Effects of Piriformospora indica on Seed Germination and Protocorm Growth of Dendrobium officinale
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    Abstract:

    In order to explore the effects of Piriformospora indica on seed germination and protocorm growth of Dendrobium officinale, the morphological and physiological characteristics were studied inoculated by P. indica on seeds and protocorm. The results showed that the initial germination time of D. officinale seeds inoculated with P. indica was earlier than the control, and the height, fresh weight, dry weight, number of leaves, leaf length, leaf width, root number and root length of protocorm of D. officinale inoculated with P. indica were higher than the control. The seed germination rate was 68.8% after 60 days of inoculation, which was higher than the control (28.6%). The chlorophyll a content, chlorophyll b content and total chlorophyll content in the protocorm of D. officinale inoculated for 80 days were significantly higher than the control, chlorophyll a/b was lower than the control, the net photosynthesis rate, stomatal conductance and transpirationrate were higher than the control, and the content of medicinal polysaccharide was significantly higher than the control. Therefore, the seed germination and protocorm growth of D. officinale were promoted by inoculation, and the quality and yield were improved.

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    [1] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China, Vol. 1[M]. Beijing:China Medical Science and Technology Press, 2015:282-283. 国家药典委员会. 中华人民共和国药典, 一部[M]. 北京:中国医药科技出版社, 2015:282-283.
    [2] SUN H, HU Q, JIN H, et al. Research advances in chemical consti-tuents and pharmacological activities of Dendrobii Officinalis Caulis[J]. Chin J Exp Trad Med Formul, 2017, 23(11):225-234. doi:10.13422/j.cnki.syfjx.2017110225. 孙恒, 胡强, 金航, 等. 铁皮石斛化学成分及药理活性研究进展[J]. 中国实验方剂学杂志, 2017, 23(11):225-234. doi:10.13422/j.cnki. syfjx.2017110225.
    [3] NG T B, LIU J Y, WONG J H, et al. Review of research on Dendro-bium, a prized folk medicine[J]. Appl Microbiol Biotechnol, 2012, 93(5):1795-1803. doi:10.1007/s00253-011-3829-7.
    [4] GUO Z B, ZHOU Y M, YANG J P, et al. Dendrobium candidum extract inhibits proliferation and induces apoptosis of liver cancer cells by inactivating Wnt/β-catenin signaling pathway[J]. Biomed Pharma-cother, 2019, 110:371-379. doi:10.1016/j.biopha.2018.11.149.
    [5] XIAO L, NG T B, FENG Y B, et al. Dendrobium candidum extract increases the expression of aquaporin-5 in labial glands from patients with Sjögren's syndrome[J]. Phytomedicine, 2011, 18(2-3):194-198. doi:10.1016/j.phymed.2010.05.002.
    [6] CHEN J, QI H, LI J B, et al. Experimental study on Dendrobium candidum polysaccharides on promotion of hair growth[J]. China J Chin Mat Med, 2014, 39(2):291-295. doi:10.4268/cjcmm20140225. 陈健, 戚辉, 李金标, 等. 铁皮石斛多糖促进毛发生长的实验研究[J]. 中国中药杂志, 2014, 39(2):291-295. doi:10.4268/cjcmm20140225.
    [7] VARMA A, VERMA S, SUDHA, et al. Piriformospora indica, a cultivable plant-growth-promoting root endophyte[J]. Appl Environ Microbiol, 1999, 65(6):2741-2744. doi:10.1128/AEM.65.6.2741-2744.1999.
    [8] VARMA A, BAKSHI M, LOU B G, et al. Piriformospora indica:A novel plant growth-promoting mycorrhizal fungus[J]. Agric Res, 2012, 1(2):117-131. doi:10.1007/s40003-012-0019-5.
    [9] SU Z Z, WANG T, SHRIVASTAVA N, et al. Piriformospora indica promotes growth, seed yield and quality of Brassica napus L.[J]. Microbiol Res, 2017, 199:29-39. doi:10.1016/j.micres.2017.02.006.
    [10] SHAH S, THAPA B B, CHAND K, et al. Piriformospora indica promotes the growth of the in-vitro-raised Cymbidium aloifolium plantlet and their acclimatization[J]. Plant Signal Behav, 2019, 14(6):1596716. doi:10.1080/15592324.2019.1596716.
    [11] PRASAD R, KAMAL S, SHARMA P K, et al. Root endophyte Piriformospora indica DSM 11827 alters plant morphology, enhances biomass and antioxidant activity of medicinal plant Bacopa monniera[J]. J Bas Microbiol, 2013, 53(12):1016-1024. doi:10.1002/jobm. 201200367.
    [12] CAO X X. Isolation and identification of pathogenic fungi causing diseases on Dendrobium officinale and the research of growth-promoting effect of Piriformospora indica[D]. Hangzhou:Zhejiang University, 2015. 曹星星. 铁皮石斛病原真菌分离与鉴定及印度梨形孢促生作用研究[D]. 杭州:浙江大学, 2015.
    [13] WU H F, SONG X Q, LIU H X. Ex-situ symbiotic seed germination of Dendrobium catenatum[J]. Acta Ecol Sin, 2012, 32(8):2491-2497. doi:10.5846/stxb201101280147. 吴慧凤, 宋希强, 刘红霞. 铁皮石斛种子的室内共生萌发[J]. 生态学报, 2012, 32(8):2491-2497. doi:10.5846/stxb201101280147.
    [14] STEWART S L, ZETTLER L W. Symbiotic germination of three semi-aquatic rein orchids (Habenaria repens, H. quinquiseta, H. macro-ceratitis) from Florida[J]. Aquat Bot, 2002, 72(1):25-35. doi:10.1016/S0304-3770(01)00214-5.
    [15] ZHOU Y, FU Q, WANG Y, et al. Effects of different hormones on seed sterile germination and cultivation of Bletilla striata[J]. Seed, 2019, 38(2):83-85,88. doi:10.16590/j.cnki.1001-4705.2018.02.083. 周雨, 付巧, 汪意, 等. 不同激素对白及种子无菌萌发生长的影响[J]. 种子, 2019, 38(2):83-85,88. doi:10.16590/j.cnki.1001-4705.2018.02.083.
    [16] LI H S. Principles and Techniques of Plant Physiological Biochemical Experiment[M]. Beijing:Higher Education Press, 2000:35-160. 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000:35-160.
    [17] JIN R, XIE W H, CHEN J B, et al. Effects of ultrasonic-assisted hot water extraction on the yield and structure of water soluble polysac-charides from Dendrobium officinale[J]. J Zhejiang Univ (Agric Life Sci), 2019, 45(2):196-204. doi:10.3785/j.issn.1008-9209.2018.04.161. 金蓉, 谢文华, 陈杰标, 等. 超声辅助热水提取对铁皮石斛水溶性多糖得率和结构的影响[J]. 浙江大学学报(农业与生命科学版), 2019, 45(2):196-204. doi:10.3785/j.issn.1008-9209.2018.04.161.
    [18] JIANG P, FAN L. The application of research methods in the symbiosis between orchids and fungi[J]. Mycosystema, 2009, 28(6):895-901. 姜鹏, 范黎. 兰科植物与真菌共生关系研究方法及其应用[J]. 菌物学报, 2009, 28(6):895-901.
    [19] GAO Y, CHEN Y H, XING X K. Symbiotic fungi inducing seed germination of medicinal Gymnadenia conopsea of Orchidaceae[J]. Mycosystema, 2019, 38(11):1948-1957. doi:10.13346/j.mycosystema. 190208. 高越, 陈艳红, 邢晓科. 兰科药用植物手参种子的真菌共生萌发[J]. 菌物学报, 2019, 38(11):1948-1957. doi:10.13346/j.mycosystema. 190208.
    [20] WANG W Y, ZOU H, DAI Y M, et al. Effect of Epulorhiza sp. on root morphology of Dendrobium officinale and their symbiotic relationship[J]. J Trop Subtrop Bot, 2020, 28(2):124-130. doi:10.11926/jtsb.4093. 王伟英, 邹晖, 戴艺民, 等. 瘤菌根菌对铁皮石斛根系形态的影响及其共生关系的研究[J]. 热带亚热带植物学报, 2020, 28(2):124-130. doi:10.11926/jtsb.4093.
    [21] KANG Z H, HAN S F, HAN Z M. Effects of Orchidaceous rhizoc-tonias on the growth of Dendrobium candidum[J]. J Nanjing For Univ (Nat Sci), 2007, 31(5):49-52. doi:10.3969/j.issn.1000-2006.2007.05.011. 亢志华, 韩素芬, 韩正敏. 兰科丝核菌类真菌对铁皮石斛生长的影响[J]. 南京林业大学学报(自然科学版), 2007, 31(5):49-52. doi:10.3969/j.issn.1000-2006.2007.05.011.
    [22] GAO W W, GUO S X. Effects of endophytic fungal hyphae and their metabolites on the growth of Dendrobium candidum and Anoectochilus roxburghii[J]. Acta Acad Med Sin, 2001, 23(6):556-559. 高微微, 郭顺星. 内生真菌菌丝及代谢物对铁皮石斛及金线莲生长的影响[J]. 中国医学科学院学报, 2001, 23(6):556-559.
    [23] YANG J W, CHEN X M, MENG Z X, et al. Effects of fungus S7(Tulasnella sp.) and extracts on the germination of Dendrobium can-didum seeds[J]. Sci Sin Vit, 2020, 50(5):559-570. 杨建文, 陈晓梅, 孟志霞, 等. 胶膜菌属真菌S7(Tulasnella sp.)及提取物对铁皮石斛种子萌发的影响[J]. 中国科学:生命科学, 2020, 50(5):559-570.
    [24] WANG Z C. Response mechanisms of Dendrobium officinale inoculated with Cladosporium halotolerans and key environmental factors for its seed germination[D]. Chongqing:Southwest University, 2019. 王兆春. 铁皮石斛接种枝孢霉菌的响应机制及其种子萌发关键环境因子研究[D]. 重庆:西南大学, 2019.
    [25] TEIXEIRA DA SILVA J A, TSAVKELOVA E A, ZENG S J, et al. Symbiotic in vitro seed propagation of Dendrobium:Fungal and bacterial partners and their influence on plant growth and development[J]. Planta, 2015, 242(1):1-22. doi:10.1007/s00425-015-2301-9.
    [26] WANG Y N, WANG L K, MIAO Z B, et al. Research advances in mycorrhizal fungi of Dendrobium (Orchidaceae)[J]. J Trop Subtrop Bot, 2013, 21(3):281-288. doi:10.3969/j.issn.1005-3395.2013.03.015. 王亚妮, 王丽琨, 苗宗保, 等. 兰科石斛属植物菌根真菌研究进展[J]. 热带亚热带植物学报, 2013, 21(3):281-288. doi:10.3969/j.issn. 1005-3395.2013.03.015.
    [27] SCHÄFER P, PFIFFI S, VOLL L M, et al. Manipulation of plant innate immunity and gibberellin as factor of compatibility in the mutualistic association of barley roots with Piriformospora indica[J]. Plant J, 2009, 59(3):461-474. doi:10.1111/j.1365-313X.2009.03887.x.
    [28] CHENG C Z, SUN X L, HAO X Y, et al. Effects of Piriformospora indica treatment on seed germination and seedling growth of longan plants[J]. Fujian J Agric Sci, 2018, 33(5):481-484. doi:10.19303/j. issn.1008-0384.2018.05.006. 程春振, 孙雪丽, 郝向阳, 等. 印度梨形孢对龙眼种子萌发和幼苗生长的影响[J]. 福建农业学报, 2018, 33(5):481-484. doi:10.19303/j.issn.1008-0384.2018.05.006.
    [29] JISHA S, SABU K K. Multifunctional aspects of Piriformospora indica in plant endosymbiosis[J]. Mycology, 2019, 10(3):182-190. doi:10.1080/21501203.2019.1600063.
    [30] ZHOU X Y, LIANG Y, DONG Z, et al. Effects of Piriformospora indica on growth and root morphology of Pinus thunbergii seedlings[J]. J Shandong Univ (Nat Sci), 2018, 53(7):7-14. doi:10.6040/j.issn. 1671-9352.0.2018.019. 周晓莹, 梁玉, 董智, 等. 印度梨形孢对黑松幼苗生长量及其根系形态的动态影响[J]. 山东大学学报(理学版), 2018, 53(7):7-14. doi:10.6040/j.issn.1671-9352.0.2018.019.
    [31] WU J D, CHEN Q, LIU X X, et al. Preliminary study on mechanisms of growth promotion in rice colonized by Piriformospora indica[J]. Chin J Rice Sci, 2015, 29(2):200-207. doi:10.3969/j.issn.1001-7216.2015.02.012. 吴金丹, 陈乾, 刘晓曦, 等. 印度梨形孢对水稻的促生作用及其机理的初探[J]. 中国水稻科学, 2015, 29(2):200-207. doi:10.3969/j. issn.1001-7216.2015.02.012.
    [32] WANG H L, ZHENG J R, ZHENG X D. Effect of Piriformospora indica on the growth and nutritional quality of Chinese celery (Apium graveolens L.)[J]. J Chin Inst Food Sci Technol, 2017, 17(6):124-130. doi:10.16429/j.1009-7848.2017.06.017. 王慧俐, 郑积荣, 郑晓冬. 印度梨形孢对芹菜的生长及营养品质的影响[J]. 中国食品学报, 2017, 17(6):124-130. doi:10.16429/j.1009-7848.2017.06.017.
    [33] LIN H F, XIONG J, ZHOU H M, et al. Growth promotion and disease resistance induced in Anthurium colonized by the beneficial root endo-phyte Piriformospora indica[J]. BMC Plant Biol, 2019, 19(1):40. doi:10.1186/s12870-019-1649-6.
    [34] LIU H C, LI M J, JIN L, et al. Effect of Piriformospora indica on tissue cultured seedling growth of Oncidium orchid[J]. Zhejiang Agric Sci, 2019, 60(4):642-645. doi:10.16178/j.issn.0528-9017.20190436. 刘慧春, 李明江, 金亮, 等. 印度梨形孢对文心兰组培苗生长的影响[J]. 浙江农业科学, 2019, 60(4):642-645. doi:10.16178/j.issn. 0528-9017.20190436.
    [35] YE W, SHEN C H, LIN Y L, et al. Growth promotion-related miRNAs in Oncidium orchid roots colonized by the endophytic fungus Pirifor-mospora indica[J]. PLoS One, 2014, 9(1):e84920. doi:10.1371/journal.pone.0084920.
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许凤来,朱志炎,何勇,田志宏.印度梨形孢对铁皮石斛种子萌发和原球茎生长的影响[J].热带亚热带植物学报,2021,29(1):59~66

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  • Received:April 03,2020
  • Revised:June 12,2020
  • Online: January 19,2021
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