• Volume 4,Issue 4,1996 Table of Contents
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    • TOWARDS A REVISION OF THE MYRSINACEAE OF INDOCHINA

      1996, 4(4):1-15. DOI: 10.3969/j.issn.1005-3395.1996.4.001

      Abstract (2240) HTML (0) PDF 0.00 Byte (1909) Comment (0) Favorites

      Abstract:The Myrsinaceae is a richly represented family in tropical and subtropical Asia.In Indochina, Pitard (1930) revised the family for Lecomte's Flore Generale de L' Indochine and recongnized 109 species in 6 genera, but since then there has been little work on this interesting group from this area. This paper is a precursor to the account of the Myrsinaceae in the "Flore du Cambodge du Laos et du Vietnam" Thirty new species and ten new varieties are described in Ardisia(A. aciphylla var. touranensis, A.applanata, A. asymmetrica, A. baviensis, A. caloneura, A. chinensis var. stenophylla, A.clemensii, A. crotonifolia, A. dumicola, A. fasciculifolia, A. filipendula, A. gracilenta, A.illicioides, A. interjacens, A. lamdongensis, A. lucidula, A. macrosepala var. schmidii, A.merrillii var. rosea, A. nhatrangensis, A. nhatrangensis var. glaucescens, A. nhatrangensis var. neurophylla, A. paradoxa, A. pitardii, A. prolifera, A. prunifolia, A. quangnamensis,A. rapaneifolia, A. ravida, A. rhodochroa, A. rubescens var. oblongifolia, A. rubescens var.puberula, A. silvestris var. appressa, A. suboppositifolia, A. vidalii, A. vidalii var.orbicularis,. A. vietnamensis), Embelia(E. cuneata) and Maesa (M. cambodiana, M. kerrii, M.laevis) Six new combinations are proposed in Ardisia (A. crenata subsp. crassinervosa, A.crenata subsp. mouretii, A. obtusa var. montana, A. perpendicularis var. balansana, A.prionata var. linearifolia, A. quinquegona var. salicifolia). The new taxa and new combinations are alphabetically arranged under the appropriate genus.

    • THREE SPECIES OF BAUHINIA L.(LEGUMINOSAE) MW TO CHINA

      1996, 4(4):16-17. DOI: 10.3969/j.issn.1005-3395.1996.4.002

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      Abstract:本文报道了三种羊蹄甲属植物在中国的新分布记录,即产自广西的中越羊蹄甲、耐看羊蹄甲和产自云南的圆叶羊蹄甲.

    • TWO POLYTHETIC-AGGLOMERATIVE CLUSTERING STRATEGIES

      1996, 4(4):18-23. DOI: 10.3969/j.issn.1005-3395.1996.4.003

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      Abstract:Based on extended Lance and Williams' model, two polythetic agglomerative dustering strategies, i.e. the Average distance of new cluster and the Weighted average distance,are introduced in this paper. As an example, vegetation data of shrub, Vitex negundo var.heterophylla, collected from Zhongtiao mountains in Shanxi Province were analysed with these two new methods. The Results illustrate that the two methods are effective and successful in describing the relationships between vegetation clusters. They have some advantages compared with fuzzy equivalence clustering and group averaging, and are partically suitable for vegetation classfication at association level.

    • ASSESSMENT OF THE WHOLE SHOOT LEAF AREA ON BASIS OF SINGLE LEAF AREA ESTIMATES OF SALIX VIMINALIS DURING THE FIRST GROWING SEASON

      1996, 4(4):24-33. DOI: 10.3969/j.issn.1005-3395.1996.4.004

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      Abstract:Linear (Y=b×X) and nonlinear (y=b ×Xc) relations of leaf area (AL) With leaf length(L), leaf width(W), L2, W2 and LW were developed in Salix viminalis at the single leaf level, and used to assess leaf area at whole shoot level. Most relationships were significantly nonlinear, but both types exhibited good fits as indicated by r2 which was 0.941 (W)-0.998(LW) for the linear, and 0.982(W,W2)-0.998(LW) for the nonlinear equations.The nonlinear relationship between AL and LW was different for leaves from proleptic and sylleptic shoots, and varied with the growth stage. The nonlinear regression of AL on leaf dry weight (WL) varied with the leaf type and vertical position on shoot. Differences in leaf shape could be modelled successfully by geometric shapes, and leaf area could be obtained adequately with the hasal part of the leaf as a parabola and the distal part as an ellipse. The nonlinear equation performed better than the linear when used to predict the leaf area of the proleptic shoot (AP) with its diameter (D), length (H), D2H, D times the number of leaves (NL·D) on it alone, and the inaccuracy of the estimates of AP by both equation types could be increased after the emergence of the sylleptic shoots. With the nonlinear equation,leaf area of the sylleptic shoot (AS) could be predicted by its number within the proleptic shoot times D (NSS·D) or by its dry weight (WS) alone. All the nonlinear relationships of AP, As on shoot characters for both shoot types varied with the sampling occasion.

    • EFFECTS OF FORESTATION BY KEEPING BROADLEAF TREES IN PLANTING CUNNINGHAMIA LANCEOLATA

      1996, 4(4):34-41. DOI: 10.3969/j.issn.1005-3395.1996.4.005

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      Abstract:The results of forestation with mixed broadleaf trees in planting Cunninghamia Ianceolata at Linduo Forest Farm in Guangxi Province showed that the total growing stocks in stands of 19-year-old and 30-year-old mixed forests were 184.78 and 515.58m3 hm(-2),respeCtively, which were by 23.0% and 21.8% higher than those of the same years old forests of pure Cunninghamia lanceolata, respectively. In mixed forests, the larger proportion of broadleaf species, the less became mean height, diameter and growing stocks of Cunninghamia lanceolata. The mean standing crop of litter on forest floor in mixed forests was 15.18t hm(-2), and the accumulation of nutrient elements (N, P, K, Ca, Mg) in the litter was 422.22kg hm(-2), being by 25.30% and 32.33% higher than those in pure Cunninghamia Ianceolata forest, respectively. The nutrient concentration of organic matter, total N,hydrolazable N, available P and rapidly available K in the soil, and soil porosity and water-holding capacity under mixed forest were higher than those under pure Cunninghamia Ianceolata forest.

    • A STUDY ON THE SOIL SEED BANKS AT THE DIFFERENT SUCCESSION STAGES OF SOUTH SUBTROPICAL FORESTS

      1996, 4(4):42-49. DOI: 10.3969/j.issn.1005-3395.1996.4.006

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      Abstract:Soil seed banks at four succession stages of southsubtropical forests (Pine forest,Pine and broad-leaf mixed forest, young monsoon evergreen broad-leaf forest and mature monsoon evergreen broad-leaf forest) in Dinghushan Nature Reserve, Guangdong Province,were investigated. Four quadrates (1m× 1m each) were measured three times (one was in dry, one in rainy season, another was before rainy season) for each stages, and three soil stratifications were sampled for each quadrate. The soil samples were set in laboratory under sufficient humidity and nature sunlight, and seed gendnation was noted daily. The results showed that the number of seeds in the soil seed banks decreased with the development of forest succession, but the species diversity increased. The number of seeds and the species diversity were both higher in rainy season than in dry season. The number of seeds in the stratification at 3-5cm depth was almost the highest as compared with other stratifications.According to a regression analysis, species composition in the soil seed banks was less related to that on the ground vegetation. The seeds of herb species were dominant, but a few dominant species of ground vegetation were not found in the soil seed banks because of the short life of some seeds and eating by animals. The germination of seeds in the soil seed banks was more closely related to temperature than to humidity. The roles of soil seed banks in forest succession were discussed.

    • EFFECTS OF PRO-LONG COATING ON ETHYLENE BIOSYNTHESIS AND OTHER RELEVANT PHYSIOLOGICAL CHANGES OF BANANA FRUIT DURING RIPENING

      1996, 4(4):50-59. DOI: 10.3969/j.issn.1005-3395.1996.4.007

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      Abstract:Coating banana (Musa acuminata Colla cv. Dwarf Cavendish) fruitS with 1.5%Pro-long delayed chlorophyll breakdown in peel, the decrease in firmness of the fruit and the increase in ethylene production of the whole fruit. During ripening, the accumulation of ACC content occurred in banana fruit treated with Pro-long. The beak in ACC content appeared a short time before that in ethylene production and in EFE activity. The peaks in ACC content and EFE activity of Pro-long treated fruits occurred 5 days later than that of the control. During ripening EFE activity in banana pulp discs treated with Pro-long was partially inhibited (from 19.45% to 40.51%). MACC contentS in banana fruit were at significant levels at the beginning, and then increased gradually with the development of ripening, but only slightly as compared With the marked increase in ACC levels. We suggest that effectS of Pro-long coating on banana fruit during ripening mainly due to affecting ethylene biosynthesis of the fruit through partially inhibiting EFE activity by reducing the supplement of oxygen, so as to delay the increase in ethylene production and so extend the ripening processes.

    • STUDIES ON PHOTOSYNTHETIC CHARACTERISTICS IN RICE HYBRID PROGENIES AND THEIR PARENTS I. CHLOROPHYLL CONTENT, CHLOROPHYLL-PROTEIN COMPLEX AND CHLOROPHYLL FLUORESCENCE KINETICS

      1996, 4(4):60-65. DOI: 10.3969/j.issn.1005-3395.1996.4.008

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      Abstract:Studies were made on photosynthetic rates, chlorophyll contents and chl a/b ratio,chlorophyll-protein complexes and chlorophyll induction fluorescence kinetics in two rice hybrid progenies in comparison with their parents. Two rice hybrids used were Qingyou 159(F1) and its parents Qing A(♀) and R159(♂), Guangyou No. 4(F1) and its parents Guang A(♀) and Qingliuai(♂). It was found that the photosynthetic rates of both hybrids were much higher than their parentS (18% heterobeltiosis, 26%-30% mid-parent heterosis). The chlorophyll contents in leaves of both hybrids were not much different from that of their parents and showed only a low mid-parent heterosis, while chl a/b ratios were lower in these two hybrids than in their parents. Statistical analysis showed no correlation between photosynthetic rates and chlorophyll contents, and the chl a/b ratio showed significant negative correlation with photosynthetic rate. Determination of the chlorophyll-protein complex content by SDS-electrophoresis followed by scanning of the electrophoretogram at 650nm and 675nm showed that the peak area of OD values in both hybrids were much higher than that of their respective parents with marked heterobeltiosis, and was significantly correlated with photosynthetic rate. Chlorophyll fluorescence induction kinetics studies showed that the characteristic parameters (Fv/Fo and Fv/Fm) in the hybrids were higher than those of their respective parents, indicating a higher photosystem Ⅱ activity and high primary photochemical efficiency of photosystem Ⅱ in these two rice hybrids than that in their respective parents.

    • PURIFICATION AND CHARACTERIZATION OF CYSTEINE ENDOPEPTIDASE IN COTYLEDONS OF GERMINATED PEANUT (ARACHIS HYPOGAEA L.) SEEDS

      1996, 4(4):66-73. DOI: 10.3969/j.issn.1005-3395.1996.4.009

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      Abstract:A cysteine endopeptidase (EC 3.4.22) was purified from cotyledons of germinated peanut seeds by buffer extraCtion, ammonium sulfate precipitation and succesive chromatagrafies on Sephadex G-100, DEAE-cellulose and DEAE-Sephedax A-50 columes. The overall purification and final recovery were 28.1 fold and 15.9%. This endopeptidase exists in two isoenzymic forms and SDS-PAGE revealed two polypeptides of 58 and 55KD. The optimumtemperature was 50℃ at 8.1 pH for degrating BAPNA (Benzoylarginine-p-nitroanilide) in Tris-HCl buffer. Inhibitor studies demonstrated that the endopeptidase belongs to the cysteine class of endopeptidase. This enzyme hydrolysed only 5-10% of arachin or coarachin Ⅰor 2s protein of ungerminated peanut seeds in vitro. The characterization of the endopeptidase was almost consistent with the peptide hydrolase in the study of Cameron and Mazelis who purificated from cotyledons of ungeednated peanut seeds.The results of present paper and our other studies suggested that the cysteine endopeptidase of peanut cotyledons which plays an important role during seed germination was synthesized during seed development and can only degrade the modified storge proteins.

    • EFFECTS OF CuZn-SUPEROXIDE DISMUTASE ON SEVERAL HYDROXYL RADICAL GENERATING SYSTEMS

      1996, 4(4):74-78. DOI: 10.3969/j.issn.1005-3395.1996.4.010

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      Abstract:The effects of CuZn-superoxide dismutase (SOD) on several hydroxyl radical generating systems were studied by using deoxyribose which reacted with hydroxyl radical to release thiobarbituric acid-reactivity compounds. When hydroxyl radical was formed in the Fe3+·O· H2O2 system, its formation was effectively inhibited by SOD, and just slightly inhibited by inactive SOD or bovine serum albudrin (BSA). In Fe(2+)· H2O2 or Cu(2+)· H2O2 system,the hydroxyl radical production was significantly inhibited by BSA. inactive SOD, or SOD which had similar effect to that of inactive SOD. The hydroxyl radical generation from Fe(2+)·O system could be effectively suppressed by inactive SOD or BSA, but not by the "active" SOD which had little effect on hydroxyl radical generation from this system. It is suggested that SOD could affect the hydroxyl radical formation through the following pathways: 1. scavenging superoxide to inhibit Haber-Weiss reaction, 2. chelating metal ions to decrease hydroxyl radical formation; 3. accelerating H2O2 accumulation to enhance hydroxyl radical formation from Fenton reaction.

    • ALLOZYME DIVERSITY IN POLYPLOID COMPLEX OF ARRHENATHERUM ELATIUS

      1996, 4(4):79-85. DOI: 10.3969/j.issn.1005-3395.1996.4.011

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      Abstract:A total of 27 populations of Arrhenatherum elatius collected from Europe and Mediterranean area were sampled and scored for electrophoretic variation at 3 loci.The level of genetic diversity was high in A. elatius and its four subspecies (He:0.3250.533). The total gene diversity in each subspecies was mainly maintained within populations.There were high gene flow estimates among populations (G(ST):0.035-0.1459). The tetraploid subspecies exhibited higher diversity than diploid subspecies. The result of UPGMA on Net's standard genetic distance showed that the tetraploid form was derived from the bulbiferous diploid plants.

    • GENE TRANSFORMATION AND TRANSGENIC PLANTS REGENERATION OF ANTIRRHINUM MAJUS WITH AGSOBACTERIUM TUMEFACIENS

      1996, 4(4):86-90. DOI: 10.3969/j.issn.1005-3395.1996.4.012

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      Abstract:In this paper, G418-resistant regenerated plants were obtained from hypocotyl segments which had cocultivated with Agrobacterium tumefaciens LBA4404 (p35SGUSINT) via adventitious bud regeneration. DNA/DNA dot hybridization and GUS histochendcal assay revealed that the foreign GUS gene from Agrobacterium tumefaciens LBA4404 had been integrated into genome of Antirrhinum mains and expressed in the cells.

    • RAPID AND EFFICIENT PURIFICATION OF DNA FROM PEANUT (ARACHIS HYPOGAEA)SEEDS

      1996, 4(4):91-93. DOI: 10.3969/j.issn.1005-3395.1996.4.013

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      Abstract:

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