Stem Culture in vitro of Passiflora foetida and Salt Tolerance of Seedlings

Abstract:

In order to establish a rapid regeneration system of Passiflora foetida, the effect of plant growth regulators on adventitious bud induction, rooting were studied by using stems as explants, as well as the salt tolerance of seedlings. The results showed that the induction and growth of adventitious buds were suitable on MS+6-BA 0.5 mg/L+NAA 0.05 mg/L medium; the callus induction was appropriate on MS+6-BA 3.0 mg/L+ NAA 0.3 mg/L medium; and the rooting of plantlets was available on 1/2MS+IBA 0.2 mg/L medium. The survival rate of plantlets could up to 92.6% after transplanted on matrix (peat:vermiculite:perlite=2:1:1). The growth of P. foetida seedlings was not affected under 0-200 mmol/L NaCl, while plants appeared dwarfing, leaf wilting, and yellow under more than 200 mmol/L NaCl stress. The SOD activity in leaves increased with increment of NaCl concentration, while POD, CAT and APX activities increased at first, and then decreased. These would provide technical support for the breeding of P. foetida and the restoration of seashore ecology.

Key words:Passiflora foetida;Stem;Cluster shoot;Callus;Salt stress