IQM3 Regulated Main Root Length of Arabidopsis thaliana at Downstream of CO under Long Sunshine
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    Abstract:

    In Arabidopsis thaliana, IQM family is a calmodulin-binding protein family containing IQ motifs, IQM3 mutation can increase main root length, CO (CONSTANS) is an important member of photoperiodic floral regulation pathway, CO mutation can shorten taproot length. The genetic relationship between IQM3 and CO gene was studied by constructing double mutants of IQM3 and CO. The results showed that the first exon in co-12 sequence of the new CO mutant was missing 9 base ACTTGCTAG, which contains the restriction endonucrenase BfaI restriction site CTAG. A molecular marker was established to identify the mutant. When the cross-coding PCR product was cleaved by BfaI enzyme, four fragments were obtained from wild-type Col because it had three sites, three fragments were obtained from co-12 because it had only two sites, and five fragments were obtained from CO/co-12 heterozygote. Under long sunshine, the main root length of co-12 was shorter than that of wild type Col, and that of iqm3-2 was longer than that of wild type, while the phenotype of double mutant co-12 iqm3-2 was more favorable to iqm3-2. Therefore, IQM3 acts downstream of CO to regulate main root length under long day in Arabidopsis.

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    [1] KELLY J W G, LANDHÄUSSER S M, CHOW P S. The impact of light quality and quantity on root-to-shoot ratio and root carbon reserves in aspen seedling stock [J]. New For, 2015, 46(4): 527-545. doi: 10.1007/s11056-015-9473-9.
    [2] LI L, LI X, LIU Y W, et al. Flowering responses to light and temperature [J]. Sci China Life Sci, 2016, 46(3): 253-259. [李莉, 李旭, 刘亚文, 等. 光和温度调控开花时间的研究进展[J]. 中国科学: 生命科学, 2016, 46(3): 253-259. doi: 10.1360/05SCLS-2015-0122. R2.]
    [3] FILLEUR S, WALCH-LIU P, GAN Y, et al. Nitrate and glutamate sensing by plant roots [J]. Biochem Soc Trans, 2005, 33(1): 283-286. doi: 10.1042/BST0330283.
    [4] KUMARI S, YADAV S, PATRA D, et al. Uncovering the molecular signature underlying the light intensity-dependent root development in Arabidopsis thaliana [J]. BMC Genom, 2019, 20(1): 596. doi: 10.1186/ s12864-019-5933-5.
    [5] SHIM J S, KUBOTA A, IMAIZUMI T. Circadian clock and photo-periodic flowering in Arabidopsis: CONSTANS is a hub for signal integration [J]. Plant Physiol, 2017, 173(1): 5-15. doi: 10.1104/pp. 16.01327.
    [6] PUTTERILL J, ROBSON F, LEE K, et al. The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors [J]. Cell, 1995, 80(6): 847- 857. doi: 10.1016/0092-8674(95)90288-0.
    [7] ROBSON F C, COSTA M M, HEPWORTH S R, et al. Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants [J]. Plant J, 2001, 28(6): 619-631. doi: 10.1046/j.1365-313x. 2001.01163.x.
    [8] KOORNNEEF M, HANHART C J, VAN DER VEEN J H. A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana [J]. Mol Gen Genet, 1991, 229(1): 57-66. doi: 10.1007/BF00264213.
    [9] YOO S K, WU X L, LEE J S, et al. AGAMOUS-LIKE 6 is a floral promoter that negatively regulates the FLC/MAF clade genes and positively regulates FT in Arabidopsis [J]. Plant J, 2011, 65(1): 62-76. doi: 10.1111/J.1365-313X.2010.04402.X.
    [10] NING Y Q, CHEN Q, LIN R N, et al. The HDA19 histone deacetylase complex is involved in the regulation of flowering time in a photoperiod-dependent manner [J]. Plant J, 2019, 98(3): 448-464. doi: 10.1111/tpj. 14229.
    [11] FENG Y J, LONG H. Vegetative phase change delayed in late-flowering mutants co-2and ft-1 in Arabidopsis thaliana [J]. Chin J Biol, 2013, 30(4): 46-49. [冯延娇, 龙鸿. 拟南芥晚花突变co-2ft-1延迟营养生长时相转变[J]. 生物学杂志, 2013, 30(4): 46-49. doi: 10.3969/ j.issn.2095-1736.2013.04.046.]
    [12] MAYFIELD J D, PAUL A L, FERL R J. The 14-3-3 proteins of Arabidopsis regulate root growth and chloroplast development as components of the photosensory system [J]. J Exp Bot, 2012, 63(8): 3061-3070. doi: 10.1093/jxb/ers022.
    [13] DEFALCO T A, BENDER K W, SNEDDEN W A. Breaking the code: Ca2+ sensors in plant signalling [J]. Biochem J, 2010, 425(1): 27-40. doi: 10.1042/BJ20091147.
    [14] YANG T B, POOVAIAH B W. Calcium/calmodulin-mediated signal network in plants [J]. Trends Plant Sci, 2003, 8(10): 505-512. doi: 10.1016/j.tplants.2003.09.004.
    [15] TIAN C E, ZHOU Y P. Research progress in plant IQ motif-containing calmodulin-binding proteins [J]. Chin Bull Bot, 2013, 48(4): 447-460. [田长恩, 周玉萍. 植物具IQ基序的钙调素结合蛋白的研究进展[J]. 植物学报, 2013, 48(4): 447-460. doi: 10.3724/SP.J.1259.2013.00447.]
    [16] CARAFOLI E, KREBS J. Why Calcium? How calcium became the best communicator [J]. J Biol Chem, 2016, 291(40): 20849-20857. doi: 10.1074/jbc.R116.735894.
    [17] TSAI Y C, DELK N A, CHOWDHURY N I, et al. Arabidopsis potential calcium sensors regulate nitric oxide levels and the transition to flowering [J]. Plant Sign Behav, 2007, 2(6): 446-454. doi: 10.4161/ psb.2.6.4695.
    [18] ZHOU Y P, CHEN Y Z, YAMAMOTO K T, et al. Sequence and expression analysis of the Arabidopsis IQM family [J]. Acta Physiol Plant, 2010, 32(1): 191-198. doi: 10.1007/s11738-009-0398-9.
    [19] MO Z Z, HUANG Z K, ZHOU Y P, et al. IQM1 is involved in response of primary root growth to methyl jasmonate in Arabidopsis [J]. Lett Biotechnol, 2015, 26(3): 399-402. [莫忠蓁, 黄章科, 周玉萍, 等. 拟南芥IQM1涉及主根生长对茉莉酸甲酯的反应[J]. 生物技术通讯, 2015, 26(3): 399-402. doi: 10.3969/j.issn.1009-0002.2015.03.022.]
    [20] XU H, FENG Y J, FAN T, et al. Disruption of IQM3 reduces the number of lateral roots and increases the length of primary root in Arabidopsis seedlings [J]. Plant Physiol J, 2019, 55(5): 629-634. [徐浩, 冯奕嘉, 范甜, 等. 拟南芥IQM3基因突变减少幼苗的侧根数量和增加主根长度[J]. 植物生理学报, 2019, 55(5): 629-634. doi: 10. 13592/j.cnki.ppj.2019.1001.]
    [21] JIANG B, ZHANG Y L, ZHOU D Y. Common Experiment Methods of Molecular Biology [M]. Beijing: People’s Military Medical Press, 1996. [姜泊, 张亚历, 周殿元. 分子生物学常用实验方法[M]. 北京: 人民军医出版社, 1996.]
    [22] ZHOU Y P, ZHAO J, HE T, et al. Expression analysis of Arabidopsis thaliana IQM3 gene and identification of iqm3 mutants [J]. J Trop Subtrop Bot, 2009, 17(4): 365-370. [周玉萍, 赵军, 何滔, 等. 拟南芥IQM3基因的表达分析及其突变体的鉴定[J]. 热带亚热带植物学报, 2009, 17(4): 365-370. doi: 10.3969/j.issn.1005-3395.2009.04.009.]
    [23] KHANNA R, KRONMILLER B, MASZLE D R, et al. The Arabidopsis B-box zinc finger family [J]. Plant Cell, 2009, 21(11): 3416- 3420. doi: 10.1105/tpc.109.069088.
    [24] DODD A N, LOVE J, WEBB A A R. The plant clock shows its metal: Circadian regulation of cytosolic free Ca2+ [J]. Trends Plant Sci, 2005, 10(1): 15-21. doi: 10.1016/j.tplants.2004.12.001.
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余洁雨,李树强,王韫慧,范甜,吕天晓,周玉萍,田长恩.长日照下IQM3CO的下游调控拟南芥主根长度[J].热带亚热带植物学报,2024,32(4):451~457

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  • Received:April 01,2023
  • Revised:May 08,2023
  • Online: August 21,2024
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