Studies on Metabolites of Endophytic Fungus Aspergillus fumigatus Isolated from Paris polyphylla var. yunnanensis

Abstract:

To study metabolites of endophytic fungus Aspergillus fumigatus isolated from Paris polyphylla var. yunnanensis, thirty-one compounds were obtained from fermentation broth and mycelium by several chromato- graphic techniques, such as silica gel column chromatography, sephadex LH-20 and semi-preparative HPLC. Based on physicochemical properties and spectral data, their structures were identified as tryprostatin B (1), tryprostatin A (2), 12,13-dihydroxyfumitremorgin C (3), cyclotryprostatin B (4), 14-norpseurotin A (5), pseu- rotine F1 (6), pseurotine F2 (7), azaspirofuran A (8), pseurotin D (9), spirotryprostatin K (10), 6-methoxy- spirotryprostatin B (11), 7-deacetylpyripyropene A (12), fumagillin (13), fumagillene A (14), 5,9-dihydroxy-β- trans-bergamotene (15), 4-hydroxyphenylacetic acid (16), demethoxyfumitremorgin C (17), fumiquinazoline J (18), helvolic acid (19), ergosterol (20), ergosterol endoperoxide (21), 4,4-dimethyl-5α-ergosta-8,24(28)-dien- 3β-ol (22), lanosterol (23), linoleic acid (24), oleic acid (25), fumitremorgin C (26), fumitremorgin B (27), verruculogen (28), pseurotin A (29), fumiquinazoline C (30), and questin (31). Compounds 1-16 and 17-25 were isolated from fermentation broth and mycelium, respectively, while compounds 26-31 were isolated from both fermentation broth and mycelium. Compounds 15 and 23 were isolated from A. fumigatus for the first time. In addition, the compounds 26-29 were evaluated for their acetylcholinesterase inhibition activities and tobacco black shank inhibition activities, compounds 26 and 27 showed slight activities against pathogenic fungi of tobacco black shank with inhibition rate of 19.64% and 17.86%, respectively.

Key words:Paris polyphylla var. yunnanensis;Endophytic fungus;Metabolite;Tobacco black shank inhibition activity