Identification of Manihot esculenta JAZ Genes and Their Response Pattern to Different Stresses

Abstract:

Jasmonate ZIM-domain (JAZ) proteins play an important role in plant response to biological and abiotic stresses. There were 16 MeJAZ genes identified in Manihot esculenta by bioinformatics, which were unevenly distributed on 11 chromosomes. Among the 16 MeJAZ genes, 7 pairs of gene showed collinear relationship, all of which were fragment repeat genes, and the Ka/Ks of the 4 collinear genes with high similarity were less than 1, indicating that the JAZ family genes were selected for purification in evolution. Thirteen MeJAZ proteins contained ZIM, Jas and NT domains, and the other 3 MeJAZ proteins were missing NT domains. Thirteen MeJAZ genes contained complete UTR, intron and exon sequences, the other genes lacked the UTR region or intron sequences. The promoter region of MeJAZs contained multiple cis-acting elements responsing to abiotic stresses, ABA and SA. There were more MeJAZs with high expression in root tuber and flower, such as MeJAZ2/MeJAZ4/MeJAZ6/MeJAZ9/MeJAZ13, followed by stems. At the same time, most of MeJAZ genes responded to multiple abiotic stresses, drought induced the expression of more MeJAZ genes, and the MeJAZ16 gene was significantly induced by SA. Therefore, these would provide a reference for genetic improvement and selection of high-quality genes in Manihot esculenta.

Key words:Manihot esculenta;JAZ family gene;Tissue specific expression;Abiotic stress