Home > Archive>Volume 29, Issue 3, 2021 >276-284. DOI:10.11926/jtsb.4281
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Effect of Nitrogen Forms on Photosynthetic Characteristics and Nitrogen Metabolism of Clematis
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the Provincial Public Welfare Research Program of Zhejiang province (Grant No.LNG18C 150006); Science and Technology Program of Zhejiang province(Grant No.2019C02036).

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    Abstract:

    In order to understand the photosynthetic characteristics and response mechanism of nitrogen metabolism in Clematis, the growth, light response curve, A-Ci curve and nitrogen metabolism related enzyme activities of 1-year-old Clematis crassifolia and C. paten ssp. tientaiensis were studied under different ratios of NH4+-N:NO3--N. The results showed that the ratio of NH4+-N:NO3--N significantly affects the biomass and chlorophyll content of clematis, and the biomass, Chl a, Chl b, and Car contents of C. crassifolia reach the maximum under the ratio at 1:1, while the Chl a and Chl b contents of C. paten ssp. tientaiensis reach the maximum under the ratio at 1:3. Under the ratio of 1:1, the light saturation point (LSP) of C. crassifolia and the light compensation point (LCP) of C. paten ssp. tientaiensis were the maximum, and the Vcmax and Jmax values of C. crassifolia leaves were significantly higher than those under other ratios. The glutamine synthetase (GS) activity of C. paten ssp. tientaiensis was the highest under the ratio of 3:1, while NR and nitrite reductase (NiR) activities under the ratio at 1:3 were significantly higher than those under other ratioes. Therefore, the mixed application of NH4+-N and NO3--N could effectively promote the growth and photosynthesis, and enhance the nitrogen utilization efficiency of two Clematis. C. crassifolia was suitable for 1:1 of NH4+-N to NO3--N, while C. paten ssp. tientaiensis was more suitable for 1:3.

    Reference
    [1] YU F H, JIANG J L, SUN L. Research progress on effects of different fertilizers on crop growth[J]. Mod Agric Sci Technol, 2018(4):9-10. doi:10.3969/j.issn.1007-5739.2018.04.006. 于富豪, 姜金玲, 孙璐. 不同肥料对作物生长的影响研究进展[J]. 现代农业科技, 2018(4):9-10. doi:10.3969/j.issn.1007-5739.2018.04.006.
    [2] Zou W T, Li H Z, Lin W J, et al. Effects of the content of available nitrogen in water on the growth and enzymatic activity of Brassica juncea[J]. J Anhui Agric Univ, 2011, 38(6):952-956. 邹文桐, 李海珍, 林文娇, 等. 氮素水平对宽杆芥菜幼苗生长及酶活性的影响[J]. 安徽农业大学学报, 2011, 38(6):952-956.
    [3] ROTHSTEIN D E, CREGG B M. Effects of nitrogen form on nutrient uptake and physiology of Fraser fir (Abies fraseri)[J]. For Ecol Manag, 2005, 219(1):69-80. doi:10.1016/j.foreco.2005.08.043.
    [4] XING Y, MA X H. Research progress on effect of nitrogen form on plant growth[J]. J Agric Sci Technol, 2015, 17(2):109-117. doi:10. 13304/j.nykjdb.2014.574. 邢瑶, 马兴华. 氮素形态对植物生长影响的研究进展[J]. 中国农业科技导报, 2015, 17(2):109-117. doi:10.13304/j.nykjdb.2014.574.
    [5] ABUZINADAH R A, READ D J. The role of proteins in the nitrogen nutrition of ectomycorrhizal plants:IV. The utilization of peptides by birch (Betula pendula L.) infected with different mycorrhizal fungi[J]. New Phytol, 1989, 112(1):55-60. doi:10.1111/j.1469-8137.1989.tb 00308.x.
    [6] WANG W C, LI L Q. A new system of classification of the genus Clematis (Ranunculaceae)[J]. Acta Phytotaxon Sin, 2005, 43(5):431-488. doi:10.1360/aps040130. 王文采, 李良千. 铁线莲属一新分类系统[J]. 植物分类学报, 2005, 43(5):431-488. doi:10.1360/aps040130.
    [7] GUAN K Y, LI Z J, LI J X, et al. A preliminary study on the introduction and cultivation of Clematis[J]. Acta Bot Yunnan, 2002, 24(3):392-397. doi:10.3969/j.issn.2095-0845.2002.03.014. 管开云, 李志坚, 李景秀, 等. 铁线莲属植物的引种栽培研究初报[J]. 云南植物学报, 2002, 24(3):392-397. doi:10.3969/j.issn.2095-0845.2002.03.014.
    [8] HUANG M, DUAN Y Q, LI Y J, et al. Effects of different nitrogen form ratios on the nitrogen accumulation and utilization of winter wheat[J]. J Soil Water Conserv, 2013, 27(6):290-293,304. doi:10. 3969/j.issn.1009-2242.2013.06.056. 黄明, 段有强, 李友军, 等. 不同氮素形态配比对冬小麦氮素积累和利用的影响[J]. 水土保持学报, 2013, 27(6):290-293, 304. doi:10.3969/j.issn.1009-2242.2013.06.056.
    [9] TAN J K, ZHANG Y L, SHEN Q R, et al. Effects of different NH4+-N/NO3--N ratios on water use efficiency and its biological characteristics of rice at early stage[J]. J Nanjing Agric Univ, 2002, 25(3):49-52. doi:10.3321/j.issn:1000-2030.2002.03.012. 谈建康, 张亚丽, 沈其荣, 等. 不同形态氮素比例对水稻苗期水分利用效率及其生物效应的影响[J]. 南京农业大学学报, 2002, 25(3):49-52. doi:10.3321/j.issn:1000-2030.2002.03.012.
    [10] LIU R, SHI F, LIU W C, et al. Effect of nitrogen forms on cell ultrastructure and photosynthesis of tomato under salinity[J]. Acta Hort Sin, 2015, 34(3):471-479. doi:10.16420/j.issn.0513-353x.2014-0821. 刘冉, 石峰, 刘伟成, 等. 不同形态氮素对盐胁迫下番茄细胞超微结构与光合作用的影响[J]. 园艺学报, 2015, 34(3):471-479. doi:10.16420/j.issn.0513-353x.2014-0821.
    [11] Liu Y H. Studies in mechanism of ammonium toxicity in tomato (Lycopersicon esculentum Mill.) under different light intensities[D]. Hangzhou:Zhejiang University, 2004:1-56. 刘永华. 不同光强下番茄铵毒害机理的研究[D]. 杭州:浙江大学, 2004:1-56.
    [12] XIONG Q E. Plant Physiology[M]. Chengdu:Sichuan Science and Technology Press, 2003:122-128. 熊庆娥. 植物生理学实验教程[M]. 成都:四川科学技术出版社, 2003:122-128.
    [13] LONG S P, BERNACCHI C J. Gas exchange measurements, what can they tell us about the underlying limitations to photosynthesis? Procedures and sources of error[J]. J Exp Bot, 2003, 54(392):2393-2401. doi:10.1093/jxb/erg262.
    [14] GAO J F. Experimental Guidance for Plant Physiology[M]. Beijing:Higher Education Press, 2006:74-77. 高俊凤. 植物生理学实验指导[M]. 北京:高等教育出版社, 2006:74-77.
    [15] QIAO F L. Determination Technique of Plant Physiology Experiment Analysis[M]. Beijing:China Agricultural Science and Technology Press, 2002:78-82. 乔富廉. 植物生理学实验分析测定技术[M]. 北京:中国农业科学技术出版社, 2002:78-82.
    [16] LOULAKAKIS K A, ROUBELAKIS-ANGELAKIS K A. Intracellular localization and properties of NADH-glutamate dehydrogenase from Vitis vinifera L.:Purification and characterization of the major leaf isoenzyme[J]. J Exp Bot, 1990, 41(10):1223-1230. doi:10.1093/jxb/41.10.1223.
    [17] MA Z Y, SHAO L H, LIU L F, et al. Corn leaves glutamate synthase (GOGAT) purification and characteristic research [J]. J Maize Sci, 2009, 17(5):82-84. 马振勇, 邵立红, 刘利锋, 等. 玉米叶片谷氨酸合酶(GOGAT)的纯化和特性研究[J]. 玉米科学, 2009, 17(5):82-84.
    [18] SUN S Q, YAN X F. Determination method of nitrate reductase activity in the leaves of Camptotheca acuminata[J]. J NE For Univ, 2004, 32(3):83-84. doi:10.3969/j.issn.1000-5382.2004.03.027. 孙世芹, 阎秀峰. 喜树叶片硝酸还原酶活性的测定方法[J]. 东北林业大学学报, 2004, 32(3):83-84. doi:10.3969/j.issn.1000-5382. 2004.03.027.
    [19] Shanghai Institute of Plant Physiology, Chinese Academy of Sciences. Guide to Modern Plant Physiology Experiments[M]. Beijing:Science Press, 1999:261-262. 中国科学院上海植物生理研究院, 上海市植物生理学会. 现代植物生理学实验指南[M]. 北京:科学出版社, 1999:261-262.
    [20] XU J L, BIE Z L, ZHANG S L. Effects of nitrogen form ratios on the lettuce growth and activity of protective enzymes[J]. J Huazhong Agric Univ, 2005, 24(3):290-294. doi:10.3321/j.issn:1000-2421.2005. 03.018. 徐加林, 别之龙, 张盛林. 不同氮素形态配比对生菜生长、品质和保护酶活性的影响[J]. 华中农业大学学报, 2005, 24(3):290-294. doi:10.3321/j.issn:1000-2421.2005.03.018.
    [21] GAO Z, XU Y C, Shen Q R, et al. Compound fertilizers with different nitrogen forms on the growth and nitrogen use efficiency of maize seedlings[J]. Acta Agric Boreali-Sin, 2005, 20(6):68-72. doi:10. 3321/j.issn:1000-7091.2005.06.018. 高志, 徐阳春, 沈其荣, 等. 不同氮素形态配比的复混肥对玉米苗期生长及氮肥利用效率的影响[J]. 华北农学报, 2005, 20(6):68-72. doi:10.3321/j.issn:1000-7091.2005.06.018.
    [22] WANG G Y. Effects of different nitrogen forms and combined with plant hormone on growth, nutrient uptake and their distribution in tobacco plants[D]. Baoding:Hebei Agricultural University, 2002:19-28. 王国英. 不同形态氮素及植物激素对烟草生长、养分吸收和分配的影响[D]. 保定:河北农业大学, 2002:19-28.
    [23] ZHANG J Z, LIN G Z, LIN Z F. et al. Response of photosynthesis to growth light intensity in some south subtropical woody plants[J]. J Trop Subtrop Bot, 2005, 13(5):413-418. doi:10.3969/j.issn.1005-3395. 2005.05.009. 张进忠, 林桂珠, 林植芳, 等. 几种南亚热带木本植物光合作用对生长光强的响应[J]. 热带亚热带植物学报, 2005, 13(5):413-418. doi:10.3969/j.issn.1005-3395.2005.05.009.
    [24] SHARP R E, MATTHEWS M A, BOYER J S. Kok effect and the quantum yield of photosynthesis:Light partially inhibits dark respiration[J]. Plant Physiol, 1984, 75(1):95-101. doi:10.1104/pp.75.1.95.
    [25] XING S Z, WANG J F, LI X L, et al. Different nitrogen fertilizers and ratios effect on growth, safety and quality of spinach[J]. J Plant Nutri Fertil, 2015, 21(2):527-534. doi:10.11674/zwyf.2015.0229. 邢素芝, 汪建飞, 李孝良, 等. 氮肥形态及配比对菠菜生长和安全品质的影响[J]. 植物营养与肥料学报, 2015, 21(2):527-534. doi:10.11674/zwyf.2015.0229.
    [26] ZHU X Q, JIA M, SHI P Y, et al. Fitting analysis of CO2 response curve of tobacco under different nitrogen fertilizer levels[J]. J S Agric, 2020, 51(3):537-544. doi:10.3969/j.issn.2095-1191.2020.03.007. 朱宣全, 贾孟, 史普酉, 等. 不同氮肥水平下烟草CO2响应曲线的拟合分析[J]. 南方农业学报, 2020, 51(3):537-544. doi:10.3969/j. issn.2095-1191.2020.03.007.
    [27] FARQUHAR G D, VON CAEMMERER S, BERRY J A. A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species[J]. Planta, 1980, 149(1):78-90. doi:10.1007/BF00386231.
    [28] WULLSCHLEGER S D. Biochemical limitations to carbon assimilation in C3 plants:A retrospective analysis of the A/Ci curves from 109 species[J]. J Exp Bot, 1993, 44(5):907-920. doi:10.1093/jxb/44.5.907.
    [29] LIU C N. Nutrition effects of different nitrogen on Larix gmelinii seedlings[D]. Harbin:Northeast Forestry University, 2010:43-50. 刘春娜. 落叶松幼苗对不同形态氮的营养响应[D]. 哈尔滨:东北林业大学, 2010:43-50.
    [30] LIN J F, LIN Y Z, QIU W C, et al. Comprehensive evaluation analysis on cultivation effects of lettuce under different nitrogen levels[J]. J Trop Subtrop Bot, 2013, 21(5):432-438. doi:10.3969/j.issn.1005-3395. 2013.05.009. 林俊芳, 林义章, 丘武昌, 等. 不同氮素水平对油麦菜栽培效果的综合评价分析[J]. 热带亚热带植物学报, 2013, 21(5):432-438. doi:10.3969/j.issn.1005-3395.2013.05.009.
    [31] YE L S, CHEN S L. Effects of nitrogen forms and ratios on photosynthetic characters and enzyme activities in nitrogen metabolism of Phyllostachys violascens[J]. Chin J Ecol, 2016, 35(9):2355-2360. doi:10.13292/j.1000-4890.201609.025. 叶莉莎, 陈双林. 氮素形态及配比对雷竹光合特性和氮代谢酶活性的影响[J]. 生态学杂志, 2016, 35(9):2355-2360. doi:10.13292/j. 1000-4890.201609.025.
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马晓华,胡青荻,章彦君,钱仁卷,郑坚,刘洪见.氮素形态对铁线莲光合特性及氮代谢的影响[J].热带亚热带植物学报,2021,29(3):276~284

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  • Received:July 02,2020
  • Revised:September 14,2020
  • Adopted:December 07,2020
  • Online: May 26,2021
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