Studies on the Causes of Seed Dormancy of Phoebe sheareri and Rapid Removal Methods
Author:
  • Article
  • | |
  • Metrics
  • |
  • Reference [38]
  • |
  • Related [20]
  • | | |
  • Comments
    Abstract:

    By understanding the causes of seed dormancy of Phoebe sheareri, mechanical, acid-alkali corrosion and lamination treatment (5 ℃-25 ℃) were used to study the best methods to relieve seed dormancy. The results showed that the low permeability of seed coat was the main reason for inhibiting seed germination, and the seed dormancy type was physical dormancy. Acid and alkali corrosion treatments could not effectively break seed dormancy. After the seeds were treated with concentrated sulfuric acid and sodium hydroxide for different time (1-25 min), the germination rate was increased by 33%-55%, but the seeds were seriously damaged, and the decay rate was as high as 30%-97%. Both mechanical treatment and stratification treatments could effectively break seed dormancy, and removing whole seed coat by mechanical treatment had the best effect, and seed germination rate and germination potential increased by 99.33% and 76.00%, respectively. But this method was time-consuming and laborious. The seeds under 25 ℃ warm stratification for 45 d, 25 ℃/15 ℃ and 5 ℃/25 ℃/5 ℃ variable temperature stratification for 60 d could effectively promote germination, with the germination rate and germination potential increased by 89%-93% and 79%-83%, respectively, and there were no obvious differences in germination rate and germination potential among these types of stratification. However, compared with warm stratification, variable temperature stratification requires longer stratification time and higher seed decay rate. Therefore, 25 ℃ warm stratification for 45 d was the optimal way for breaking seed dormancy of P. sheareri.

    Reference
    [1] LI X, LI X J, SHI F H, et al. Effects of slow-release fertilizer amount on the growth of container seedlings of Phoebe sheareri [J]. J NW For Univ, 2022, 37(4): 152-157. [李鑫, 李晓菁, 史锋厚, 等. 缓释肥施用量对紫楠容器苗生长的影响 [J]. 西北林学院学报, 2022, 37(4): 152-157. doi: 10.3969/j.issn.1001-7461.2022.04.20.]
    [2] LIU D R, ZHU Y, WANG J H, et al. Community structure and its species diversity of Phoebe sheareri in Liyang of Jingsu Province [J]. J Chin Urban For, 2021, 19(4): 12-16. [刘冬瑞, 朱颖, 王金虎, 等. 江苏溧阳紫楠群落结构及物种多样性特征 [J]. 中国城市林业, 2021, 19(4): 12-16. doi: 10.12169/zgcsly.2019.12.18.0001.]
    [3] WANG Y, MA X H, LU Y F, et al. Assessing the current genetic structure of 21 remnant populations and predicting the impacts of climate change on the geographic distribution of Phoebe sheareri in southern China [J]. Sci Total Environ, 2022, 846: 157391. doi: 10. 1016/j.scitotenv.2022.157391.
    [4] LI J, LU Y F, YANG A N, et al. Species diversity of natural Phoebe sheareri communities with different disturbance intensities [J]. J Zhejiang A&F Univ, 2019, 36(2): 279-288. [李军, 陆云峰, 杨安娜, 等. 紫楠天然群落物种多样性对不同干扰强度的响应 [J]. 浙江农林大学学报, 2019, 36(2): 279-288. doi: 10.11833/j.issn.2095-0756. 2019.02.009.]
    [5] SHI H W, DENG Y C, LIU S C, et al. Technology of container seedling cultivation of Phoebe sheareri in Wuhan [J]. Hubei For Sci Technol, 2020, 49(5): 83-84. [史红文, 邓永成, 刘淑超, 等. 武汉地区紫楠容器育苗技术 [J]. 湖北林业科技, 2020, 49(5): 83-84. doi: 10.3969/j. issn.1004-3020.2020.05.027.]
    [6] XU H D, ZHU B, WEI X M, et al. Root functional traits mediate rhizosphere soil carbon stability in a subtropical forest [J]. Soil Boil Biochem, 2021, 162: 108431. doi: 10.1016/j.soilbio.2021.108431.
    [7] HUANG X, WU Y, LI Y, et al. First report of Colletotrichum fructicola causing anthracnose on Phoebe sheareri in China [J]. Plant Dis, 2022, 106(7): 1994. doi: 10.1094/PDIS-08-21-1841-PDN.
    [8] LIU H, LIU M, TANG W, et al. Wood structural characteristics of Phoebe sheareri [J]. J NE For Univ, 2017, 45(2): 53-56. [刘虎, 刘敏, 汤雯, 等. 紫楠木材的构造特征 [J]. 东北林业大学学报, 2017, 45 (2): 53-56. doi: 10.13759/j.cnki.dlxb.2017.02.011.]
    [9] CHEN A L, ZHAO Z H, GONG W, et al. Predicting suitable distribution areas of Phoebe sheareri in China under climate change [J]. J Trop Subtrop Bot, 2020, 28(5): 435-444. [陈爱莉, 赵志华, 龚伟, 等. 气候变化背景下紫楠在中国的适宜分布区模拟 [J]. 热带亚热带植物学报, 2020, 28(5): 435-444. doi: 10.11926/jtsb.4201.]
    [10] FAN Y, LOU Y K, KU W P, et al. Age structure and spatial point pattern of Phoebe sheareri population in Mount Tianmu [J]. J Zhejiang A & F Univ, 2020, 37(6): 1027-1035. [范忆, 楼一恺, 库伟鹏, 等. 天目山紫楠种群年龄结构与点格局分析 [J]. 浙江农林大学学报, 2020, 37(6): 1027-1035. doi: 10.11833/j.issn.2095-0756.20190631.]
    [11] MENG Z L, LIANG R L. Analysis of the essential oils obtained from the fresh leaves of Phoebe sheareri and Phoebe bournei in Guangxi and Phoebe zhennan in Emei Area of Sichuan Province [J]. Flavour Frag Cosmet, 2019(2): 1-8. [孟中磊, 梁瑞龙. 广西紫楠、闽楠与四川峨眉桢楠鲜叶精油成分分析 [J]. 香料香精化妆品, 2019(2): 1-8. doi: 10.3969/j.issn.1000-4475.2019.02.001.]
    [12] JIANG H H. Study on isolation and identification of Phoebe sheareri and its bioactivities [D]. Shanghai: Shanghai Ocean University, 2017. [姜浩瀚. 紫楠有效成分分离纯化鉴定及其生物活性研究 [D]. 上海: 上海海洋大学, 2017.]
    [13] SHI X H, TIAN L J, SHI Z L. Study on seed dormancy physiology of Phoebe sheareri [J]. Seed, 1988(1): 32-33. [史晓华, 田丽洁, 史忠礼. 紫楠种子休眠生理的研究 [J]. 种子, 1988(1): 32-33. doi: 10.16590/ j.cnki.1001-4705.1988.01.045.]
    [14] LI Z, WANG S J, LIU C L, et al. Study on seed germination characteristics of Phoebe sheareri and Phoebe chekiangensis [J]. N Hort, 2012(7): 58-60. [李珍, 王素娟, 刘纯玲, 等. 紫楠及浙江楠种子萌发特性研究 [J]. 北方园艺, 2012(7): 58-60.]
    [15] CHEN Q H, SHAO A H, JIANG X, et al. Effects of different pretreatment on the seed germination of Phoebe sheareri [J]. Contern Hort, 2018(7): 37-39. [陈勤华, 邵爱华, 蒋旭, 等. 不同预处理对紫楠种子萌发的影响 [J]. 现代园艺, 2018(7): 37-39. doi: 10.14051/j.cnki. xdyy.2018.07.016.]
    [16] JIANG Z Q, LI C Z, ZHOU X, et al. Study on dormancy characteristics and removal measures of Phoebe sheareri seeds [J]. Seed, 2020, 39(12): 92-94. [姜宗庆, 李成忠, 周霞, 等. 紫楠种子休眠特性及解除措施研究 [J]. 种子, 2020, 39(12): 92-94. doi: 10.16590/j.cnki.1001-4705. 2020.12.092.]
    [17] The State Bureau of Quality and Technical Supervision. GB/T 2772—1999 Rules for forest tree seed testing [S]. Beijing: Standards Press of China, 1999: 19-20. [国家质量监督局. GB/T 2772—1999 林木种子检验规程 [S]. 北京: 中国标准出版社, 1999: 19-20.]
    [18] LI Y, LI H Y, PANG S F, et al. Research on the physiological mechanism of GA and ABA regulating Ginseng Radix et Rhizoma seed dormancy [J]. Mol Plant Breed, 2023, 21(12): 4036-4042. [李渊, 李红钰, 逄世峰, 等. GA和ABA调控人参种子休眠的生理机制 [J]. 分子植物育种, 2023, 21(12): 4036-4042. doi: 10.13271/j.mpb.021.004 036.]
    [19] YE Q H, CENG D, CHEN Z D, et al. Plant Biology [M]. Xiamen: Xiamen University Press, 2002: 47-49. [叶庆华, 曾定, 陈振端, 等. 植物生物学 [M]. 厦门: 厦门大学出版社, 2002: 47-49.]
    [20] ZHOU B X. Seeds of Woody Plants in China [M]. Beijing: China Forestry Publishing House, 2001: 767-770. [国家林业局国有林场, 林木种苗工作总站. 中国木本植物种子 [M]. 北京: 中国林业出版社, 2001: 767-770.]
    [21] LI Y, SHEN Y B. A study on the seed dormancy mechanism and ways of dormancy breaking in Hovenia acerba Lindl. [J]. J Nanjing For Univ (Nat Sci), 2014, 38(2): 57-62. [李英, 沈永宝. 枳椇种子休眠原因及解除方法 [J]. 南京林业大学学报(自然科学版), 2014, 38(2): 57-62. doi: 10.3969/j.issn.1000-2006.2014.02.012.]
    [22] LIN X X, CHEN Z D, LIN Q J, et al. Study on the germination characters of Phyllagathis fordii [J]. China Agri Sci Bull, 2013, 29(28): 156-159. [林秀香, 陈振东, 林秋金, 等. 叶底红种子发芽特性研究 [J]. 中国农学通报, 2013, 29(28): 156-159. doi: 10.3969/j.issn.10006850.2013.28.030.]
    [23] BASKIN J M, BASKIN C C. A classification system for seed dormancy [J]. Seed Sci Res, 2004, 14(1): 1-16. doi: 10.1079/SSR2003150.
    [24] CAO S S. Study on seed dormancy characteristics of Sorbus amabilis Cheng ex Yü [D]. Nanjing: Nanjing Forestry University, 2009. [曹珊珊. 黄山花楸种子休眠特性研究 [D]. 南京: 南京林业大学, 2009.]
    [25] SONG W G. Causes of dormancy and germination method of seeds for four kinds of Acer (Tourn.) Linn. [J]. Protect For Sci Tech, 2016(8): 31-33. [宋伟国. 4种槭树种子的休眠原因及催芽方法试验 [J]. 防护林科技, 2016(8): 31-33. doi: 10.13601/j.issn.1005-5215.2016.08. 011.]
    [26] STEINBAUER G P. Dormancy and germination of Fraxinus seeds [J]. Plant Physiol, 1937, 12(3): 813-824. doi: 10.1104/pp.12.3.813.
    [27] BETHKE P C, GUBLER F, JACOBSEN J V, et al. Dormancy of Arabidopsis seeds and barley grains can be broken by nitric oxide [J]. Planta, 2004, 219(5): 847-855. doi: 10.1007/s00425-004-1282-x.
    [28] MEYER S E, PENDLETON R L. Genetic regulation of seed dormancy in Purshia tridentata (Rosaceae) [J]. Ann Bot, 2000, 85(4): 521-529. doi: 10.1006/anbo.1999.109.
    [29] ZHANG P. Study on seed dormancy mechanism and seedling cultivation of Oplopanax elatus [D]. Harbin: Northeast Forestry University, 2004. [张鹏. 东北刺人参种子休眠机制及播种育苗技术研究 [D]. 哈尔滨: 东北林业大学, 2004.]
    [30] KIM J H, LEE A K, SUH J K. Effect of warm and cold stratification, and ethanol treatment on germination of Corylopsis seeds [J]. Hort Sci, 2016, 43(2): 84-91. doi: 10.17221/351/2014-HORTSCI.
    [31] QIAN X Y. Physiological mechanism of breaking seed dormancy and effect of seedling quality on Paeonia ostii by different tempreture and gibberellic acid [D]. Nanjing: Nanjing Agricultural University, 2009. [钱仙云. 温度与GA3解除凤丹种子休眠的生理代谢变化及对其幼苗质量的影响 [D]. 南京: 南京农业大学, 2009.]
    [32] LI J C, ZHUANG X D. Effects of stored substances on Taxus cuspidate seeds through different tempreture and and GA3 treatment [J]. Agric Jilin, 2015(4): 88-89. [李晶莼, 庄晓冬. 温度及GA3处理对东北红豆杉种子贮藏物质的影响 [J]. 吉林农业, 2015(4): 88-89. doi: 10. 14025/j.cnki.jlny.2015.04.036.]
    [33] DENG H J, LIU H L, NIU Y, et al. Effects of stratification treatments on dormancy in Amorphophallus albus seeds [J]. J SW Univ (Nat Sci), 2018, 40(7): 38-43. [邓慧君, 刘海利, 牛义, 等. 层积处理对打破白魔芋实生种子休眠的影响 [J]. 西南大学学报(自然科学版), 2018, 40(7): 38-43. doi: 10.13718/j.cnki.xdzk.2018.07.006.]
    [34] KONDO T, SATO C, BASKIN J M, et al. Post-dispersal embryo development, germination phenology, and seed dormancy in Cardiocrinum cordatum var. glehnii (Liliaceae s. str.), a perennial herb of the broadleaved deciduous forest in Japan [J]. Am J Bot, 2006, 93(6): 849-859.
    [35] FU Q, YANG Q H, YE W H. Summarization on methods to relieve seed dormancy [J]. J Guangxi Agric Biol Sci, 2003, 22(3): 230-234. [傅强, 杨期和, 叶万辉. 种子休眠的解除方法 [J]. 广西农业生物科学, 2003, 22(3): 230-234.]
    [36] VAN ASSCHE J A, VANLERBERGHE K A. The role of temperature on the dormancy cycle of seeds of Rumex obtusifolius L. [J]. Funct Ecol, 1989, 3(1): 107-115. doi: 10.2307/2389681.
    [37] THOMPSON P A. Germination of Lycopus europaeus L. in response to fluctuating temperatures and light [J]. J Exp Bot, 1969, 20(1): 1-11. doi: 10.1093/jxb/20.1.1.
    [38] BASKIN C C, ZACKRISSON O, BASKIN J M. Role of warm stratification in promoting germination of seeds of Empetrum hermaphroditum (Empetraceae), a circumboreal species with a stony endocarp [J]. Am J Bot, 2002, 89(3): 486-493. doi: 10.3732/ajb.89.3.486.
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

林秀灵,史锋厚,余中华,龙字文,蒋学莉,沈永宝.紫楠种子休眠原因和快速解除方法研究[J].热带亚热带植物学报,2024,32(3):387~396

Copy
Share
Article Metrics
  • Abstract:160
  • PDF: 3332
  • HTML: 3223
  • Cited by: 0
History
  • Received:November 26,2022
  • Revised:August 21,2023
  • Online: June 04,2024
Article QR Code