  2004,12(3):285-290, DOI: 10.3969/j.issn.1005-3395.2004.3.018

  Abstract: (2713) HTML (0) PDF (0.00 ByteKB)(5396)

  Abstract:

  This paper briefly reviews the studies of medicinal plant Jatropha curcas on the following aspects: morphology and habitat; effective components including terpens, flavons, fats, coumarins, sterols, etc.; toxicity and pharmacological activities; medicinal use; and application prospects.

• Primary Vasculature of the Root—Stem Transitional Zone in Robinia pseudoacacia L. Seedlings

  HUANG Jun-zhe, GU Song^*，HUANG Ya-nan, ZHENG Qiang, TIAN Jun-ying，JIANG Sha^*

  2007,15(3):219-223, DOI: 10.3969/j.issn.1005-3395.2007.3.006

  Abstract: (3001) HTML (0) PDF (0.00 ByteKB)(5285)

  Abstract:

  The development of primary vascular tissues of root-stem transitional region was studied in Robinia pseudoacacia L. seedlings of 1-12 days using sequential paraffin section. The primary vascular tissues of roots are connected with the cotyledons via the hypocotyl. The results support the viewpoints of Esau et al. that the whole seedling of Robinia pseudoacacia L. is divided into two systems of the root-hypocotyl-cotyledon and the epicotyl, and there is only a simple linkage between the vascular tissues of stem and the hypocotyl without any transition.

• The Role of Cytokinins in Retarding of Senescence in Plants

  YANG Xiao-hong, CHEN Xiao-yang, LIU Ke-feng

  2006,14(3):256-262, DOI: 10.3969/j.issn.1005-3395.2006.3.016

  Abstract: (2439) HTML (0) PDF (0.00 ByteKB)(5156)

  Abstract:

  Cytokinins are important plant hormones and can delay plant senescence, In this review three approaches in study of the effects of cytokinins on plant senecence are presented： the analysis of endogenous cytokinins during senescence； the application of exogenous cytokinins； and the research on transgenic plants with cytokinin biosynthesis or biodegradation genes. The mechanisms of cytokinins in retarding of senescence was discussed in relation to saccharides, lipid oxidation, as well as to other hormones.

• Medicinal Plants of Bulbophyllum Species in China

  YI Qi-fei， XING Fu-wu^*， HUANG Xiang-xu， CHEN Hong-feng， WANG Fa-guo

  2005,13(1):65-69, DOI: 10.3969/j.issn.1005-3395.2005.1.013

  Abstract: (2922) HTML (0) PDF (0.00 ByteKB)(5139)

  Abstract:

  Fifteen medicinal Bulbophyllum species in China are presented. The local names, distribution, habitat, and folk medicinal uses are given. Bulbophyllum species used as folk herbs are mostly distributed in south and south-west China, in particular, in Yunnan Province. Some species such as B. kwangtungense, B. odoratissimum and B. ambrosia are common in Guangdong and Guangxi. Some suggestions for the conservation and reasonableutilization of the plant resources are made.

• Advances in the Studies on Postharvest Physiology and Fresh-keeping Methods of Annona Fruits

  LI Wen， CHEN Wei-xin^*

  2004,12(3):280-284, DOI: 10.3969/j.issn.1005-3395.2004.3.017

  Abstract: (2091) HTML (0) PDF (0.00 ByteKB)(5081)

  Abstract:

  The development of annona fruit production is restricted due to the difficulty in fresh-keeping of the fruits during storage and transportation. This paper reviews postharvest physiology of annonas, including respiration and ethylene production, relevant enzymes, fruit softening, fruit browning, and postharvest pathology.

• A Review of the Pathway of Ethylene Biosynthesis and the Relevant Genetic Engineering

  CHEN Xin-jian， LIU Guo-shun， CHEN Zhan-kuan， ZHI Yu-bao， YI Ming-lin， LIU Hong-xian

  2002,10(1):83-98, DOI: 10.3969/j.issn.1005-3395.2002.1.018

  Abstract: (1806) HTML (0) PDF (0.00 ByteKB)(4960)

  Abstract:

  This review contents a brief retrospection of physiological functions of plant hormone，ethylene The progresses concerned three important enzymes：SAMS，ACS and ACO in ethylene biosyn-thetic pathway are highlighted The possible methods to control endogenous ethylene’s productionin fruits or flowem by genetic engineering are outlined．

• Identification of Chemical Constituents from Polygonum chinense Linn.

  XIE Xian-qiang, WU Ping, LIN Li-dong, LIU Mei-fang, WEI Xiao-yi*

  2007,15(5):450-454, DOI: 10.3969/j.issn.1005-3395.2007.5.015

  Abstract: (3271) HTML (0) PDF (0.00 ByteKB)(4804)

  Abstract:

  Twelve chemical constituents were isolated from Polygonum chinense Linn. On the bases of spectral data, they were identified as syringic acid (1), apigenin (2), 3,3′-di-O-methylellagic acid (3), gallic acid (4), protocatechuic acid (5), isorhamnetin (6), caffeic acid (7), quercetin (8), luteolin (9), gallicin (10), avicularin (quercetin-3-O-α-L-arabinofuranoside)(11) and 3,4,8,9,10-pentahydroxy -6H-dibenzo [b,d]pyran-6-one (12). All these compounds except 4 and 8 were reported from the species for the first time.

Plant Ecology and Natural resource Management

• Studies on Parasitic Relationship between Santalum album L. and Leguminous Plants

  CHEN Rong, ZHANG Xin-hua, MA Guo-hua*

  2014,22(1):53-60, DOI: 10.3969/j.issn.1005-3395.2014.01.009

  Abstract: (2436) HTML (0) PDF (1.39 MKB)(4543)

  Abstract:

  In order to understand the parasitic relationship between Santalum album L. and leguminous plants, eleven leguminous species were planted with S. album. The leaf photosynthetic characters, height and base diameter of S. album and its haustoria number and size were studied. The results showed that number and size of haustoria, height and base diameter of S. album had significant differences among different hosts. The excellent hosts for S. album were Calliandra haematocephala, Caesalpinia sappan, Acacia confusa and Erythrina corallodendron, and Acacia mangium, Dalbergia odorifera and Cassia surattensis were just general hosts, while Delonix regia, Ormosia pinnata, Leucaena leucocephala and Bauhinia blakeana were unsuitable hosts. Meanwhile, the height and diameter, leaf photosynthesis characters of S. album had close relation with number of haustoria parasitized on roots of leguminous plants. So, the plantation of S. album should choose appropriate host plant.

Plant Physiology and Molecular Biology

• IQM3 Regulated Main Root Length of Arabidopsis thaliana at Downstream of CO under Long Sunshine

  YU Jieyu, LI Shuqiang, WANG Yunhui, FAN Tian, Lü Tianxiao, ZHOU Yuping, TIAN Chang'en

  2024,32(4):451-457, DOI: 10.11926/jtsb.4790

  Abstract: (95) HTML (3817) PDF (8.03 MKB)(4507)

  Abstract:

  In Arabidopsis thaliana, IQM family is a calmodulin-binding protein family containing IQ motifs, IQM3 mutation can increase main root length, CO (CONSTANS) is an important member of photoperiodic floral regulation pathway, CO mutation can shorten taproot length. The genetic relationship between IQM3 and CO gene was studied by constructing double mutants of IQM3 and CO. The results showed that the first exon in co-12 sequence of the new CO mutant was missing 9 base ACTTGCTAG, which contains the restriction endonucrenase BfaI restriction site CTAG. A molecular marker was established to identify the mutant. When the cross-coding PCR product was cleaved by BfaI enzyme, four fragments were obtained from wild-type Col because it had three sites, three fragments were obtained from co-12 because it had only two sites, and five fragments were obtained from CO/co-12 heterozygote. Under long sunshine, the main root length of co-12 was shorter than that of wild type Col, and that of iqm3-2 was longer than that of wild type, while the phenotype of double mutant co-12 iqm3-2 was more favorable to iqm3-2. Therefore, IQM3 acts downstream of CO to regulate main root length under long day in Arabidopsis.