Transcriptome Analysis of Chinese Ficus tikoua
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Project of China Scholarship Council (LJF[2017]50863), Program for National Innovation Training of Undergraduate (2018109760007, 2018109760008), Program for First-class Discipline Construction in Guizhou Province (QJKYF[2017]85), Project for Science and Technology of Guizhou Province (QKHJC[2017]1004), Project for Major Research by Innovation Group in Guizhou Education Department (QJHKY[2018]027), Project for Provincial Key Discipline of Ecology (ZDXK[2013]08), Project for Cooperative Base for Production, Education and Research of Organic Vegetable Ecological Cultivation and Breeding (KY[2013]125), Program for Innovative Training of Undergraduate in Guizhou (2018520777, 2018520778, 2018520831, 2018520861, 20195200341, 20195201373), Program for Educational Incubation of Guiyang University (GYU-KJT[2019]-06, GYU-KJT[2019]-07), and Discipline and Master's Site Construction Project of Guiyang University by Guiyang City Financial Support Guiyang University (SH-2020)

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    Abstract:

    Ficus tikoua is a woody plant used as traditional medicine. To investigate the genetic variation of F. tikoua cultivars, the gene expression profiles of Chinese F. tikoua were obtained by using the Illumina HiSeq 2500 platform. The results showed that a total of 197 362 transcripts were obtained from the transcriptome of F. tikoua, with a total and average length of 114 072 125 and 577 bp, respectively. There were 139 992 transcripts (70.93% of total transcripts) annotated by using BlastX and BlastN. There were 12 397, 12 340, 10 373, 94 431, 71 830, and 44 465 differentially expressed genes identified, and 126, 129, 125, 134, 138, and 137 metabolic pathways annotated from six pairs of tissue samples, blades and stems of three F. tikoua cultivars, respectively. So, these would help for understanding not only the genetic characteristics of F. tikoua, but also the metabolic pathway changes in different tissues.

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石登红,王继玥,白禹,张婷,刘燕,欧阳柏成.地果的转录组学分析[J].热带亚热带植物学报,2020,28(4):317~328

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History
  • Received:October 06,2019
  • Revised:December 04,2019
  • Online: July 23,2020
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