Abstract:To clarify the changes in volatile components of Dendrobium officinale, D. hercoglossum and their hybrid strains (14L-3, 14L-6, 14L-7 and 14L-9), their flowers were detected by static headspace combined with gas chromatography-mass spectrometry (GC-MS) technology. The results showed that there were 81 volatile components detected, including olefins, ketones, aldehydes, and alkanes. Among them, there are 23 compounds in D. officinale, 12 in D. hercoglossum, 21 in 14L-3, 33 in14L-6, 23 in 14L-7, and 35 in 14L-9. The main component in D. officinale flower was α-pinene, and that in D. hercoglossum was 2-pentadecanone. α-Pinene was the main component common in the four hybrids. The parents and hybrids contain hexanal. The hybrids 14L-3, 14L-6, and 14L-9 had high similarity with the female parent D. officinale in the order of 14L-3>14L-6>14L-9, they are quite different from the male parent D. hercoglossum. 14L-7 has the most balanced similarity with the parents. Therefore, these would provide guidance for breeding of Dendrobium.