Bioactivity and Chemical Composition of Ethanol Extract from Michelia champaca Leaves
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    Abstract:

    In order to excavate the development and utilization potential of Michelia champaca leaves, the bioactivity and chemical composition of ethanol extract from leaves obtained by ultrasonic extraction were studied. The results showed that 20% and 80% ethanol extracts had antioxidant potential ability by using DPPH∙ free radical scavenging assay and reducing power of iron ion. There was cytotoxicity to HacaT cells, when the concentration of 20% or 80% extracts was more than 12.5 or 6.25 μg/mL, respectively. A total of 206 compounds were obtained from ethanol extract from M. champaca leaves by UPLC-MS combined with software spectrum library, including flavonoids, alkaloids, fatty acids, alcohols and so on. Among the flavonoids, rutin, quercetin and kaempferol could be identified clearly by standard product verification. The contents of compounds in ethanol extracts of M. champaca leaves were different among different months, and the distribution of components in spring and summer were significantly different from that in autumn and winter. Besides, some compositions had seasonal characteristics. In conclusion, Michelia champaca leaves had potential in the development of daily chemical products, and the harvest time could be selected according to the actual composition.

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    [1] Delectis Florae Reipublicae Popularis Sinicae, Agendae Academiae Sinicae Edita. Florae Reipublicae Popularis Sinicae, Tomus 30(1)[M]. Beijing:Science Press, 1996:157. 中国科学院中国植物志编辑委员会. 中国植物志, 第30卷第1分册[M]. 北京:科学出版社, 1996:157.
    [2] SUJARWO W, KEIM A P. Ethnobotanical study of traditional building materials from the island of Bali, Indonesia[J]. Econ Bot, 2017, 71(3):224-240. doi:10.1007/s12231-017-9385-z.
    [3] Liu S L, Kao C L, Yeh H C, et al. A new α-ionone from Michelia champaca[J]. Chem Nat Compd, 2020, 56:389-391. doi:10.1007/s 10600-020-03043-w.
    [4] DEVATH C P, THALLA A K, KATTE S Y. Green synthesis of iron nanoparticles using different leaf extracts for treatment of domestic waste water[J]. J Clean Prod, 2016, 139:1425-1435. doi:10.1016/j. jclepro.2016.09.019.
    [5] ANANTHI T, CHITRA M. In vitro evaluation of antioxidant activity of Michelia champaca (L.) flowers[J]. Amer J Adv Drug Deliv, 2013, 1(5):734-742.
    [6] TAPRIAL S. A review on phytochemical and pharmacological properties of Michelia champaca Linn. family:Magnoliaceae[J]. Int J Pharm, 2015, 2(9):430-436. doi:10.13040/IJPSR.0975-8232.IJP.2(9). 430-36.
    [7] HOSAMANI K M, HIREMATH V B, KERI R S. Renewable energy sources from Michelia champaca and Garcinia indica seed oils:A rich source of oil[J]. Biom Bioenerg, 2009, 33(2):267-270. doi:10.1016/j.biombioe.2008.05.010.
    [8] SRIVASTAVA R C, SINGH R K, COMMUNITY A, et al. Indigenous biodiversity of Apatani plateau:Learning on biocultural knowledge of Apatani tribe of Arunachal Pradesh for sustainable livelihoods[J]. Ind J Trad Know, 2010, 9(3):432-442.
    [9] BHUPATHYRAAJ M, KUMAR M S. Effect of Michelia champaca Linn. on pylorous ligated rats[J]. J Appl Pharm Sci, 2011, 1(2):60-64.
    [10] ANANTHI T, KALAISELVI R. FT-IR analysis and in vitro anti-inflammatory activity of leaf extract of Michelia champaca L.[J]. World J Pharm Res, 2016, 5(6):1263-1272.
    [11] GUPTA S, MEHLA K, CHAUHAN D, et al. Anti-inflammatory activity of leaves of Michelia champaca investigated on acute inflam-mation induced rats[J]. Lat Am J Pharm, 2011, 30(4):819-822.
    [12] MANHAS N, DAHIYA P. In vitro antimicrobial activity and phyto-chemical screening of leaf and stem extracts of Michelia champaca Linn.[J]. Int Food Res J, 2017, 24(6):2672-2676.
    [13] DU Y X, ZHANG J W, HU Z H, et al. Optimization of ultrasonic extraction process of total flavonoids from leaves of Ilex centro-chinensis S. Y. Hu and its bacterioststic effect[J]. Chin Trad Pat Med, 2019, 41(9):2200-2203. 杜银香, 张建伟, 胡泽华, 等. 华中枸骨叶总黄酮超声提取工艺的优化及其抑菌作用[J]. 中成药, 2019, 41(9):2200-2203.
    [14] XIANG S, CHEN Z M, ZHANG X H, et al. Extraction of total flavonoids from the leaves of Euscaphis konishli Hayata[J]. Wuyi Sci J, 2018, 34(1):118-122. doi:10.15914/j.cnki.wykx.2018.34.17. 向双, 陈泽明, 张晓华, 等. 圆齿野鸦椿叶片总黄酮提取工艺的研究[J]. 武夷科学, 2018, 34(1):118-122. doi:10.15914/j.cnki.wykx. 2018.34.17.
    [15] YANG D. Chemical constituents of strawberry cv. Falandi fruit and starfruit and their bioactivities[D]. Beijing:University of Chinese Academy of Sciences, 2015:81-83. 杨丹. 草莓和杨桃果实的化学成分及其生物活性研究[D]. 北京:中国科学院大学, 2015:81-83.
    [16] XU X Y, XIE H H, HAO J, et al. Eudesmane sesquiterpene glucosides from lychee seed and their cytotoxic activity[J]. Food Chem, 2010, 123(4):1123-1126. doi:10.1016/j.foodchem.2010.05.073.
    [17] MO C L. The proliferation and apoptosis effect of erianin on HacaT cells and the study of dendritic mesoporous silica nanosphere on skin delivery[D]. Guangzhou:South China University of Technology, 2020:35-37. 莫灿龙. 毛兰素对角质细胞增殖和凋亡的影响及树状介孔硅透皮给药系统的研究[D]. 广州:华南理工大学, 2020:35-37.
    [18] LI C, HU Q, ZHANG Y X, et al. Research progress on pharmaco-logical activity of quercetin[J]. Hubei J Trad Chin Med, 2018, 40(6):63-66. 李聪, 胡强, 张燕翔, 等. 槲皮素的药理学活性研究进展[J]. 湖北中医杂志, 2018, 40(6):63-66.
    [19] PARVIN K, HASANUZZAMAN M, BHUYAN M H M B, et al. Quercetin mediated salt Tolerance in tomato through the enhancement of plant antioxidant defense and glyoxalase systems[J]. Plants, 2019, 8(8):247. doi:10.3390/plants8080247.
    [20] DIAS M C, PINTO D C G A, FIGUEIREDO C, et al. Phenolic and lipophilic metabolite adjustments in Olea europaea (olive) trees during drought stress and recovery[J]. Phytochemistry, 2021, 185:112695. doi:10.1016/j.phytochem.2021.112695.
    [21] JIANG L H, LIU H F, HAO G F, et al. Research progress on anti-oxidant ability of astaxanthin[J]. Sci Technol Food Ind, 2019, 40(10):350-354. doi:10.13386/j.issn1002-0306.2019.10.057. 江利华, 柳慧芳, 郝光飞, 等. 虾青素抗氧化能力研究进展[J]. 食品工业科技, 2019, 40(10):350-354. doi:10.13386/j.issn1002-0306. 2019.10.057.
    [22] LIN Z, WANG J X, CHEN Q Y, et al. Thermal stability of antioxidant property in aqueous extract of Piper sarmentosum Roxb. leaf[J]. J Food Saf Qual, 2020, 11(4):1134-1140.  林震, 王嘉欣, 陈秋莹, 等. 蛤蒌叶水提取物抗氧化活性的热稳定性研究[J]. 食品安全质量检测学报, 2020, 11(4):1134-1140.
    [23] NIU X Q, DU L J, LIN X X, et al. Components of essential oils from Zanthoxylum piperitum leaves and its capacities of antioxidant, anthel-mintic, and bacteriostasis[J]. J Trop Subtrop Bot, 2016, 24(1):93-98. doi:10.11926/j.issn.1005-3395.2016.01.013. 牛先前, 杜丽君, 林秀香, 等. 胡椒木叶片精油成分分析及其抗氧化、驱虫、抗菌活性[J]. 热带亚热带植物学报, 2016, 24(1):93-98. doi:10.11926/j.issn.1005-3395.2016.01.013.
    [24] LIU Y H, XIA N H, YANG H Q. The origin, evolution and phyto-geography of Magnoliaceae[J]. J Trop Subtrop Bot, 1995, 3(4):1-12. 刘玉壶, 夏念和, 杨惠秋. 木兰科(Magnoliaceae)的起源、进化和地理分布[J]. 热带亚热带植物学报, 1995, 3(4):1-12.
    [25] Tan L H, Zhang D, Wang G, et al. Comparative analyses of flavonoids compositions and antioxidant activities of Hawk tea from six botanical origins[J]. Ind Crops Prod, 2016, 80:123-130. doi:10. 1016/j.indcrop.2015.11.035.
    [26] Xue Y L, AHIKO T, MIYAKAWA T, et al. Isolation and Caenor-habditis elegans lifespan assay of flavonoids from onion[J]. J Agric Food Chem, 2011, 59(11):5927-5934. doi:10.1021/jf104798n.
    [27] CHO S Y, KIM H W, LEE M K, et al. Antioxidant and anti-inflammatory activities in relation to the flavonoids composition of pepper (Capsicum annuum L.)[J]. Antioxidants, 2020, 9(10):E986. doi:10.3390/antiox9100986.
    [28] HAN Y W, WANG C C, LI X M. Protective effect of rutin on early brain injury in mice with subarachnoid hemorrhage by anti-oxidation and anti-apoptosis[J]. Drugs Clin, 2020, 35(3):421-425. doi:10.7501/j.issn.1674-5515.2020.03.006. 韩雨薇, 王晨辰, 李晓明. 芦丁通过抗氧化抗凋亡对蛛网膜下腔出血大鼠早期脑损伤的保护作用[J]. 现代药物与临床, 2020, 35(3):421-425. doi:10.7501/j.issn.1674-5515.2020.03.006.
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彭思敏,杨小满,魏孝义,潘丽珠,王瑛.黄兰叶乙醇提取物的活性研究和成分分析[J].热带亚热带植物学报,2022,30(1):135~143

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  • Received:March 01,2021
  • Revised:April 30,2021
  • Online: January 27,2022
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