Cloning and Expression Analysis of DELLA Genes in Camellia oleifera

Abstract:

To reveal the function and expression pattern of DELLA genes in Camellia oleifera, five DELLA genes, named CoDELLA1, CoDELLA2, CoDELLA3, CoDELLA4 and CoDELLA5, were cloned from 'Changlin 4' by PCR technique. The bioimformation of 5 encoding CoDELLA were analyzed, as well as expression patterns of 5 CoDELL and hormone response activities. The results showed that the CDS length of five CoDELLA genes were 1 791, 1 875, 1 848, 1 593 and 1 581 bp, encoding 597, 625, 616, 531 and 527 amino acids, respectively. The amino acid sequences of the five CoDELLA proteins were highly similar, and serine residues were the main potential phosphorylation sites. The N-terminal of CoDELLA proteins all contained typical DELLA domains. There were differences in the phylogeny of DELLA proteins from different species, CoDELLA had the highest homology with CsDELLA in C. sinensis. The expression of CoDELLA gene in different tissues was also different, which was regulated by various hormones and abiotic stresses such as gibberellin and aurolactone. Therefore, it was suggested that CoDELLA genes might play important role in the growth of C. oleifera and response to abiotic stress.

Key words:Camellia oleifera;DELLA;Gene;Bioinformatic;Gene expression