Home > Archive>Volume 29, Issue 5, 2021 >539-546. DOI:10.11926/jtsb.4337
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Genetic Diversity of Archidendron clypearia in Guangdong Province
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    Abstract:

    In order to understand the genetic diversity of Archidendron clypearia germplasms resources, the genetic diversity and relatedness of 146 individuals from 12 wild populations in Guangdong Province were studied by using SSR molecular marker technology. The results showed that a total of 249 alleles were detected by 21 pairs of SSR primers with an average alleles (Na) of 11.857, the effective number of alleles (Ne) of 3.500, as well as expected heterozygosity (He) of 0.718, and polymorphism information content (PIC) of 0.676. The Shannon diversity index (I=0.528) and effective number of alleles (Ne=0.716) of Boluo population were the highest among 12 populations, which is the most genetically diverse population. The coefficient of genetic differentiation among populations was 0.071, and the genetic variation of A. clypearia was mainly within populations (97%), and the genetic differentiation within populations was greater than that among populations. Meanwhile, the cluster analysis showed that the 12 populations could be divided into 6 types according to genetic coefficient of 0.16, which was similar to that of principal coordinate analysis. So, these would provide an important theoretical basis for the exploitation, utilization, and protection of A. clypearia germplasm resources, and genetic breeding of fine variety.

    Reference
    [1] WU D L. Flora of Guangdong, Vol. 5[M]. Guangzhou:Guangdong Science and Technology Press, 2003:60-80.吴德邻. 广东植物志, 第5卷[M]. 广东:广东科技出版社, 2003:60-80.
    [2] ZHENG W J. China Annals of Trees, Vol. 2[M]. Beijing:China Forestry Publishing House, 1985:2235-2337.郑万钧. 中国树木志, 第2卷[M]. 北京:中国林业出版社, 1985:2235-2337.
    [3] LI Y L, LI K M, SU M X, et al. Studies on antiviral constituents in stems and leaves of Pithecellibium clypearia[J]. China J Chin Mat Med, 2006, 31(5):397-400. doi:10.3321/j.issn:1001-5302.2006.05.013.李药兰, 李克明, 苏妙贤, 等. 猴耳环抗病毒有效成分研究[J]. 中国中药杂志, 2006, 31(5):397-400. doi:10.3321/j.issn:1001-5302. 2006.05.013.
    [4] LI M H, YIN M Q, FENG J H, et al. Advances in antitumor effects of gallic acid[J]. Inform Trad Chin Med, 2011, 28(1):109-111. doi:10. 3969/j.issn.1002-2406.2011.01.044.李沐涵, 殷美琦, 冯靖涵, 等. 没食子酸抗肿瘤作用研究进展[J]. 中医药信息, 2011, 28(1):109-111. doi:10.3969/j.issn.1002-2406.2011. 01.044.
    [5] KANG J, LIU C, WANG H Q, et al. Studies on the bioactive flavor-noids isolated from Pithecellobium clypearia Benth.[J]. Molecules, 2014, 19(4):4479-4490. doi:10.3390/molecules19044479.
    [6] CAI X A, PENG S L, CAO H L, et al. Community analysis of a secondary evergreen broadleaved forest at Luogang, Guangzhou[J]. Chin J Appl Environ Biol, 1998, 4(2):107-114.蔡锡安, 彭少麟, 曹洪麟, 等. 广州罗岗村边次生常绿阔叶林群落分析[J]. 应用与环境生物学报, 1998, 4(2):107-114.
    [7] LI M, HUANG S N, CHEN Z X, et al. The research status and utili-zation prospect of medicinal tree species of Archidendron clypearia[J]. Sci Silv Sin, 2018, 54(4):142-154. doi:10.11707/j.1001-7488.20180417.李梅, 黄世能, 陈祖旭, 等. 药用乔木树种猴耳环研究现状及开发利用前景[J]. 林业科学, 2018, 54(4):142-154. doi:10.11707/j.1001-7488.20180417.
    [8] ZIETKIEWICZ E, RAFALSKI A, LABUDA D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20(2):176-183. doi:10.1006/geno. 1994.1151.
    [9] HU W S, DENG C J, XU Q Z, et al. Identification and fingerprint construction of 19 new hybrid varieties (lines) of loquat by SSR[J]. J Trop Subtrop Bot, 2020, 28(2):153-162. doi:10.11926/jtsb.4131.胡文舜, 邓朝军, 许奇志, 等. 19个枇杷杂交新品种(系)的SSR鉴定和指纹图谱构建[J]. 热带亚热带植物学报, 2020, 28(2):153-162. doi:10.11926/jtsb.4131.
    [10] WANG L, LI C R, LI F G, et al. Analysis of SSR marker diversity and population structure in Eucalyptus cloeziana[J]. Mol Plant Breed, 2019, 17(13):4470-4478. doi:10.13271/j.mpb.017.004470.王莉, 李昌荣, 李发根, 等. 大花序按SSR位点多样性和群体结构分析[J]. 分子植物育种, 2019, 17(13):4470-4478. doi:10.13271/j. mpb.017.004470.
    [11] ZHU Q, DENG X, ZHANG S B, et al. Genetic diversity of 6 species in Polygonatum by SSR marker[J]. China J Chin Mat Med, 2018, 43(14):2935-2943. doi:10.19540/j.cnki.cjcmm.20180510.014.朱巧, 邓欣, 张树冰, 等. 黄精属6种植物的SSR遗传差异分析[J]. 中国中药杂志, 2018, 43(14):2935-2943. doi:10.19540/j.cnki.cjcmm. 20180510.014.
    [12] WANG G, CAO P, WEI X M, et al. Applications of molecular markers in study of germplasm resources of medicinal plants[J]. Mod Chin Med, 2019, 21(11):1435-1444. doi:10.13313/j.issn.1673-4890.20190830006.王刚, 曹佩, 韦学敏, 等. 分子标记技术在药用植物种质资源研究中的应用[J]. 中国现代中药, 2019, 21(11):1435-1444. doi:10.13313/j. issn.1673-4890.20190830006.
    [13] ZHAO J H. Molecular identification and chemical component analysis of Archidendron dypearia from different regions in China[D]. Guang-zhou:Guangzhou University of Chinese Medicine, 2013:16-30.赵径华. 不同产地猴耳环分子鉴别及质量研究[D]. 广州:广州中医药大学, 2013:16-30.
    [14] LI R H, XIA Y S, LIU S Z, et al. CTAB-impoved method of DNA extraction in Plant[J]. Res Explor Lab, 2009, 28(9):14-16. doi:10. 3969/j.issn.1006-7167.2009.09.005.李荣华, 夏岩石, 刘顺枝, 等. 改进的CTAB提取植物DNA方法[J]. 实验室研究与探索, 2009, 28(9):14-16. doi:10.3969/j.issn.1006-7167. 2009.09.005.
    [15] HOLLAND M M, PARSON W. GeneMarker® HID:A reliable software tool for the analysis of forensic STR data[J]. J Forensic Sci, 2011, 56(1):29-35. doi:10.1111/j.1556-4029.2010.01565.x.
    [16] KALINOWSKI S T, TAPER M L, MARSHALL T C. Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment[J]. Mol Ecol, 2007, 16(5):1099-1106. doi:10.1111/j.1365-294X.2007.03089.x.
    [17] PEAKALL R, SMOUSE P E. GENALEX 6:Genetic analysis in Excel:Population genetic software for teaching and research[J]. Mol Ecol Notes, 2006, 6(1):288-295. doi:Notes10.1111/j.1471-8286.2005.01155.x.
    [18] ROHLF F J. NTSYS-pc:Numerical Taxonomy and Multivariate Analysis System Version 2.1[M]. New York:Exeter Publications, 2000:236-239.
    [19] XU N, CHENG X Z, WANG L X, et al. Screening and application of SSR molecular markers for genetic diversity analysis of Chinese Adzuki bean germplasm resources[J]. Acta Agron Sin, 2009, 35(2):219-227. doi:10.3724/SP.J.1006.2009.00219.徐宁, 程须珍, 王丽侠, 等. 用于中国小豆种质资源遗传多样性分析SSR分子标记筛选及应用[J]. 作物学报, 2009, 35(2):219-227. doi:10.3724/SP.J.1006.2009.00219.
    [20] ZHANG X F, ZHANG S H, LI Y, et al. Genetic diversity analysis of chestnut germplasm in Yanshan region based on SSR markers[J]. J Chin Agric Univ, 2020, 25(4):61-71. doi:10.11841/j.issn.1007-4333. 2020.04.07.张馨方, 张树航, 李颖, 等. 基于SSR标记的燕山板栗种质资源遗传多样性分析[J]. 中国农业大学学报, 2020, 25(4):61-71. doi:10. 11841/j.issn.1007-4333.2020.04.07.
    [21] NYBOM H. Comparison of different nuclear DNA markers for esti-mating intraspecific genetic diversity in plants[J]. Mol Ecol, 2004, 13(5):1143-1155. doi:10.1111/j.1365-294X.2004.02141.x.
    [22] HAMRICK J L. Isozymes and the analysis of genetic structure in plant populations[M]//SOLTIS D E, SOLTIS P S. Isozymes in Plant Biology. London:Chapman and Hall, 1990:87-105
    [23] LI H, HUANG S N, WANG W W, et al. Effects of different solar-shading treatments on growth performance of Archidendron clypearia seedlings[J]. J CS Univ For Technol, 2012, 32(10):147-150,197. doi:10.14067/j.cnki.1673-923x.2012.10.018.李浩, 黄世能, 王卫文, 等. 不同遮阳处理对猴耳环苗期生长的影响[J]. 中南林业科技大学学报, 2012, 32(10):147-150,197. doi:10. 14067/j.cnki.1673-923x.2012.10.018.
    [24] HU W, LUO Y, YANG Y, et al. Genetic diversity and population genetic structure of wild sweet osmanthus revealed by microsatellite markers[J]. Acta Hort Sin, 2014, 41(7):1427-1435. doi:10.16420/j. issn.0513-353x.2014.07.018.胡菀, 罗意, 阳亿, 等. 野生桂花的遗传多样性和遗传结构研究[J]. 园艺学报, 2014, 41(7):1427-1435. doi:10.16420/j.issn.0513-353x. 2014.07.018.
    [25] WRIGHT S. Evolution and the Genetics of Populations, Volume 4:Variability within and among Natural Populations[M]. Chicago:University of Chicago Press, 1978:1191-1192.
    [26] HUANG Z H. Feeding ecology of Françoisi langurs (Trachypithecus françoisi) in Nonggang Nature Reserve, China[D]. Guilin:Guangxi Normal University, 2007:13-32.黄中豪. 弄岗黑叶猴(Trachypithecus françoisi)的觅食生态学[D]. 桂林:广西师范大学, 2007:13-32.
    [27] ZHOU Q H, TANG Z, LI Y B, et al. Food diversity and choice of white-headed langur in fragmented limestone hill habitat in Guangxi, China[J]. Acta Ecol Sin, 2013, 33(2):109-113. doi:10.1016/j.chnaes. 2013.01.007.
    [28] PETIT R J, AGUINAGALDE I, DE BEAULIEU J L, et al. Glacial refugia:Hotspots but not melting pots of genetic diversity[J]. Science, 2003, 300(5625):1563-1565. doi:10.1126/science.1083264.
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闫晶晶,王雪鑫,范春节,陈祖旭,李梅,黄世能.广东省猴耳环遗传多样性研究[J].热带亚热带植物学报,2021,29(5):539~546

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History
  • Received:November 16,2020
  • Revised:January 08,2021
  • Online: September 23,2021
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