Abstract:In order to explore the genes related to pine resin biosynthesis in Pinus elliottii, a total of 12 samples of xylem at different resin collection stages and needles were used for high-throughput transcriptome sequencing. Compared with the reference genome of P. taeda, a total of 68 211 unigenes and 546 356 450 clean reads were obtained with an average mapping rate of 90.21%. The expression profiles between needles and xylem were compared in pairs. The differentially expressed genes (DEGs) were selected according to P＜0.05,|log2foldchange|> 1.0, and then annotated by GO and KEGG enrichment analysis. The results showed that 133 DEGs involved in terpene synthesis, most of which were enriched in MEP pathway. Eight candidate genes related to pine resin biosynthesis were selected from the DEGs and validated by RT-qPCR, in which HMGR, DXS, TPS, ABC transporter genes were highly correlated with the rosin production. Therefore, 133 differential genes related to pine resin biosynthesis were mined through transcriptome sequencing, and among which three TPS genes and two ABC transporter genes positively regulate terpenoid synthesis.
