Home > Archive>Volume 32, Issue 3, 2024 >417-426. DOI:10.11926/jtsb.4814
PDF HTML XML Export Cite reminder
Analysis of Agronomic Traits, Anatomical Structure, Photosynthetic Characters and Metabolomic Characteristics of Leaf Chlorosis Mutant ‘MT-1’ in Aristaloe aristata
Author:
  • Article
    • | |
  • Metrics
    • |
  • Reference [30]
    • |
  • Related [4]
    • | | |
  • Comments
    Abstract:

    In order to elucidate the mutation mechanism of leaf color mutation ofAristaloe aristata, the phenotypic characteristics, leaf ultrastructure, photosynthetic pigment content, chlorophyll fluorescence parameters and metabolomics between leaf color mutant ‘MT-1’ and normal leaf color germplasm ‘WT-18’ were compared. The results showed that compared with ‘WT-18’, the mutant ‘MT-1’ exhibited yellow strips from young leaves, and the plant type became smaller and the growth was slower. The number of stomata became less and small, and the shape changed from rectangle to square. The number of chloroplasts and internal lamella decreased, and the volume decreased obviously. The contents of photosynthetic pigments, including chlorophyll a, chlorophyll b and carotenoids, and chlorophyll fluorescence dynamic parameters (Fo, Fm, Fv/Fm, ФPSⅡ, qP, qN and ETR) decreased significantly. The metabolites of ‘MT-1’ and ‘WT-18’ were significantly different. The KEGG metabolic pathway analysis showed that most of the different metabolites were enriched in amino acid metabolism, glucose metabolism, lipid metabolism, nucleic acid metabolism, secondary metabolite biosynthesis, and other pathways. Therefore, the changes in chloroplast related genes might be an important cause of leaf chlorosis mutation.

    Reference
    [1] XIE Y F, XUN N, CAO Y Y, et al. Structural characterization and spectroscopic analysis of the aloin [J]. Spectro Spectral Anal, 2014, 32(2): 385-388. [谢云飞, 郇楠, 曹元元, 等. 芦荟中芦荟苷的结构表征及光谱分析 [J]. 光谱学与光谱分析, 2014, 32(2): 385-388. doi: 10.3964/j.issn.1000-05932014)02-0385-04.]
    [2] FU Y Z, HUANG K K, LIN B, et al. Preparation of C-TiO2 from fresh aloe extract and degradation of ofloxacin by visible light [J]. J China Univ Petroleum (Nat Sci), 2022, 46(5): 177-182. [付云芝, 黄康康, 林彬, 等. 鲜芦荟提取液中C-TiO2的制备及可见光下氧氟沙星的降解 [J]. 中国石油大学学报(自然科学版), 2022, 46(5): 177-182. doi: 10.3969/j.issn.1673-5005.2022.05.020.]
    [3] LI W P, YANG G X, CHENG Z Q, et al. Preparation and properties of polyvinylpyrrolidone/aloe composite nanofiber membrane [J]. J Textile Res, 2022, 43(8): 55-59. [李伟平, 杨桂霞, 程志强, 等. 聚乙烯吡咯烷酮/芦荟复合纳米纤维膜的制备及其性能 [J]. 纺织学报, 2022, 43 (8): 55-59. doi: 10.13475/j.fzxb.20210700605.]
    [4] CHU J H, JU R J, FAN Y H, et al. Protective effect and mechanism of aloe bark extract on acute alcoholic liver injury [J]. Sci Technol Food Ind, 2023, 44(1): 378-388. [褚佳豪, 居瑞军, 樊远红, 等. 芦荟皮提取物对急性酒精性肝损伤的保护作用与机制 [J]. 食品工业科技, 2023, 44(1): 378-388. doi: 10.13386/j.issn1002-0306.2022020064.]
    [5] WANG T T, LI Y, YANG L L, et al. In vivo dynamic detection of aloe polysaccharides using carbon fiber microelectrodes modified with gold nanoparticles [J]. Chem J Chin Univ, 2020, 41(1): 87-93. [王婷婷, 李元, 杨莉莉, 等. 碳纤维微电极负载金纳米粒子用于芦荟多糖的在体动态监测 [J]. 高等学校化学学报, 2020, 41(1): 87-93. doi: 10. 7503/cjcu20190422.]
    [6] YANG C, ZHANG Y Y, FANG Z Y, et al. Photosynthetic physiological characteristics and chloroplast ultrastructure of yellow leaf mutant YL-1 in cabbage [J]. Acta Hort Sin, 2014, 41(6): 1133-1144. [杨冲, 张扬勇, 方智远, 等. 甘蓝叶色黄化突变体YL-1的光合生理特性及其叶绿体的超微结构 [J]. 园艺学报, 2014, 41(6): 1133-1144. doi: 10.16420/j.issn.0513-353x.2014.06.028.]
    [7] LIU H Y, ZHOU F, LI J, et al. Anatomical structure and photosynthetic characteristics of a yellow leaf mutant YL1 in sesame (Sesamum indicum L.) [J]. Acta Agron Sin, 2017, 43(12): 1856-1863. [刘红艳, 周芳, 李俊, 等. 芝麻黄化突变体YL1的叶片解剖学及光合特性 [J]. 作物学报, 2017, 43(12): 1856-1863. doi: 10.3724/SP.J.1006.2017.01856.]
    [8] JIN K, HUANG J A, XIONG L G, et al. Research of theanine-related genes expressed in etiolated tea plant Camellia sinensis) [J]. J Tea Sci, 2021, 41(1): 40-47. [金可, 黄建安, 熊立瑰, 等. 黄化变异茶树石门黄叶理化分析及茶氨酸相关基因表达研究 [J]. 茶叶科学, 2021, 41 (1): 40-47. doi: 10.3969/j.issn.1000-369X.2021.01.006.]
    [9] GUO J Y, LIU B M, YANG H J, et al. Genetic analysis and gene mapping of the yellow midrib leaf mutant (yml) in rice (Oryza sativa L.) [J]. Acta Agron Sin, 2022, 48(12): 3120-3129. [郭均瑶, 刘斌美, 杨惠杰, 等. 水稻叶脉黄化突变体yml的遗传分析及基因定位 [J]. 作物学报, 2022, 48(12): 3120-3129. doi: 10.3724/SP.J.1006.2022.12077.]
    [10] LU Z H, MO X J, JIANG Y L, et al. The preliminary study of chlorina mutant in Dendranthema morifolium [J]. J SW For Univ, 2017, 37(2): 60-68. [卢珍红, 莫锡君, 蒋亚莲, 等. 菊花黄化突变体初步研究 [J]. 西南林业大学学报, 2017, 37(2): 60-68. doi: 10.11929/j.issn. 2095-1914.2017.02.010.]
    [11] MA Q P. Albino mechanism of tea plant based on multi-omics analysis [D]. Nanjing: Nanjing Agricultural University, 2019. [马青平. 基于多组学分析的茶树白化机制研究 [D]. 南京: 南京农业大学, 2019.]
    [12] WANG C X, TIAN W W, TIAN M, et al. The preliminary study of xantha mutants in Oncidium [J]. J Nucl Agric Sci, 2013, 27(12): 1845-1852. [王彩霞, 田韦韦, 田敏, 等. 文心兰黄化突变体的初步研究 [J]. 核农学报, 2013, 27(12): 1845-1852.]
    [13] YI L, HU X Y, WEI X, et al. Effects of shade on the leaf microstructure and chloroplast ultrastructure of the invasive Wedelia trilobata, the native W. chinensis and their hybrid [J]. Guihaia, 2014, 34(1): 19-26. [易立, 胡晓颖, 韦霄, 等. 遮荫对南美蟛蜞菊、蟛蜞菊及其自然杂交种叶片显微结构及叶绿体超微结构的影响 [J]. 广西植物, 2014, 34(1): 19-26. doi: 10.3969/j.issn.1000-3142.2014.01.005.]
    [14] LIU X. Experimental Guidance of Plant Physiology [M]. Beijing: China Agriculture Press, 2015: 25-27. [刘新. 植物生理学实验指导 [M]. 北京: 中国农业出版社, 2015: 25-27.]
    [15] FALBEL T G, MEEHL J B, STAEHELIN L A. Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis [J]. Plant Physiol, 1996, 112(2): 821-832. doi: 10.1104/pp.112.2.821.
    [16] MURRAY D L, KOHORN B D. Chloroplasts of Arabidopsis thaliana homozygous for the ch-1 locus lack chlorophyll b, lack stable LHCPII and have stacked thylakoids [J]. Plant Mol Biol, 1991, 16(1): 71-79. doi: 10.1007/BF00017918.
    [17] BELLEMARE G, BARTLETT S G, CHUA N H. Biosynthesis of chlorophyll a/b-binding polypeptides in wild type and the chlorina f2 mutant of barley [J]. J Biol Chem, 1982, 257(13): 7762-7767. doi: 10. 1016/S0021-925818)34446-6.
    [18] HIRAYAMA T, SHINOZAKI K. Perception and transduction of abscisic acid signals: Keys to the function of the versatile plant hormone ABA [J]. Trends Plant Sci, 2007, 12(8): 343-351. doi: 10.1016/j.tplants.2007.06. 013.
    [19] XIAO H G, YANG H W, RAO Y, et al. Photosynthetic characteristics and chlorophyll fluorescence kinetic parameters analyses of chlorophyll-reduced mutant in Brassica napus L. [J]. Acta Agron Sin, 2013, 39(3): 520-529. [肖华贵, 杨焕文, 饶勇, 等. 甘蓝型油菜黄化突变体的光合特性及叶绿素荧光参数分析 [J]. 作物学报, 2013, 39(3): 520-529. doi: 10.3724/SP.J.1006.2013.00520.]
    [20] HUANG X J, XU Z H, NIU R M, et al. Effect of etiolation on photosynthesis and chlorophyll fluorescence of ‘Cabernet Sauvignon’ grapes [J]. Nonwood For Res, 2020, 38(3): 190-199. [黄小晶, 许泽华, 牛锐敏, 等. 叶片黄化对‘赤霞珠’葡萄光合及叶绿素荧光特性的影响 [J]. 经济林研究, 2020, 38(3): 190-199. doi: 10.14067/j.cnki.1003-8981. 2020.03.022.]
    [21] WANG Y F, JIANG Z D, ZHANG A, et al. Changes of photosynthetic pigment content and chlorophyll fluorescence parameters during leaf etiolation of Ginkgo biloba L. [J]. SW China J Agric Sci, 2020, 33(10): 2220-2224. [王艺斐, 姜泽东, 张敖, 等. 银杏叶片黄化过程中光合色素含量和叶绿素荧光参数的变化特征研究 [J]. 西南农业学报, 2020, 33(10): 2220-2224. doi: 10.16213/j.cnki.scjas.2020.10.013.]
    [22] LIN X Y, WANG P J, YANG R X, et al. The albino mechanism of a new high theanine tea cultivar Fuhuang 1 [J]. Sci Agric Sin, 2022, 55 (9): 1831-1845. [林馨颖, 王鹏杰, 杨如兴, 等. 高茶氨酸茶树新品系‘福黄1号’黄化变异机理 [J]. 中国农业科学, 2022, 55(9): 1831-1845. doi: 10.3864/j.issn.0578-1752.2022.09.012.]
    [23] LIU X Q, LUO J. Advances of technologies and research in plant metabolomics [J]. Sci Technol Rev, 2015, 33(16): 33-38. [刘贤青, 罗杰. 植物代谢组学技术研究进展 [J]. 科技导报, 2015, 33(16): 33-38. doi: 10.3981/j.issn.1000-7857.2015.16.004.]
    [24] QI X Q, WANG Y L, CHEN X Y. Plant Metabolomics: Methods and Applications [M]. Beijing: Chemical Industry Press, 2011. [漆小泉, 王玉兰, 陈晓亚. 植物代谢组学——方法与应用 [M]. 北京: 化学工业出版社, 2011.]
    [25] YAMAZAKI M, NAKAJIMA J I, YAMANASHI M, et al. Metabolomics and differential gene expression in anthocyanin chemovarietal forms of Perilla frutescens [J]. Phytochemistry, 2003, 62(6): 987-995. doi: 10. 1016/S0031-942202)00721-5.
    [26] FRANKE R, SCHREIBER L. Suberin: A biopolyester forming apoplastic plant interfaces [J]. Curr Opin Plant Biol, 2007, 10(3): 252-259.
    [27] SCHREIBER L. Transport barriers made of cutin, suberin and associated waxes [J]. Trends Plant Sci, 2010, 15(10): 546-553. doi: 10.1016/j. tplants.2010.06.004.
    [28] LULAI E C, SUTTLE J C, PEDERSON S M. Regulatory involvement of abscisic acid in potato tuber wound-healing [J]. J Exp Bot, 2008, 59 (6): 1175-1186. doi: 10.1093/jxb/ern019.
    [29] KILIAN J, WHITEHEAD D, HORAK J, et al. The AtGenExpress global stress expression data set: Protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses [J]. Plant J, 2007, 50(2): 347-363. doi: 10.1111/j.1365-313X.2007.03052.x.
    [30] OBAYASHI T, HAYASHI S, SAEKI M, et al. ATTED-II provides coexpressed gene networks for Arabidopsis [J]. Nucl Acids Res, 2009, 37(S1): D987-D991. doi: 10.1093/nar/gkn807.
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

陈汉鑫,林艺辉,马馨怡,林秀芳.珍珠芦荟失绿突变体‘MT-1’的农艺性状、解剖结构、光合特性和代谢组特征分析[J].热带亚热带植物学报,2024,32(3):417~426

Copy
Share
Article Metrics
  • Abstract:89
  • PDF: 3374
  • HTML: 3220
  • Cited by: 0
History
  • Received:June 06,2023
  • Revised:August 16,2023
  • Online: June 04,2024
Article QR Code
You are the first4641256 Visitors
Governing Body：中国科学院
Organizer：中国科学院华南植物园 广东省植物学会
Address：Xingke Road 723,Tianhe District,Guangzhou,China
Phone：+86 (0)20-3725 2514
Journal of Tropical and Subtropical Botany ® 2025 All Rights Reserved