Isolation and Identification of Walnut Blight Pathogens and Resistance Evaluation of Walnut in Sichuan Basin Area
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  • YANG Han-bo

    YANG Han-bo

    Sichuan Provincial Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River & National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Rainy Area of West China Plantation Ecosystem Permanent Scientific Research Base, Institute of Ecology & Forestry, Sichuan Agricultural University, Chengdu 611130, China
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  • HAN Shan

    HAN Shan

    Sichuan Provincial Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River & National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Rainy Area of West China Plantation Ecosystem Permanent Scientific Research Base, Institute of Ecology & Forestry, Sichuan Agricultural University, Chengdu 611130, China
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  • HE Dan

    HE Dan

    Sichuan Provincial Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River & National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Rainy Area of West China Plantation Ecosystem Permanent Scientific Research Base, Institute of Ecology & Forestry, Sichuan Agricultural University, Chengdu 611130, China
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  • JIANG Shi-jiao

    JIANG Shi-jiao

    Sichuan Provincial Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River & National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Rainy Area of West China Plantation Ecosystem Permanent Scientific Research Base, Institute of Ecology & Forestry, Sichuan Agricultural University, Chengdu 611130, China
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  • CAO Guang-li

    CAO Guang-li

    Sichuan Provincial Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River & National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Rainy Area of West China Plantation Ecosystem Permanent Scientific Research Base, Institute of Ecology & Forestry, Sichuan Agricultural University, Chengdu 611130, China
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  • WAN Xue-qin

    WAN Xue-qin

    Sichuan Provincial Key Laboratory of Ecological Forestry Engineering on the Upper Reaches of the Yangtze River & National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River & Rainy Area of West China Plantation Ecosystem Permanent Scientific Research Base, Institute of Ecology & Forestry, Sichuan Agricultural University, Chengdu 611130, China
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    Abstract:

    In order to identify the pathogens of walnut blight in Sichuan Basin Area, the pathogens was isolated by using tissue isolation method, and the pathogenicity was tested using Koch's postulates. The pathogen was identified based on morphological characteristics and sequence analysis of 16S rDNA, and the resistance of 18 cultivars (clones) of Juglans regia was evaluated through field infection using the isolated strain. The results showed that the morphology of isolated pathogen were similar with Xanthomonas, which sequence of 16S rDNA showed 99% similarity with X. arboricola (GenBank No. KP340804.1). Therefore, X. arboricola was identified as the pathogen caused the walnut blight in Sichuan basin area. The incidence and disease index of 18 walnut cultivars (clones) were ranged from 35.07% to 78.57%, and 17.71% to 51.96%, with the coefficient of variation of 17.62% and 28.78%, respectively. Based on the resistance evaluation, five highly resistance cultivars (clones) were selected. Therefore, these would provide basis for pathogenesis mechanism of walnut blight and resistance breeding.

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杨汉波,韩珊,何丹,蒋时姣,曹广黎,万雪琴.四川盆地核桃黑斑病病原菌的分离、鉴定与核桃抗病性评价[J].热带亚热带植物学报,2020,28(5):487~494

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History
  • Received:December 31,2019
  • Revised:March 20,2020
  • Online: September 18,2020
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