Abstract:In order to investigate the role of TCP transcription factors family of 3-year-old Magnolia sinostellata grafted seedlings under simulated natural shading, the bioinformation of TCP transcription factors were analyzed based on transcriptome data, as well as their expression patterns under shading stress. The results showed that 12 TCPs were identified from the transcriptome data of M. sinostellata, encoding 228-558 amino acids with protein size of 23.7-52.7 kDa. All of 12 TCPs proteins were unstable and hydrophilic. The 12 TCPs located in cell nucleus, and MsTCP12-a/b also located in chloroplast. MsTCPs could be divided into two types, Class I contained 10 TCP members, and Class II with two TCP members. All members contained TCP conserved domains and the closely TCP members had similar conserved motifs. Meanwhile, the TCP promoter sequences of M. sinostellata were cloned and analyzed, which contained a large number of light response elements and hormone response elements. The MsTCPs were differentially expressed under shading treatment. MsTCP9-a, MsTCP2 were significantly up-regulated and MsTCP8, MsTCP9-b, MsTCP7-a, MsTCP15 were significantly down-regulated. Therefore, MsTCPs might play an important role in response to shade stress.