Cen Xiangni
School of Chemical Engineering and Technology, Hainan University, Haikou 570228, ChinaFan Yiying
School of Chemical Engineering and Technology, Hainan University, Haikou 570228, ChinaLiu Haowen
School of Chemical Engineering and Technology, Hainan University, Haikou 570228, ChinaLi Jiayin
School of Chemical Engineering and Technology, Hainan University, Haikou 570228, ChinaWang Chuanlin
School of Chemical Engineering and Technology, Hainan University, Haikou 570228, ChinaLiu Pinghuai
School of Chemical Engineering and Technology, Hainan University, Haikou 570228, ChinaTo understand the chemical constituents and antibacterial activities of Trigonostemon heterophyllus, six compounds, including five diterpenoids and one lignan, were isolated and purified by silica gel column chromate- graphy, gel column chromatography and high performance liquid chromatography. Based on spectroscopic data, their structures were identified as trigonochinene E (1), neoboutomannin (2), 6,9-O-dedimethyl trigonostemone (3), stelltian B (4), 3,4-secosonderianol (5), biondinin A (6). The inhibitory activity and MIC values of compounds against a Gram-negative bacterium, Escherichia coli, and a Gram-positive bacterium, Pneumococcus, were detected by Oxford Cup method and double dilution method. Compounds 1, 2, 3 and 5 had inhibitory effects on Escherichia coli with MIC values of 9.375, 18.75, 18.75 and 18.75 μg/mL, respectively. Compounds 2, 3, 4, and 6 were isolated from T. heterophyllus for the first time, and compound 4 was isolated from Trigonostemon genus for the first time. Except compound 4, all the other compounds had certain antibacterial activity, and compounds 1, 2 and 3 had stronger inhibitory effect on Escherichia coli than Kanamycin sulfate positive control.
岑祥妮,范艺灜,刘浩文,李佳莹,王川林,刘平怀.异叶三宝木的二萜成分及抗菌活性研究[J].热带亚热带植物学报,2023,31(4):602~606
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