Identification and Fingerprint Construction of 19 New Hybrid Varieties (Lines) of Loquat by SSR

Abstract:

In order to understand the loquat (Eriobotrya japonica) hybrid authenticity and DNA fingerprint, 19 new loquat varieties or lines were analyzed by using SSR marker. Nineteen pairs SSR primers with clear and stable fragments were selected from eighty-nine previously published SSR. Totally, 83 amplified bands were detected from 24 materials of loquat, with an average of 4.37 for each primer pair. Polymorphism information content values ranged from 0.234 to 0.983, with an average of 0.764. The authenticity of all 19 new loquat varieties or lines were confirmed by 12 SSR markers with special bands of male parent, and the true hybrid rate was 100%. The genetic similarities among hybrids ranged from 0.728 to 0.969. The UPGMA dendrograms indicated that 19 hybrids could be get together into one big group (with the crossing parents ‘Xinbai No. 2’ and ‘Guifei’) and divided into 4 subgroups, and do not cluster by color of flesh. Furthermore, the DNA fingerprints for the 19 cultivars were established by 8 polymorphic SSR primers. So, these would provide references for cultivar identification, protection of new variety right and crossbreeding of loquat.

Key words:Loquat;Cross breeding;SSR marker;Hybrid identification;Fingerprint