Abstract:In order to study the chemical constituents in butanol fraction of ethanol extract of Dendrobium nobile, column chromatographic techniques including silica gel, Sephadex LH-20, revised silica gel and HPLC were used to separate the compounds, and spectroscopic techniques including NMR and MS were implied to determine the structures. Totally 3 compounds were isolated, and their structures were identified as 2,11-epoxy-11,13-dihydro-xypicrotoxano-3(15)-lactone (1), 2,4,6-trimethoxyphenol-1-O-β-d-glycoside (2), 3-methylbutan-1-ol β-d-gluco-pyranoside (3). Compound 1 was a new picrotoxane-type sesquiterpene, which is one of the representative components in D. nobile. However, none of the compounds showedα-glucosidase and tyrosinase inhibitory activity, none of them showed activity, and compound 1 show no neuroprotective activity either.