Abstract:In order to understand the spore germination and protonema development of Bartramia pomiformis, the development process of haploid gametophyte cultured in laboratory was observed under microscope. The results showed that the spores began to germinate from the wall after 5 days, the protonema system was mainly composed of chloronema, and the axonema was differentiated from chloronema. The gametophyte was differentiated from the cells of axonema cultured after 22 days. According to Nishida's standard, the spore germination of B. pomiformis was Bryum-type. Therefore, these would provide the basic data of development for the artificial propagation of B. pomiformis.