Abstract:In order to explore the effect of NAA on cutting rooting of Blumea balsamifera, the changes in physiological and biochemical characteristics of 4-year-old healthy branches as cuttings were studied treated with 500 mg/L NAA. The results showed that rooting rate was positively correlated with endogenous IAA, GA content and IAA/ABA, but negatively with ABA content. The IAA content of cuttings increased and ABA content decreased treated with NAA, showing that NAA had helped with adventitious roots. The rooting rate of cuttings was positively correlated with PPO activity and negatively with IAAO activity, and NAA could enhance PPO activity and reduce IAAO activity of cuttings, which is beneficial to rooting. The contents of soluble protein and total soluble sugar were positively correlated with rooting rate. NAA could promote the accumulation of total soluble sugar and soluble protein, improve the cell differentiation and metabolic ability of cuttings. Therefore, 500 mg/L NAA could promote the growth and development of adventitious roots.