Home > Archive>Volume 31, Issue 5, 2023 >660-666. DOI:10.11926/jtsb.4659
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Determination of Pollen Viability of Glycyrrhiza inflata and Hybridization Study of Licorice
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Department of Science and Technology of Guangzhou (Grant No. 202002030442). Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams, China (2020KJ148)

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    Abstract:

    Hybridization is one of the traditional methods to breed new cultivars, pollen viability is important for the successful transmission of genetic material to generations.Glycyrrhiza inflata is one of the three licorice species recorded in Chinese Pharmacopoeia, with many specialized active compounds. In order to determine the optimal pollen development periods for licorice hybridization, the pollen viability of five bloom stages (a, b, c, d, and e) of two G. inflata cultivars (GJJ-7 and GJJ-9) were measured by TTC staining and in vitro pollen germination methods. The result of TTC staining method showed that the pollen viability of GJJ-7 trended in a wave pattern and peaked at a and d stages. The pollen viability of GJJ-9 increased with the opening process of petals, and peaked at e stage. But the result of in vitro pollen germination method showed that the pollen viability of both GJJ-7 and GJJ-9 was highest at c stage, which was consistent with fruiting rate in the hybridization, and further indicating the higher reliability of the in vitro germination method compared with the TTC staining. Hybridization results of the three medicinal licorice species showed that a low fruiting rate but highest seed germination rate was obtained when G. glabra cultivars S-7 and S-12 were used as the female parents. High fruiting rate but low seed germination rate was observed when G. uralensis cultivar GDN-16 was used as the female parent, indicating the importance of parent selection during licorice hybridization. These provides an important reference for improving the field hybridization efficiency of licorice.

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谢姿雁,Botir KHAITOV,杨天顺,李重,李勇青,王瑛.胀果甘草花粉生活力测定及甘草的杂交育种[J].热带亚热带植物学报,2023,31(5):660~666

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History
  • Received:April 22,2022
  • Revised:April 25,2022
  • Adopted:July 19,2022
  • Online: September 26,2023
  • Published: September 20,2023
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