Abstract:In order to understand the function of SCL3 (scarecrow-like 3) gene, a SCL3 gene was cloned from Brassica olreacea var. italic, named BoSCL3, which full length of cDNA sequence was 1355 bp and encoding 446 amino acids. The molecular weight of BoSCL3 was 49.96 KD, predicted to be hydrophobic protein. Phylogenetic analysis showed that the SCL3 in B. olreacea var. italica (BoSCL3) was closely related to those in B. rapa and B. napus, and SCL3 in the same family had high homology. The real-time fluorescence quantitative PCR analysis showed that the expression of BoSCL3 decreased at first and then increased with waterlogging stress time, indicating that this gene might be involved in response to waterlogging stress. Therefore, these would provide a theoretical basis for studying the molecular mechanism of BoSCL3 gene in response to waterlogging stress.
