Cloning and Expression Analysis of SmVS Gene from Salvia miltiorrhiza

Abstract:

In order to understand the function of vinorine synthase gene (VS) in Salvia miltiorrhiza, the SmVS gene were cloned from S. miltiorrhiza, and the bioinformation of SmVS was predicted by Ex PASy and other online analysis software, the expression pattern of SmVS was analyzed by RT-qPCR. The results showed that the full-length of SmVS was 726 bp, encoding 241 amino acids. The molecular weight of SmVS was 26 861.72 with isoelectric point of 5.66. It was an extramembrane protein, presumably located in mitochondria. Phylogenetic tree showed that SmVS had close relationship with VS in Olea europaea var. sylvestris. The expression of SmVS in roots was higher than that in leaves, and the transcription level of SmVS reached the highest at 21:00 pm in leaves. Therefore, it was suggested that SmVS could be involve in responsing to light.

Key words:Salvia miltiorrhiza;Vinorine synthase;RT-qPCR;Gene expression;Clone