Home > Archive>Volume 30, Issue 5, 2022 >676-686. DOI:10.11926/jtsb.4494
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Correlation Analysis Between Single Nucleotide Polymorphism of ITS Sequences and Profile of Steroidal Saponins Composition of Paris polyphylla var.yunnanensis
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National Sciences Foundation of China (Grant No. 81573545); Technology Innovation and Research and Development Project of Chengdu Science and Technology Bureau (Grant No. 2019-YF05-00346-SN); Apricot Grove Scholars Program of Chengdu University of TCM(Grant No. QNXZ2018039)

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    Abstract:

    In order to understand the correlation between characters of single nucleotide polymorphism (SNP) of internal transcribed spacer (ITS) sequences and composition of steroidal saponins of Paris polyphylla var. yunnanensis, the effect of SNP on the quality stability of medicinal materials, the ITS sequences of 37 samples were compared by MegAlign, and then classified according to SNP loci. In addition, 7 steroidal saponins (polyphyllin Ⅰ, Ⅱ, Ⅴ, Ⅵ, Ⅶ, H, dioscin) in samples were determined by HPLC-DAD. The steroidal saponin composition characteristics of each genotype were statistical analysis by SPSS 25.0 & SIMCA-P 15.0. The results showed that there were 40 double-allelic polymorphic sites in ITS sequences of P. polyphylla var. yunnanensis, including 32 base conversion sites and 8 base inversion sites. According to SNP characteristics, 37 samples were divided into two genotypes: YN-I and YN-II. Six kinds of steroidal saponins (polyphyllin Ⅰ, Ⅱ, Ⅵ, Ⅶ, H, dioscin) were widely distributed in samples, while polyphyllin Ⅴ was relatively rare. The mean total content of pharmacopoeia index components in YN-I and YN-II were 1.070% and 0.93%, which the sample pass rate were 68.42% and 77.78%, respectively. The mean total content of 7 steroidal saponins were 1.65% and 1.32%, respectively. ANOVA analysis showed that there was significant differentiation of steroidal saponins profile between two genotypes, while there was no significant difference in the index components of pharmacopoeia, so the medicinal value of two genotypes should be equivalent. Cluster analysis (CA) and principal component analysis (PCA) showed the dispersion of YN-I was higher than that of YN-II, indicating the quality of medicinal materials of YN-II was more stable and the individual differences in steroidal saponins synthesis and accumulation in YN-II was smaller than YN-I. Therefore, the SNP characteristics of YN-II were of great significance for the screen of molecular genetic markers in the breeding of Paris polyphylla var. yunnanensis.

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尹鸿翔,张开元,任梓萱,赵家雯,蒋桂华,陈蓉.云南重楼ITS序列单核苷酸多态性与甾体皂苷特征的相关性分析[J].热带亚热带植物学报,2022,30(5):676~686

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History
  • Received:August 05,2021
  • Revised:November 05,2021
  • Adopted:June 09,2022
  • Online: September 26,2022
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