LIU Shan
Key Laboratory of Dry-hot Valley Characteristic Bio-Resources Development at University of Sichuan Province, Department of Biological and Chemical Engineering, Panzhihua University, Panzhihua 617000, Sichuan, ChinaSUN Rong
Key Laboratory of Dry-hot Valley Characteristic Bio-Resources Development at University of Sichuan Province, Department of Biological and Chemical Engineering, Panzhihua University, Panzhihua 617000, Sichuan, ChinaGAO Jing-lei
Key Laboratory of Dry-hot Valley Characteristic Bio-Resources Development at University of Sichuan Province, Department of Biological and Chemical Engineering, Panzhihua University, Panzhihua 617000, Sichuan, ChinaIn order to understand the effect of illumination on the expression of glucosyltransferase gene in Bougainvillea glabra, the cyclo-DOPA-5-glucosyltransferase gene (cDOPA 5GT) was cloned from bracts. The results showed that the full length cDNA of cDOPA 5GT was 1 446 bp, encoding 482 amino acids. The bioin-formatics analysis showed that isoelectric point of cDOPA 5GT was 5.77, showing an acidic and hydrophilic transmembrane protein without signal peptide. The cDOPA 5GT protein had characteristic motifs of glucosyl-transferase, and the secondary structure was dominant of α-helices and random coils, and the amino acid sequence was less conserved. The results of qRT-PCR and spectrophotometry showed that the expression of cDOPA 5GT gene and betacyanin accumulation in plants decreased significantly under shading by qRT-PCR and spectro-photometry. Therefore, it was suggested that the synthesis of betacyanin was regulated by glucosyltransferase gene, and positively correlated with light time.
刘姗,孙蓉,高静雷.三角梅cDOPA 5GT基因的克隆和光照对其表达的影响[J].热带亚热带植物学报,2021,29(1):75~81
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