Abstract:Hevea brasiliensis seeds generally cultivated in sand bed. Sand is a non-renewable resource. In order to choose a suitable seed cultivation method to replace the dependence on sand, the growth vigor, physiological indexes and nutrient contents of H. brasiliensis seedlings were compared, when the first leaf was stable through hydroponic, airborne and sand cultures. The results showed that the above-ground height, stem diameter and leaf area of hydroponic seedlings had the best growth, and the strong seedling index and biomass were the highest, but the roots were too long and relatively thin. The contents of soluble sugar, malondialdehyde, free proline and superoxide dismutase activity were low in the leaves, stems and roots of hydroponic culture. There was no significant difference in the contents of chlorophyll, carotenoid in leaf and stem and root activity between hydroponic culture and airborne culture, which were all higher than sand culture. The contents of nitrogen and phosphorus in leaf, stem and root of hydroponic culture were the lowest, while those of sand culture were the highest. The content of potassium in roots and stems of hydroponic seedlings was higher, and the content of potassium in leaves was not significantly different from that in airborne culture and sand cultivation. The content of potassium in leaves, stems and roots of airborne culture was the lowest. Hydroponic culture can promote the growth of seedlings, reduce drought stress and improve nutrient utilization, but it is necessary to regulate the root system and build a good root mass. The seedling growth in airborne culture is weak, so the method needs to be further perfected.