Transcriptome Analysis of Chinese Ficus tikoua

Abstract:

Ficus tikoua is a woody plant used as traditional medicine. To investigate the genetic variation of F. tikoua cultivars, the gene expression profiles of Chinese F. tikoua were obtained by using the Illumina HiSeq 2500 platform. The results showed that a total of 197 362 transcripts were obtained from the transcriptome of F. tikoua, with a total and average length of 114 072 125 and 577 bp, respectively. There were 139 992 transcripts (70.93% of total transcripts) annotated by using BlastX and BlastN. There were 12 397, 12 340, 10 373, 94 431, 71 830, and 44 465 differentially expressed genes identified, and 126, 129, 125, 134, 138, and 137 metabolic pathways annotated from six pairs of tissue samples, blades and stems of three F. tikoua cultivars, respectively. So, these would help for understanding not only the genetic characteristics of F. tikoua, but also the metabolic pathway changes in different tissues.