Abstract:Paphiopedilum appletonianum is an endangered and class I protected orchid. In order to understand the status of its wild population and promote the protection of wild population, two large populations distributed in Nangaoling and Bawangling in Hainan were investigated in the field. The population distribution pattern was analyzed by point pattern method, and the population dynamic characteristics was analyzed through static life table, survival curve analysis, survival analysis, population reproduction strategy analysis and fertility table. The results showed that the distribution pattern of the two populations changed from aggregation to random and uniform distribution as the scale increased. The Bawangling population was mainly clustered at different scales, while the Nangaoling population was mainly random and even distributed. The age structure of the two populations was pyramid shape, and the survival curve was Deevey-II type, showing a decline trend with the increase of age class. There were two reproductive strategies: sexual reproduction and clone reproduction, and the asexual reproduction of Bawangling population was stronger, and the sexual reproduction of Nangaoling population was stronger. Therefore, Bawangling population was a growing population, while Nangaoling population was a slow negative increasing population.