Home > Archive>Volume 28, Issue 1, 2020 >70-77. DOI:10.11926/jtsb.4079
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Characteristics of Antioxidant Responses of Mangifera indica Leaves by Enhanced UV-B Radiation
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    Abstract:

    In order to understand the mechanism of antioxidant responses of mango leaves to enhanced UV-B radiation, the adult trees of Mangifera indica ‘Tainong No. 1’ were treated with enhanced UV-B radiation of 24 and 96 kJ/(m2·d), respectively, with the natural sunlight as control (CK), and the dynamic changes in physiological and biochemical indexes were studied. The results showed that MDA content, relative electrical conductivity, net photosynthetic rate, antioxidant enzyme activities, contents of polyphenols, Vc and mangiferin in leaves treated with 24 kJ/(m2·d) UV-B were not significantly different from CK, while flavonoids and reduced GSH contents were significantly higher than CK. MDA content, relative electrical conductivity, antioxidant enzyme activities, polyphenols, flavonoids, reduced GSH, mangiferin and other reduced protective components were significantly higher than CK treated with 96 kJ/(m2·d) UV-B, while net photosynthetic rate and Vc content were significantly lower than CK. Therefore, the adult mango trees has not been injured under 24 kJ/(m2·d) UV-B, probably through increasing the content of flavonoids and reduced GSH to scavenge reactive oxygen species. The leaves were damaged by reactive oxygen species treated with 96 kJ/(m2·d) UV-B, but it could still reduce the damage by two mechanisms, at first, enhancing the activities of antioxidant enzymes, such as SOD, POD, CAT, and increasing the contents of reducing components, such as polyphenols, flavonoids, reduced GSH, Vc and mangiferin, at the second, mangiferin, flavonoids and reduced GSH could absorb UV-B radiation.

    Reference
    [1] JANSEN M A K, GABA V, GREENBERG B M. Higher plants and UV-B radiation:Balancing damage, repair and acclimation[J]. Trends Plant Sci, 1998, 3(4):131-135. doi:10.1016/S1360-1385(98)01215-1.
    [2] ULM R, JENKINS G I. Q & A:How do plants sense and respond to UV-B radiation?[J]. BMC Biol, 2015, 3:45. doi:10.1186/s12915-015-0156-y.
    [3] SHINDELL D T, RIND D, LONERGAN P. Increased polar stratospheric ozone losses and delayed eventual recovery owing to increasing green-house-gas concentrations[J]. Nature, 1998, 392(6676):589-592. doi:10.1038/33385.
    [4] HAAPALA J K, MORSKY S K, SAARNIO S, et al. Carbon dioxide balance of a fen ecosystem in northern Finland under elevated UV-B radiation[J]. Glob Change Biol, 2009, 15(4):943-954. doi:10.1111/j. 1365-2486.2008.01785.x.
    [5] TERAMURA A H, SULLIVAN J H. Effects of UV-B radiation on photosynthesis and growth of terrestrial plants[J]. Photosynth Res, 1994, 39(3):463-473. doi:10.1007/bf00014599.
    [6] ROZEMA J, van de STAAIJ J, BJÖRN L O, et al. UV-B as an environmental factor in plant life:stress and regulation[J]. Trends Ecol Evol, 1997, 12(1):22-28. doi:10.1016/S0169-5347(96)10062-8.
    [7] STRID Å, CHOW W S, ANDERSON J M. UV-B damage and protect-tion at the molecular level in plants[J]. Photosynth Res, 1994, 39(3):475-489. doi:10.1007/BF00014600.
    [8] MORALES L O, TEGELBERG R, BROSCHÉ M, et al. Effects of solar UV-A and UV-B radiation on gene expression and phenolic accu-mulation in Betula pendula leaves[J]. Tree Physiol, 2010, 30(7):923-934. doi:10.1093/treephys/tpq051.
    [9] ZHOU K B, LI S J, YUAN M L. The influences of enhanced UV-B radiation on yield and fruits quality and photosynthesis of mango trees[J]. Chin J Trop Crops, 2018, 39(6):1102-1107. doi:10.3969/j.issn. 1000-2561.2018.06.010. 周开兵, 李世军, 袁孟玲. 增强UV-B辐射对芒果株产和果实品质及光合作用的影响[J]. 热带作物学报, 2018, 39(6):1102-1107. doi:10.3969/j.issn.1000-2561.2018.06.010.
    [10] YUAN M L, YUE K, WANG H, et al. Effects of enhanced UV-B radiation treatment on photosynthesis, yield and conventional quality of adult mango tree[J]. J S Agric, 2018, 49(5):930-937. doi:10.3969/j.issn.2095-1191.2018.05.15. 袁孟玲, 岳堃, 王红, 等. 增强UV-B辐射对芒果成年树光合作用及其产量与常规品质的影响[J]. 南方农业学报, 2018, 49(5):930-937. doi:10.3969/j.issn.2095-1191.2018.05.15.
    [11] LI H S. Principles and Techniques of Plant Physiological Biochemical Experiment[M]. Beijing:Higher Education Press, 2000:134-138. 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000:134-138.
    [12] CHEN A K, HAN R H, LI D Y, et al. A comparison of two methods for electrical conductivity about plant leaves[J]. J Guangdong Educ Inst, 2010, 30(5):88-91. doi:10.3969/j.issn.1007-8754.2010.05.016. 陈爱葵, 韩瑞宏, 李东洋, 等. 植物叶片相对电导率测定方法比较研究[J]. 广东教育学院学报, 2010, 30(5):88-91. doi:10.3969/j.issn. 1007-8754.2010.05.016.
    [13] ZHANG S Q. Experimental Techniques of Plant Physiology[M]. Beijing:Science Press, 2011:191-194. 张蜀秋. 植物生理学实验技术教程[M]. 北京:科学出版社, 2011:191-194.
    [14] CHEN J X, WANG X F. Experimental Guidance of Plant Physiology Experiment[M]. Guangzhou:South China University of Technology Press, 2006:76-77. 陈建勋, 王晓峰. 植物生理实验学实验指导[M]. 广州:华南理工大学出版社, 2006:76-77.
    [15] ZHOU K B, WANG H, MEI W L, et al. Antioxidant activity of papaya seed extracts[J]. Molecules, 2011, 16(6):6179-6192. doi:10.3390/molecules16086179.
    [16] FENG X, WANG S B, DENG J G, et al. Simultaneous determination of mangiferin and high mangiferin in mango leaves by high performance liquid chromatography[J]. Chin Tradit Pat Med, 2008, 30(10):1504-1506. doi:10.3969/j.issn.1001-1528.2008.10.030. 冯旭, 王胜波, 邓家刚, 等. 高效液相色谱法同时测定芒果叶中芒果苷与高芒果苷的含量[J]. 中成药, 2008, 30(10):1504-1506. doi:10.3969/j.issn.1001-1528.2008.10.030.
    [17] YANG L, ZHAO T H. The effect of enhanced UV-B radiation on soybean root's activate oxygen metabolism and antioxidative system[J]. N China Agric J, 2018, 33(5):174-180. doi:10.7668/hbnxb.2018.05.024. 杨璐, 赵天宏. UV-B辐射增强对大豆根系活性氧代谢及抗氧化系统的影响[J]. 华北农学报, 2018, 33(5):174-180. doi:10.7668/hbnxb.2018.05.024.
    [18] TEVINI M, BRAUN J, FIESER G. The protective function of the epidermal layer of rye seedlings against ultraviolet-B radiation[J]. Photochem Photobiol, 1991, 53(3):329-333. doi:10.1111/j.1751-1097.1991.tb03636.x.
    [19] YUAN M L, YUE K, WANG H, et al. Damages and responses on antioxidance of ‘Tainong No. 1’ Mango adult trees under treatment of enhanced UV-B radiation[J]. Chin J Trop Crops, 2018, 39(9):1724-1731. doi:10.3969/j.issn.1000-2561.2018.09.007. 袁孟玲, 岳堃, 王红, 等. ‘台农一号’芒果成年树在增强UV-B辐射处理下的损伤及其抗氧化响应[J]. 热带作物学报, 2018, 39(9):1724-1731. doi:10.3969/j.issn.1000-2561.2018.09.007.
    [20] ZHOU K B, LI S J, YUAN M L, et al. The injures and the responses on antioxidation of adult mango trees under the treatments of enhanced UV-B radiation[J]. Acta Hort Sin, 2019. doi:10.16420/j.issn.0513-353x.2018-0448. 周开兵, 李世军, 袁梦玲, 等. 杧果成年树在增强UV-B辐射处理下的损伤与抗氧化响应[J]. 园艺学报, 2019. doi:10.16420/j.issn.0513-353x.2018-0448.
    [21] LIU P, ZHOU K B, WANG S, et al. Damages and protection responses of Mangifera indica L. leaves with different age under enhanced UV-B radiation[J]. J Trop Org, 2010, 1(4):350-356. doi:10.3969/j.issn.1674-7054.2010.04.011. 刘鹏, 周开兵, 王爽, 等. 芒果不同叶龄叶片在增强UV-B辐射处理下的损伤和保护响应[J]. 热带生物学报, 2010, 1(4):350-356. doi:10.3969/j.issn.1674-7054.2010.04.011.
    [22] MURPHY T F. Effect of broad-band ultraviolet and visible radiation on hydrogen peroxide formation by cultured rose cells[J]. Physiol Plant, 1990, 80(1):63-68. doi:10.1111/j.1399-3054.1990.tb04375.x.
    [23] LIU J C, ZHAO Y J, SONG H Y, et al. Antagonism or synergism? combined effects of enhanced UV-B radiation and acid rain on photo-synthesis in seedlings of two C4 plants[J/OL]. Acta Ecol Sin, (2019-01-02). doi:10.1016/j.chnaes.2018.12.009.
    [24] DU Z K. The Physio-ecological effects and molecular mechanisms of enhanced UV-B radiation on peanut (Arachis hypogaea L.)[D]. Chong-qing:Southwest University, 2014:1-121. 杜照奎. UV-B辐射增强对花生的生理生态影响及其分子机制[D]. 重庆:西南大学, 2014:1-121.
    [25] ZHOU X M, ZHANG Z W, HUI Z M, et al. Effects of enhanced ultraviolet-B radiation on diurnal variations of photosynthesis in grape-vine[J]. Trans Chin Soc Agric Eng, 2009, 25(3):209-212. 周新明, 张振文, 惠竹梅, 等. UV-B辐射增强对葡萄光合作用日变化的影响[J]. 农业工程学报, 2009, 25(3):209-212.
    [26] HIDEG É, JANSEN M A K, STRID Å. UV-B exposure, ROS, and stress:Inseparable companions or loosely linked associates?[J]. Trends Plant Sci, 2013, 18(2):107-115. doi:10.1016/j.tplants.2012.09.003.
    [27] CZÉGÉNY G, LE MARTRET B, PÁVKOVICS D, et al. Elevated ROS-scavenging enzymes contribute to acclimation to UV-B exposure in transplastomic tobacco plants, reducing the role of plastid peroxi-dases[J]. J Plant Physiol, 2016, 201:95-100. doi:10.1016/j.jplph.2016.05.026.
    [28] KULKARNI V M, RATHOD V K. Mapping of an ultrasonic bath for ultrasound assisted extraction of mangiferin from Mangifera indica leaves[J]. Ultrason Sonochem, 2014, 21(2):606-611. doi:10.1016/j. ultsonch.2013.08.021.
    [29] DU S Y, LIU H R, LEI T T, et al. Mangiferin:An effective therapeutic agent against several disorders (Review)[J]. Mol Med Rep, 2018, 18(6):4775-4786. doi:10.3892/mmr.2018.9529.
    [30] TAN A X, ZHU Y B, LI X Y. Effect of mangiferin on antioxidant system in rats with cerebral ischemia reperfusion[J]. Lishizhen Med Mat Med Res, 2012, 23(1):161-162. doi:10.3969/j.issn.1008-0805.2012.01.071. 谭安雄, 朱耀斌, 李小云. 芒果苷对脑缺血再灌注大鼠抗氧化系统的影响[J]. 时珍国医国药, 2012, 23(1):161-162. doi:10.3969/j.issn. 1008-0805.2012.01.071.
    [31] LI L, JIANG X F, ZHANG Z B, et al. Study on antioxidant activity of crude extract of suede and mango skin[J]. J Univ Electr Sci Technol, 2003, 32(6):755-759. doi:10.3969/j.issn.1001-0548.2003.06.041. 李琳, 姜新发, 张志斌, 等. 枇杷皮和芒果皮粗提液抗氧化活性研究[J]. 电子科技大学学报, 2003, 32(6):755-759. doi:10.3969/j.issn. 1001-0548.2003.06.041.
    [32] BERARDINI N, FEZER R, CONRAD J, et al. Screening of mango (Mangifera indica L.) cultivars for their contents of flavonol O-and xanthone C-glycosides, anthocyanins, and pectin[J]. J Agric Food Chem, 2005, 53(5):1563-1570. doi:10.1021/jf0484069.
    [33] LIAO H L, SU C L, HU X L, et al. New advanced research on the pharmacology of mangiferin[J]. J Pharm Pract, 2008, 26(3):161-162. doi:10.3969/j.issn.1006-0111.2008.03.001. 廖洪利, 苏春丽, 胡小兰, 等. 芒果苷药理研究新进展[J]. 药学实践杂志, 2008, 26(3):161-162. doi:10.3969/j.issn.1006-0111.2008.03.001.
    [34] DORTA E, GONZÁLEZ M, LOBO M G, et al. Screening of phenolic compounds in by-product extracts from mangoes (Mangifera indica L.) by HPLC-ESI-QTOF-MS and multivariate analysis for use as a food ingredient[J]. Food Res Int, 2014, 57:51-60. doi:10.1016/j.foodres. 2014.01.012.
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王红,岳堃,郭钰柬,杨成坤,周开兵. UV-B辐射诱导芒果叶片抗氧化响应研究[J].热带亚热带植物学报,2020,28(1):70~77

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  • Received:April 13,2019
  • Revised:May 17,2019
  • Online: January 15,2020
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