Abstract:Annamocarya sinensis is a national Class Ⅱ key protected wild plant with extremely small populations.To elucidate the genetic diversity and variation of wild A. sinensis population in Guangxi can provide guidance for the development of genetic diversity conservation and management strategies. The genetic diversity and genetic variation of 4 wild populations (47 individuals) in Guangxi were analyzed by using 6 pairs of SSR primers with good stability and polymorphism. The results showed that the average number of observed alleles was 2.125, the average number of effective alleles was 1.581, the average Shannon information index was 0.477, the average observed heterozygosity was 0.310, the average expected heterozygosity was 0.286, and the average allele richness was 1.677. The level of genetic diversity of A. sinensis in Guangxi was low. There was a moderate genetic differentiation among populations (0.120), accounting for 20% among populations and 80% within population. Structure and UPGMA analysis showed that the 47 samples could be divided into three categories, which were mixed with each other and had rich genetic background. The genetic diversity of Yongfu County population in Guilin City was the highest (He=0.339, I=0.598), which should be regarded as the key conservation unit.
