Molecular Identification and Phenotype of Arabidopsis thaliana Mutant erd15

Abstract:

In order to explore the function of ERD15 gene by reverse genetics, a homozygous T-DNA insertion mutant of ERD15 gene of Arabidopsis thaliana was identified by PCR and semi-quantitative PCR. The phenotype of erd15 mutant was observed. The results showed that the number of rosette leaves in erd15 mutant increased significantly and flowering time was 3-4 days earlier, indicating that the erd15 mutant from vegetative to reproductive growth was earlier than the wild type. The main stem of wild-type A. thaliana was cylindrical with an average diameter of 1.29 mm, while that of erd15 mutant became flattened with an average diameter of 2.27 mm. Compared with the wild type, the carpel development of erd15 mutant fruit was affected, multiple rows of seed were arranged on the septum, and the length of silique was shortened by 37.67% with swelled apex, but the average seed number of silique increased in erd15 mutant. Therefore, it was suggested that ERD15 gene was involved in regulating the reproductive growth of A. thaliana.

Key words:Arabidopsis thaliana;ERD15;T-DNA insertion mutation;Reverse genetics