Abstract:To provide the suggestion for pesticide application in snow fungus (Tremella fuciformis) cultivation, eight pesticides were used under spraying mode in different periods. The effects of pesticides on snow fungus strain Tr01 growth and health risk assessment of dietary exposure were studied. The results showed that all pesticides except prochloraz cream and pyridaben/acetamiprid microemulsion had not significant effects on growth of snow fungus. The level of pesticides residue was closely related to the type of pesticide and spraying mode. While the pesticide residue were below the corresponding MRL adopted from GB 2763-2019, the cHQ (chronic hazard quotient) of long-term dietary exposure for general population and young child ranged from 0.001 to 0.174 and 0.002 to 0.191, respectively. It indicated that the risk was acceptable. Based on the risk assessment, it was recommended for snow fungus cultivation that acephate, chlorpyrifos, and carbofuran should be banned, the use frequency of abamectin, prochloraz, and isoprocarb should be gradually reduced, and bifenthrin, acetamiprid, imidacloprid, and pyridaben could be used with the appropriate pre-harvest interval. These might be useful for designing good agricultural practices (GAP) and registering pesticide for snow fungus cultivation.