Abstract:In oreder to understand the effective substances of water extract from Chrysanthemum morifolium flowers, its chemical constituents were identified by high-pressure liquid chromatography-high resolution mass spectrometry. The results showed that 376 chemical components were identified from flower water extract, and the relative content of 152 components were more than 0.1%. The components with high relative content included gypenoside XXVII, mimosine-O-β-glucoside, methyl-β-glucoside, d-1-O-methyl mucoinositol, l(+)-bornesitol, lucidol, methyl-β-galactoside, methyl-α-fructoside, ononitol, pinitol, (E)-2-hexenyl-α-arabinosyl-(1→2)-β-glucoside, miserotoxin, and so on. Therefore, the maim constituents in flower water extract of C. morifolium were flavonoids and alkaloids, which had 40 and 53 kinds with relative content of 22.68% and 26.57%, respectively, indicating that the main material basis for the performance of C. morifolium flowers.