Home > Archive>Volume 29, Issue 6, 2021 >642-648. DOI:10.11926/jtsb.4404
PDF HTML XML Export Cite reminder
Molecular Marker Screening of Processed Pepper (Capsicum annuum) Germplasm Resources against with Phytophthora capsici in Chongqing
Author:
  • Article
    • | |
  • Metrics
    • |
  • Reference [32]
    • |
  • Related [20]
    • | | |
  • Comments
    Abstract:

    To screen out molecular markers suitable for identification of Phytophthora capsici resistant pepper in Chongqing, the screening efficiency of 12 molecular makers for Phytophthora blight resistance was studied in 63 processed pepper (Capsicum annuum) germplasms in Chongqing. The results showed that there were no different amplification bands of 7 markers among the pepper germplasms, and the amplification results of 2 markers were unstable. Only 3 markers, such as ZL6203, ZL6726 and E73, could amplify different bands among pepper germplasms. For ZL6203, the efficiency of screening high resistant, resistant and medium resistant materials was 87.50%, 77.78% and 63.64%, respectively. For ZL6726, the efficiency of screening resistant and susceptible materials was 100.00% and 66.67%, respectively, and for E73, the efficiency of screening resistant and high resistant materials was 77.78% and 62.50%, respectively. Therefore, the screening efficiency of the same molecular marker for different resistance materials was quite different. ZL6203, ZL6726 and E73 markers were suitable for identification of Phytophthora blight resistant of processed pepper in Chongqing.

    Reference
    [1] SHAO Y J, WU W W, SHEN S X, et al. Genetic study on genic male sterility of pepper and its application in hybrid breeding[J]. J Trop Subtrop Bot, 2013, 21(1):93-99. doi:10.3969/j.issn.1005-3395.2013.01.014.邵元健, 吴雯雯, 沈素香, 等. 辣椒雄性核不育基因的遗传研究及其在杂交育种中的应用[J]. 热带亚热带植物学报, 2013, 21(1):9399. doi:10.3969/j.issn.1005-3395.2013.01.014.
    [2] ZHANG Q Q, LIANG S, WANG Y, et al. Genetic diversity analysis of 57 germplasms of Capcicum annuum based on phenotypic traits and SSR markers[J]. J Trop Subtrop Bot, 2020, 28(4):356-366. doi:10. 11926/jtsb.4185.张强强, 梁赛, 王艳, 等. 基于表型性状和SSR标记的57份辣椒种质遗传多样性分析[J]. 热带亚热带植物学报, 2020, 28(4):356-366. doi:10.11926/jtsb.4185.
    [3] BARCHENGER D W, LAMOUR K H, BOSLAND P W. Challenges and strategies for breeding resistance in Capsicum annuum to the multifarious pathogen, Phytophthora capsici[J]. Front Plant Sci, 2018, 9:628. doi:10.3389/fpls.2018.00628.
    [4] FANG Z D, LU J Y, YE Z Y. Chinese Agricultural Encyclopedia, Volume Plant Pathology[M]. Beijing:China Agricultural Publishing House, 1996:274-275.方中达, 陆家云, 叶钟音. 中国农业百科全书:植物病理学卷[M]. 北京:中国农业出版社, 1996:274-275.
    [5] JIA J S. On the chilli pepper Phytophora blight and it's control in Xinjiang[J]. Acta Phytopathol Sin, 1992, 22(3):257-263. doi:10. 13926/j.cnki.apps.1992.03.021.贾菊生. 新疆辣椒疫病及防治研究[J]. 植物病理学报, 1992, 22(3):257-263. doi:10.13926/j.cnki.apps.1992.03.021.
    [6] CHANG C T, GONG Z H, WANG M. Studies on the pathogen of pepper Phytophthora blight in Shanxi[J]. Acta Agric Boreali-Occid Sin, 1993, 2(1):87-90.常彩涛, 巩振辉, 王鸣. 陕西辣椒疫病菌种的鉴定[J]. 西北农业学报, 1993, 2(1):87-90.
    [7] XIAO S Q, LU X H, LIU T R. Investigation on pepper blight of Heilongjiang Province[J]. Heilongjiang Agric Sci, 2002(5):45-46. doi:10.3969/j.issn.1002-2767.2002.05.017.肖淑芹, 陆秀华, 刘惕若. 黑龙江省辣椒疫病发生情况调查[J]. 黑龙江农业科学, 2002(5):45-46. doi:10.3969/j.issn.1002-2767.2002.05.017.
    [8] YANG X H, YUAN J, XIE H C. Identification on Phytophthora blight resistance in major cultivars of pepper in Guizhou Province[J]. SW China J Agric Sci, 2005, 18(6):791-793. doi:10.3969/j.issn.10014829.2005.06.027.杨学辉, 袁洁, 谢海呈. 贵州省辣椒主栽品种抗疫病性鉴定[J]. 西南农业学报, 2005, 18(6):791-793. doi:10.3969/j.issn.1001-4829. 2005.06.027.
    [9] ZHANG S C, LÜ Z H, HUANG R Z, et al. Integrated control against major diseases of processing-purpose hot pepper in Chongqing[J]. J China Capsi, 2012(3):33-34,36. doi:10.3969/j.issn.1672-4542.2012.03.010.张世才, 吕中华, 黄任中, 等. 重庆加工型辣椒主要病害的综合防治技术[J]. 辣椒杂志, 2012(3):33-34,36. doi:10.3969/j.issn.16724542.2012.03.010.
    [10] LI Z J, LONG W P, ZHENG J R, et al. Isolation and identification of Phytophthora capsici in Guangdong province and measurement of their pathogenicity and physiological race differentiation[J]. J S China Agric Univ, 2007, 28(1):50-54. doi:10.3969/j.issn.1001-411X.2007.01.012.李智军, 龙卫平, 郑锦荣, 等. 广东辣椒疫霉菌分离鉴定及其致病力和生理小种分化研究[J]. 华南农业大学学报, 2007, 28(1):50-54. doi:10.3969/j.issn.1001-411X.2007.01.012.
    [11] ZHAI M T, WANG K Y, XU H, et al. Induction and charcteristics of Phytophthora capsici isolates resistant to fluopicolide[J]. Acta Phytopathol Sin, 2014, 44(1):88-96. doi:10.13926/j.cnki.apps.2014.01.010.翟明涛, 王开运, 许辉, 等. 抗氟吡菌胺辣椒疫霉菌株的诱导及其生物学特性的研究[J]. 植物病理学报, 2014, 44(1):88-96. doi:10. 13926/j.cnki.apps.2014.01.010.
    [12] FU Y B, YANG M H, ZENG F Q, et al. Searching for an accurate marker-based prediction of an individual quantitative trait in molecular plant breeding[J]. Front Plant Sci, 2017, 8:1182. doi:10.3389/fpls. 2017.01182.
    [13] LI Y F, ZHANG S C, JIANG X Y, et al. Creation of new germplasm with Phytophthora blight resistance for processing type pepper by anther culture[J]. Mol Plant Breed, 2019, 17(12):4030-4035. doi:10. 13271/j.mpb.017.004030.李怡斐, 张世才, 蒋晓英, 等. 利用花药培养技术创制加工型辣椒抗疫病新种质[J]. 分子植物育种, 2019, 17(12):4030-4035. doi:10. 13271/j.mpb.017.004030.
    [14] WANG L H, ZHANG B X, ZHANG Z H, et al. Research progress in genetics and breeding of Capsicum[J]. Acta Hort Sin, 2020, 47(9):1727-1740. doi:10.16420/j.issn.0513-353x.2020-0487.王立浩, 张宝玺, 张正海, 等. 辣椒遗传育种研究进展[J]. 园艺学报, 2020, 47(9):1727-1740. doi:10.16420/j.issn.0513-353x.2020-0487.
    [15] KIM N, KANG W H, LEE J, et al. Development of clustered resistance gene analogs-based markers of resistance to Phytophthora capsici in Chili pepper[J]. BioMed Res Int, 2019, 2019:1093186. doi:10.1155/2019/1093186.
    [16] PARISI M, ALIOTO D, TRIPODI P. Overview of biotic stresses in pepper (Capsicum spp.):Sources of genetic resistance, molecular breeding and genomics[J]. Int J Mol Sci, 2020, 21(7):2587. doi:10. 3390/IJMS21072587.
    [17] HUANG R Z, HUANG Q Z, LÜ Z H, et al. Present situation and development of countermeasures of dry pepper industry in China[J]. China Veget, 2015, 1(2):9-11. doi:10.3969/j.issn.1000-6346.2015.02.004.黄任中, 黄启中, 吕中华, 等. 我国干制辣椒产业现状及发展对策[J]. 中国蔬菜, 2015, 1(2):9-11. doi:10.3969/j.issn.1000-6346.2015.02.004.
    [18] ZHANG S C, XIONG Y, HUANG R Z, et al. Isolation and cultivation of Phytophthora capsici and identification of physiological race in Chongqing[J]. Plant Protec, 2015, 41(3):183-187. doi:10.3969/j.issn. 0529-1542.2015.03.036.张世才, 熊艳, 黄任中, 等. 重庆地区辣椒疫霉菌的分离培养及生理小种鉴定[J]. 植物保护, 2015, 41(3):183-187. doi:10.3969/j.issn. 0529-1542.2015.03.036.
    [19] LI Y X, GONG Z H, LI D W, et al. Development and utilization of a co-dominant RGA-STS marker associated with resistance to Phytophthora capsici in pepper[J]. Acta Agric Boreali-Occid Sin, 2011, 20(6):123-127. doi:10.3969/j.issn.1004-1389.2011.06.027.李永新, 巩振辉, 李大伟, 等. 辣椒疫病抗性相关的共显性RGASTS标记的开发及应用[J]. 西北农业学报, 2011, 20(6):123-127. doi:10.3969/j.issn.1004-1389.2011.06.027.
    [20] WANG D Y, AN K, LI Y, et al. A molecular marker-assisted selection method for anti-epidemic breeding of pepper:CN, 100447254C[P]. 2008-12-31.王得元, 安康, 李颖, 等. 一种辣椒抗疫病育种的分子标记辅助选择方法:中国, 100447254C[P]. 2008-12-31.
    [21] GONG Z H, LIU K K, LU M H, et al. A method of eliminating breeding materials of pepper sensory disease by molecular markers:CN101487049B[P]. 2011-04-06.巩振辉, 刘珂珂, 逯明辉, 等. 一种利用分子标记淘汰辣椒感疫病育种材料的方法:中国101487049B[P]. 2011-04-06.
    [22] QUIRIN E A, OGUNDIWIN E A, PRINCE J P, et al. Development of sequence characterized amplified region (SCAR) primers for the detection of Phyto.5.2, a major QTL for resistance to Phytophthora capsici Leon. in pepper[J]. Theor Appl Genet, 2005, 110(4):605-612. doi:10.1007/s00122-004-1874-7.
    [23] ZHANG X F, HAN H L, CHEN B, et al. Genetic and molecular marker analysis of resistance to Phythophtora capsici in sweet pepper[J]. Acta Hort Sin, 2011, 38(7):1325-1332. doi:10.16420/j.issn.0513-353x. 2011.07.017.张晓芬, 韩华丽, 陈斌, 等. 甜椒疫病抗性遗传及相关基因分子标记研究[J]. 园艺学报, 2011, 38(7):1325-1332. doi:10.16420/j.issn. 0513-353x.2011.07.017.
    [24] SUGITA T, YAMAGUCHI K, KINOSHITA T, et al. QTL analysis for resistance to Phytophthora blight (Phytophthora capsici Leon.) using an intraspecific doubledhaploid population of Capsicum annuum[J]. Breed Sci, 2006, 56(2):137-145. doi:10.1270/jsbbs.56.137.
    [25] WANG P Y, WANG L R, GUO J J, et al. Molecular mapping of a gene conferring resistance to Phytophthora capsici Leonian race 2 in pepper line PI201234(Capsicum annuum L.)[J]. Mol Breed, 2016, 36(6):66. doi:10.1007/S11032-016-0464-0.
    [26] GUO S, HUANG Z, CHANG S D, et al. Identification of resistance to Phytophthora blight in hot pepper using molecular marker[J]. Chin Agric Sci Bull, 2012, 28(13):163-166. doi:10.3969/j.issn.1000-6850. 2012.13.030.郭爽, 黄贞, 常绍东, 等. 利用分子标记鉴定辣椒抗疫病材料[J]. 中国农学通报, 2012, 28(13):163-166. doi:10.3969/j.issn.1000-6850. 2012.13.030.
    [27] MA S B, SUN Y, WANG C, et al. Molecular marker assisted selection of pepper materials with resistance to Phytophthora blight[J]. N Hort, 2013(10):107-110.马寿宾, 孙艳, 王琛, 等. 分子标记辅助选择辣椒抗疫病新种质研究[J]. 北方园艺, 2013(10):107-110.
    [28] ZHANG A M, PENG G H, LIANG C J, et al. Molecular marker primers screening of identification on local pepper with Phytophthora blight resistance in Guizhou[J]. Guizhou Agric Sci, 2017, 45(11):1014. doi:10.3969/j.issn.1001-3601.2017.11.004.张爱民, 蓬桂华, 梁传静, 等. 贵州地方辣椒材料疫病抗性鉴定的分子标记引物筛选[J]. 贵州农业科学, 2017, 45(11):10-14. doi:10. 3969/j.issn.1001-3601.2017.11.004.
    [29] RETES-MANJARREZ J E, RUBIO-ARAGÓN W A, MÁRQUESZEQUERA I, et al. Novel sources of resistance to Phytophthora capsici on pepper (Capsicum sp.) landraces from Mexico[J]. Plant Pathol J, 2020, 36(6):600-607. doi:10.5423/PPJ.OA.07.2020.0131.
    [30] BARKSDALE T H, PAPAVIZAS G C, JOHNSTON S A. Resistance to foliar blight and crown rot of pepper caused by Phytophthora capsici[J]. Plant Dis, 1984, 68(6):506-509. doi:10.1094/PD-68-506.
    [31] THABUIS A, PALLOIX A, PFLIEGER S, et al. Comparative mapping of Phytophthora resistance loci in pepper germplasm:Evidence for conserved resistance loci across Solanaceae and for a large genetic diversity[J]. Theor Appl Genet, 2003, 106(8):1473-1485. doi:10. 1007/s00122-003-1206-3.
    [32] LEFEBVRE V, PALLOIX A. Both epistatic and additive effects of QTLs are involved in polygenic induced resistance to disease:A case study, the interaction pepper-Phytophthora capsici Leonian[J]. Theor Appl Genet, 1996, 93(4):503-511. doi:10.1007/BF00417941.
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

王春萍,杨小苗,李怡斐,雷开荣,黄启中,黄任中,林清,吴红,张世才.重庆加工型辣椒种质资源抗疫病鉴定的分子标记筛选[J].热带亚热带植物学报,2021,29(6):642~648

Copy
Share
Article Metrics
  • Abstract:309
  • PDF: 477
  • HTML: 429
  • Cited by: 0
History
  • Received:February 28,2021
  • Revised:May 13,2021
  • Online: December 02,2021
Article QR Code
You are the first4646429 Visitors
Governing Body：中国科学院
Organizer：中国科学院华南植物园 广东省植物学会
Address：Xingke Road 723,Tianhe District,Guangzhou,China
Phone：+86 (0)20-3725 2514
Journal of Tropical and Subtropical Botany ® 2025 All Rights Reserved