Identification and Analysis of Superoxide Dismutase Gene Family Members of Cardamine hirsuta

Abstract:

In order to understand the characteristics of SOD gene in Cardamine hirsuta, the gene structure, chromosome location, phylogenetic relationship of SOD gene family were analyzed, the cis-acting element and protein structure were predicted, and the tissue expression patterns of the gene family numbers were measured by qRT-PCR. The results showed that a total of 10 SOD genes, named ChSODs, were identified from C. hirsuta genome, including 6 Cu/Zn-SOD, 3 Fe-SOD and 1 Mn-SOD. The amino acid numbers of ChSODs ranged from 57 to 324, with molecular weight from 6 419.41 to 34 659.01 kDa, and theoretical isoelectric point from 4.92 to 9.60. Phylogenetic tree showed that ChSOD had close relation with AtSOD of Arabidopsis. There were expression of ChSOD in root, stem and leaf, and the expression in leaves was high. Furthermore, the expression of CARHR085500 and CARHR256690 were high in leaves and stems. There were cis-acting elements responded to various abiotic stresses, in especial to ABA and low temperature stress. The secondary and tertiary structures of ChSODs were different. Therefore, these indicated that SOD gene of C. hirsuta play an important role in antioxidant process.

Key words:Cardamine hirsuta;SOD;Gene family